Table S1: List of cis-regulatory elements identified on the 1.5 Kb upstream regulatory sequence of OSIPP3 by using PLACE database
Name of the site
Sequence
Position from TSS
Function
References
ABRELATERD1
ACGTG
-1335
Dehydration stress and senescence
Simpson et al., 2003
ARR1AT
NGAAT
Transcriptional activator
Sakai et al., 2000
CAAT BOX
CAAT
-1249, -1074, -947,
-473
-82, -866, -841, -824,
-192, +88
Binding with RNA transcription
factor
Shirsat et al., 1989
CCAC PB CORE
CCAC
-467, -118, -78
Pollen specific expression
Bate and Twell, 1998
Enhancer element of LAT52
TGTGG
-1350
Park et al., 2006;
Enhancer element of LAT56
TGTGA
-966
Required for high level expression
in mature pollen
Required for high level expression
in mature pollen
Transcription activator
TAAATC
-732
a putative transcription binding site
for transcription activator to initiate
gene transcription in rice anther
Liu et al., 2013
DOFCOREZM
AAAG
Core site required for binding of
Dof proteins
Yanagisawa and Schmidt, 1999
GTGANTG10
GTGA
-1231, -1096, -707,
-637, -206, -187,
-178, -66
-965,- 1333
Late pollen development
Twell et al., 1991
MYCCONSESUSAT
CANNTG
-849, -796, -452
Abe et al., 2003
POLLENLELAT52
AGAAA
-64, -185, -295, -709
transcriptional activators in
abscisic acid signaling
Pollen specific expression
ROOTMOTIFTAPOX1
ATATT
-808, -1275, -1366
Root specific expression
Elmayan and Tepfer, 1995
TATA BOX
TATATAAA
-27
Accurate initiation of transcription
Grace et al., 2004
Swapna et al., 2011
Bate and Twell, 1998
Supplementary Figures:
Fig S1:
Fig 1: DNA blot hybridization for confirmation of transgenic plants developed with different promoter deletion constructs. A)
Transgenic genomic DNA digested with Xho I (released the bar gene from the cassette) and hybridized with bar gene as probe to
confirm the presence of transgene. B) Selected transgenic plants genomic DNA digested with BstX I (T-DNA insertion number) and
hybridized with GUS probe to analyze the left border junction fragment analysis. NT- non-transformed control plant; Kb- Kilo base
pair. C) PCR analysis of OSIPP3 T2 transgenic plants with promoter + GUS gene-specific sequence primer pairs. M: 1 kb ladder; B:
water as template; 1-14: transgenic plants; C: non transformed control plant; Mx: master mix; P: plasmid as positive control