GUIDED NOTES-GEL ELECTROPHORESIS What does gel electrophoresis do? What moves a further distant through the gel? What is an agarose gel? What is agarose gel submerged in? What is agarose placed in salt solution? Why is a blue dye (loading dye) added to DNA samples? What is the charge on DNA? What makes DNA the charge above? What pole does DNA move toward in the electrophoresis chamber? How are DNA fragments separated by size in agarose gel? What is the purpose of ethidium bromide? What are markers (ladders)? What other molecule can be separated using the gel electrophoresis technique? Do you place your DNA at the positive or negative end of Gel? What happens to DNA strands of the same length? What are bands on a DNA gel? What is the first step in gel electrophoresis? What do you need to make a gel? What do you mix together? What does the buffer do? Where is the mixture placed? What is second step? After gel solidifies what do you do? What is the next step? What should be the level of the buffer? What is the next step? What is needed to load DNA into wells? What is the next step? The black end has what charge? The red end has what charge? Which charge goes closest to the well? What should you check for to ensure your gel is running? What do you actually see running through the gel? What is used to stain the gel?