Week 22: January 28-1, 2013
Miss Rurode
AP Bio
Student Objectives [PA Standards]
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•
•
•
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Use Gel electrophoresis to separate digested DNA strands
Describe the successive levels of DNA packing in the chromatin structure
Identify specific ways gene expression can be regulated during transcription
Explain how DNA cloning is used to produce marketable products
Define and explain the use of the following in DNA technology; nucleic acid probe
hybridization, PCR, RFLP, genomic libraries, gel electrophoresis, DNA sequencing
Day
Mon.
D
Tues.
E
Wed.
A
Thurs.
B-LAB
Fri.
C-LAB
Class Activities
Homework
Assessment
• discuss gene
regulation resulting
in multicellular
organisms 
developmental
differentiation
• Discuss lytic and
lysogenic cycle of
viruses and viral
mutaions
• Transformation
labs due
TOMORROW
• Review emailed
slides
• Prelab teaching
(restriction enzymes
with a paper
plasmid)
• prelab quiz
• practice loading
wells
• run gel
electrophoresis lab
• review chapters 18
and 19 exam on 1821 next week
• come prepared for
lab tomorrow
• review chapters 18
and 19 exam on 1821 next week
• come prepared for
lab tomorrow
•stain gels and
analyze the results of
electrophoresis
•create logarithmic
graphs
• review chapters 18 • quality of
and 19 exam on 18- constructed graph
21 next week
and lab report
• come prepared for
lab tomorrow
• gel
electrophoreiss lab
due MON 2/11
• read chapter 18
about viruses skip
as advised by
reading guide
• read gel
electrophoresis lab
• participation in
review of
eukaryotic gene
expression
•performance on
exam next week
•performance on
exam next week
• engagement
during paper
plasmid activity
• participation in
pipet and well
loading
• quality of lab
results
Student
Materials
BRING
• review questions
RECEIVE
•answers to BIG
questions…maybe
BRING
RECEIVE
• updated gel
electrophoresis
lab
• biotechnology
assignment due
Wednesday 2/6
BRING
RECEIVE
• plasmid papers
BRING
RECEIVE
BRING
RECEIVE
• logarithmic
graphing paper