1
2
TABLE 1
3
Shewanella spp. and Pseudomonas spp. assessment [log CFU g-1 muscle]* in chilled salmon that was previously treated under
4
different hydrostatic high pressure conditions**
5
Chilling Storage Time
(days)
0
6
10
15
20
C
a 1.96 y
(0.16)
b 3.15
(0.16)
c 3.74
(0.14)
d 5.31 x
(0.09)
e 5.90 x
(0.06)
Shewanella spp.
T-1
T-2
a 0.00 z
a 0.00 z
(0.00)
(0.00)
b 2.66
b 3.51
(0.39)
(0.69)
c 3.55
bc 4.06
(0.34)
(0.66)
d 4.69 y
c 4.40 z
(0.03)
(0.08)
e 4.83 y
c 4.57 z
(0.04)
(0.09)
T-3
a 0.00 z
(0.00)
b 3.22
(0.09)
c 4.00
(0.28)
cd 4.46 z
(0.18)
d 4.59 z
(0.07)
C
a 1.30 y
(0.13)
b 3.46 y
(0.06)
c 4.94 x
(0.24)
cd 5.24 y
(0.06)
d 5.41
(0.13)
Pseudomonas spp.
T-1
T-2
a 0.00 z
a 0.00 z
(0.00)
(0.00)
b 2.74 z
b 2.48 z
(0.43)
(0.06)
c 3.94 zy
c 3.99 z
(0.45)
(0.11)
d 5.19 zy
d 4.81 z
(0.22)
(0.16)
e 5.58
e 5.49
(0.07)
(0.29)
T-3
a 0.00 z
(0.00)
b 3.58 y
(0.29)
c 4.52 yx
(0.53)
cd 5.11 zy
(0.14)
d 5.63
(0.15)
6
7
8
9
* Mean values of three independent determinations (n = 3) are expressed. Standard deviations are indicated in brackets. For each
10
microbial quality index and for each chilling time, mean values followed by different letters (z, y, x) denote significant
11
(p<0.05) differences among treatments. For each microbial quality index and for each treatment, mean values preceded by
12
different letters (a-e) denote significant (p<0.05) differences as a result of chilling time. No letters are included in cases of no
13
significant (p>0.05) differences.
14
15
** Treatment abbreviations: Control (C), 135 MPa for 30 s (T-1), 170 MPa for 30 s (T-2), 200 MPa for 30 s (T-3).
1
2
3
TABLE 2
Total volatile base-nitrogen (TVB-N) and trimethylamine-nitrogen (TMA-N) content [mg kg-1 muscle]* in chilled salmon that was
4
previously treated under different hydrostatic high pressure conditions**
5
Chilling Storage Time
(days)
0
6
10
15
20
TVB-N
C
T-1
T-2
T-3
a 289.6
a 282.8
a 286.2
a 289.6
(13.5)
(29.2)
(5.8)
(8.9)
ab 307.5
ab 301.4
ab 307.5
a 295.2
(8.5)
(1.6)
(33.0)
(8.5)
ab 313.1 y a 289.6 z a 308.6 zy b 319.9 y
(10.1)
(12.1)
(11.8)
(14.7)
b 313.5 y ab 304.2 zy a 291.8 z ab 294.1 zy
(8.9)
(22.4)
(9.7)
(15.6)
c 352.4 y b 335.0 zy b 338.4 z b 333.2 z
(1.9)
(17.6)
(2.4)
(7.8)
C
a 1.3
(0.1)
b 20.6 x
(0.4)
c 24.0 y
(2.3)
d 31.1 y
(2.5)
d 34.0 y
(5.1)
TMA-N
T-1
T-2
a 1.8
a 1.3
(0.0)
(0.1)
b 18.1 y
b 13.0 z
(0.1)
(1.2)
bc 20.6 zy c 17.8 z
(3.9)
(1.9)
c 27.2 zy
d 22.7 z
(6.2)
(1.5)
c 28.8 zy e 29.9 zy
(3.0)
(4.7)
T-3
a 1.6
(0.2)
b 13.9 z
(0.3)
c 17.6 z
(2.8)
cd 21.6 z
(3.1)
d 24.9 z
(3.7)
6
7
8
9
10
* Mean values of three independent determinations (n = 3) are expressed. Standard deviations are indicated in brackets. For both
11
quality indices and for each chilling time, mean values followed by different letters (z, y, x) denote significant (p<0.05)
12
differences among treatments. For each quality index and for each treatment, mean values preceded by different letters (a-e)
13
denote significant (p<0.05) differences as a result of chilling time. No letters are included in cases of no significant (p>0.05)
14
differences.
