HealthView Nucleic Acid Stain
Cat. No. :
GN-NAS-100 (1ml/vial)
Recommended
use: :
5μl HealthViewTM Nucleic Acid Stain for 100ml agarose gel solution
Content :
HealthViewTM Nucleic Acid Stain ( 1.0ml )
Description:
HealthViewTM Nucleic Acid Stain is a new and safe nucleic acid stain reagent. It can be used
to replace the ethidium bromide stain (EtBr) for detecting double-strand DNA, single-strand
DNA and RNA in agarose gels. When HealthViewTM Nucleic Acid Stain bound to dsDNA, it
emits green fluorescence or red fluorescence when bound to ssDNA or RNA. HealthViewTM
Nucleic Acid Stain is as sensitive as EtBr, and the staining protocol is also similar to EtBr.
HealthViewTM Nucleic Acid Stain is unlike the EtBr, know as a strong mutagen, it causes
fewer mutations in Ames test, and has negative results in mouse marrow chromophilous
erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test,
too. So HealthViewTM Nucleic Acid Stain is a better choice instead of EtBr for detecting
nucleic acid in agarose gels.
Storage:
Store at room temperature.
Protocol:
1. Prepare 100 ml of agarose gel solution (concentration from 0.8%-3%) in a 250 ml flask.
Place the flask in the microwave, heat it until the solution is completely clear and no any
floating particles are visible.
2. Add 5μl HealthViewTM Nucleic Acid Stain to the gel solution. Swirl the flask gently to
mix the solution and avoid forming bubbles.
3. While the agarose solution cool down, pour it into the tray and put the comb teeth into the
solution.
4. Let the agarose gel cool until solidified (about 20-30 min). Load samples on the gel and
perform electrophoresis.
5. Detect the bands under UV illumination.
Notes :
1. The thickness of gel should be less than 0.5cm since thick gels may decrease sensitivity.
2. Repeated melt of gels containing HealthViewTM Nucleic Acid Stain may cause low
sensitivity.
3. HealthViewTM Nucleic Acid Stain allows visualization of DNA (>50 ng) in agarose gels
under visible light. This eliminates the need for exposure to UV light, which can nick and
damage DNA. The intact DNA fragments purified from agarose gel can increase the efficiency
of subsequent molecular biology manipulations such as cloning, transformation and
transcription.