Supporting Information
Heino J., Nokela T., Soininen J., Tolkkinen M., Virtanen L. & Virtanen R. (2015) Elements of
metacommunity structure and community-environment relationships in stream organisms. Freshwater
Biology.
Table S1. The concentrations used in PCR reactions and information about pipetting leading to final
concentrations.
Fig. S1. Maps showing the locations of the study sites.
Fig. S2. The proportions of different bacterial groups of the total amount of sequence.
Table S1. The concentrations used in PCR reactions and information about pipetting leading to final
concentrations. The latter follow the instructions given in Thermo Scientific (Thermo Fisher Scientific
Inc., UK) Phusion Hot start II High-Fidelity DNA Polymerase product information sheet.
Component
20 µL rxn
50 µL rxn
Final concentration
H₂O
add to 20 µL
add to 50 µL
5X Phusion GC Buffer
4 µL
10 µL
1X
10 mm dNTPs
0.4 µL
1 µL
200 µM each
Primer A
X µL
X µL
0.5 µM
Primer B
X µL
X µL
0.5 µM
Template DNA
X µL
X µL
Phusion Hot Start II DNA polymerase (2U/µL)
0.2 µl
0.5 µl
0.02 U/µL
Fig. S1. Locations of the study sites in the three drainage basins (from Schmera et al., 2013), including
the Tenojoki drainage basin (A), the Iijoki drainage basins (B) and the Koutajoki drainage basin (C). Note
that all streams in the Tenojoki drainage basin are tributary streams draining into the main stem of the
River Tenojoki, and no study site is located in the main stem river. Map credits: Mira Grönroos.
Fig. S2. The proportions of different bacterial groups of the total amount of sequence in our samples from
the streams of the Tenojoki drainage basin. Figure (a) shows the major bacterial taxa and figure (b) shows
the main groups within Proteobacteria.
a)
Proteobacteria
Bacteroidetes
13%
Cyanobacteria
59%
16%
Firmicutes
Thermi
Gemmatimonadetes
Acidobacteria
Actinobacteria
b)
19%
Gammaproteobacteria
57%
24%
Betaproteobacteria
Alphaproteobacteria