15
** Treatment abbreviations as expressed in Table 1.
1
2
3
TABLE 3
Thiobarbituric acid index (TBA-i) and fluorescence ratio assessment* in chilled salmon that was previously treated under different
4
hydrostatic high pressure conditions**
5
Chilling Storage Time
(days)
0
6
10
15
20
TBA-i
[mg malondialdehyde kg-1 muscle]
C
T-1
T-2
T-3
a 0.35 z
a 0.40 zy a 0.47 zy
a 0.61 y
(0.09)
(0.07)
(0.17)
(0.15)
b 0.58 z
b 0.62 zy a 0.56 zy ab 0.83 y
(0.06)
(0.13)
(0.10)
(0.19)
b 0.50 z
b 0.77 y
a 0.62 zy ab 0.79 y
(0.02)
(0.10)
(0.09)
(0.16)
b 0.71 z
b 0.78 zy
a 0.63 z
b 0.95 y
(0.21)
(0.28)
(0.18)
(0.12)
c 1.63
c 1.61
b 1.58
c 1.62
(0.10)
(0.12)
(0.03)
(0.15)
Fluorescence Ratio
C
a 0.22
(0.01)
bc 0.39
(0.09)
c 0.51 z
(0.04)
bc 0.45 z
(0.03)
b 0.40 z
(0.03)
T-1
a 0.23
(0.06)
ab 0.33
(0.09)
b 0.41 z
(0.06)
b 0.41 z
(0.08)
b 0.42 z
(0.07)
T-2
a 0.19
(0.05)
a 0.27
(0.06)
b 0.64 y
(0.05)
b 0.67 y
(0.13)
b 0.67 y
(0.11)
T-3
a 0.22
(0.08)
a 0.34
(0.06)
b 0.60 y
(0.04)
b 0.68 y
(0.06)
b 0.64 y
(0.02)
6
7
8
9
* Mean values of three independent determinations (n = 3) are expressed. Standard deviations are indicated in brackets. For each
10
chemical quality index and for each chilling time, mean values followed by different letters (z, y) denote significant
11
(p<0.05) differences among treatments. For each quality index and for each treatment, mean values preceded by different
12
letters (a-c) denote significant (p<0.05) differences as a result of chilling time. No letters are included in cases of no
13
significant (p>0.05) differences.
14
** Treatment abbreviations as expressed in Table 1.
1
2
3
TABLE 4
Alpha- and gamma-tocopherol content [mg kg-1 muscle]* in chilled salmon that was previously treated under different hydrostatic
4
high pressure conditions**
5
Chilling Time (days)
0
6
10
15
20
C
a 5.57
(0.83)
b 7.65
(0.57)
a 3.94
(1.95)
a 5.24
(1.12)
a 5.14
(1.75)
Alpha-tocopherol
T-1
T-2
ab 6.15
ab 5.92
(1.20)
(1.05)
b 7.62
b 7.20
(0.92)
(0.77)
a 5.22
ab 4.99
(0.97)
(2.30)
ab 6.25
a 5.23
(1.75)
(1.12)
ab 6.22
a 5.13
(0.98)
(0.90)
T-3
7.05
(1.52)
6.58
(1.21)
5.62
(0.40)
4.80
(1.25)
4.76
(1.47)
C
a 2.57
(0.24)
b 3.72
(0.18)
a 2.71
(0.48)
a 3.02
(0.34)
ab 3.19
(0.66)
Gamma-tocopherol
T-1
T-2
a 2.58
a 2.70
(0.40)
(0.32)
b 3.59
b 4.03
(0.34)
(0.52)
ab 3.22
a 2.63
(0.97)
(0.34)
b 3.72
ab 3.55
(0.40)
(0.67)
ab 3.16
ab 3.32
(0.55)
(0.79)
T-3
2.83
(0.32)
3.30
(0.54)
2.58
(0.50)
3.24
(0.24)
2.89
(0.37)
6
7
8
9
10
* Mean values of three independent determinations (n = 3) are expressed. Standard deviations are indicated in brackets. For each
11
tocopherol isomer and for each treatment, mean values preceded by different letters (a-b) denote significant (p<0.05)
12
differences as a result of chilling time; no letters are included in cases of no significant (p>0.05) differences. No significant
13
differences (p>0.05) were obtained as a result of previous high pressure treatment.
14
15
** Treatment abbreviations as expressed in Table 1.