S+ Scanner
S+ Scanner
Contoents
1.
Scanner Components ...................................................................................................................................................... 3
2.
Inserting chips..................................................................................................................................................................... 4
3.
Attaching objective lens ................................................................................................................................................. 5
4.
Attaching the Mercury Burner ..................................................................................................................................... 6
5.
Turning on the Scanner .................................................................................................................................................. 7
6.
S+ Scanner program ........................................................................................................................................................ 8
6.1
Main window .......................................................................................................................................................... 8
6.2
Scan control ............................................................................................................................................................ 8
6.3
Axis control ............................................................................................................................................................. 9
6.4
Setting dialog...................................................................................................................................................... 10
6.5
Select chip ............................................................................................................................................................ 11
6.6
How to scan? ...................................................................................................................................................... 11
2
S+ Scanner
1. Scanner Components
Protective
enclosure
The protective enclosure protects the user from exposure to strong lamp
Light source
In S+ scanner, mercury lamp is used as light source. Much of the intensity of
radiation, high voltage, and moving parts.
the mercury burner is expended in the near ultraviolet, with peaks of
intensity at 313, 334, 365, 406, 435, 546, and 578 nanometers. At other
wavelengths of visible light, the intensity is steady but not nearly so bright,
but still usable for blue excitation.
Filters
In S+ scanner, the following three filter sets are installed:
1.
DA/FI/TR/Cy5-A-000
Excitation: 387/485/559/649 nm
Emission : 440/521/607/700 nm
2.
DAPI-5060C-000
Excitation: 377 nm
Emission : 447 nm
3.
TXRED-4040C-000
Excitation: 562 nm
Emission : 624 nm
Objective lens
Camera
RGB color, pixel size 4.4 µm, resolution 1624x1224 at 15 FPS
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S+ Scanner
2. Inserting chips
The chip number and position are indicated as the following picture.
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S+ Scanner
3. Attaching objective lens
1.
Connect the adaptor and objective lens.
2.
Connect the objective lens and lens tube.
5
S+ Scanner
4. Attaching the Mercury Burner
1. Loosen the socket clamping screw ① using the provided
Allen screwdriver.
2. Hold the upper section of lamp housing and pull it upward
to remove the socket section.
★To prevent malfunctions, do not hold the lamp housing by
the centering knobs ②.
3. Place the socket section upside down as shown in Fig.2
ⓞThe lamp housing is equipped with the holder for
transportation in the factory shipment condition or with an
old burner when the burner is replaced. Remove the holder
or old burner by loosening the two burner holding screws
③.
Fig.1
4. Attach the + (positive) pole of a specified mercury burner ④
to the fixed mount on the upper side, then the - (negative)
pole to the mount on the lower side.
★Be sure to use the USH102D, USH-103OL (mfd. by USHIO
Inc.) or HBO103W/2 (mfd. by OSRAM) burner.
Be careful and avoid leaving fingerprints or contaminants on
the mercury burner. Otherwise, there is a danger of explosion
due to distortion of glass caused by the stains. If the burner is
contaminated, clean it by wiping gently with gauze slightly
moistened with absolute alcohol.
5. Attach the socket section with burner to the original position
and tighten the clamping screw ①.
★Align the external edges of the lamp housing with those on
Fig.2
the socket section, and push the lamp housing straight
downward.
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S+ Scanner
5. Turning on the Scanner
1.
Make sure the slide door is closed.
2.
Plug the power cable and turn on the scanner power switch.
3.
Push the reset button.
4.
Turn on the control computer.
5.
Double click the S+ program icon.
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S+ Scanner
6. S+ Scanner program
6.1 Main window
The main window for the S+ Scanner program has all the features used to scan.
6.2 Scan control
These buttons are used to control camera play
and pause. If the camera is not connected, the
two buttons status will not be able.
This slide bar is used to control camera’s
exposure time. The unit is millisecond.
This button is used to start to scan.
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S+ Scanner
This button is used to show the region of
interest.
When
auto-focusing
function
is
started, the scanner will find focus on that
region.
This button is used to set the parameters for
auto-focusing function
This button is used to turn on/off the fan
This button is used to move the chip holder to
the door
6.3 Axis control
Push the move buttons(X+/X-, Y+/Y-, Z+/Z-,)
and the axis will move in the speed which is
indicated in the velocity text box.
This part is used to set the chip reference
points (left-top point, left-bottom point and
right-top point) and layout. “Insert” button is
used to insert the position. ‘Sing click’ will input
the position which is indicated in text box.
‘Double
click’
will
input
the
current
axis
position. The axis will be moved to the position
which is indicated in text boxes when ‘Move’
button is clicked. Set the layout(spot number)
using ‘X spots’ and ‘Y spots’ buttons.
This part is used to open/save chip information
files.
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S+ Scanner
“R+” and “R-“ buttons are used to rotate the
filters. “Insert” button is used to insert the filter
position. ‘Sing click’ will input the position
which is indicated in text box. ‘Double click’ will
input the current filter position.
Find the home position (original point) by
pushing the home button. Click the stop button
to stop the current motion
The map is used for indicating the current
position. The original point is located in lefttop.
6.4 Setting dialog
Click
and the following dialog will be shown.
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S+ Scanner
Set the excitation wavelength and objective NA.
Set how many image will be captured to find
focused image.
Set the pause time between two motions while
scanning.
Set the scan speed.
Once ‘Apply’ button is pushed, all the parameters in
‘Setting’ dialog will be applied.
6.5 Select chip
After clicking
, the following dialog will be shown. This dialog is used to select and name
the chips.
6.6 How to scan?
11