P2B Bioprocess v.1 Introduction Biochemize S.L. is a biotechnological company focused in the design and development of processes based on biocatalysis. Our technological platform lies in the use of enzymes or microorganisms to perform chemical reactions able to substitute manufacturing processes based on classical organic chemistry. P2B Bioprocess v.1 Location We are located in Terrassa, at 20 km from Barcelona. Our office and laboratories are part of the facilities of the Technology Centre of our associate partner Leitat. P2B Bioprocess v.1 Our Technology Biochemize S.L. develops bioprocesses using biocatalysts (enzymes or microorganisms) in single or multiple transformation steps. Our technological platform P2B (Platform to Biochemize) is capable to perform high throughput screening of biocatalysts, reaction conditions, suitable substrates and products obtained. The screening can be done in a short period of time and renders information about different possible bioprocesses and conditions that could be further developed at a larger scale. P2B Bioprocess v.1 P2B – Platform To Biochemize P2B is the key to determine quickly with low risk and at very small scale if a conceptual biotransformation is technologically viable. Once the succeeding biocatalysts or bioprocesses have been identified, Biochemize S.L. investigates at lab scale the experimental conditions which can lead to the highest yields. Two approaches can be followed: P2B Bioprocess Development of bioprocesses suitable to substitute established synthetic procedures up to an industrial scale already P2B Discovery Perform biotransformations that can lead to a (new) chemical entity with improved features compared to the starting molecule P2B Bioprocess v.1 P2B – Bioprocess P2B Bioprocess is an approach to develop biotechnology procedures having advantages over synthetic processes: Specificity: bioprocesses are highly selective Non desired products can be avoided. Some synthetic steps can be by-passed though a direct selective transformation. A B C D A Bioprocess can be highly selective. Sometimes allows reducing synthetic pathways (e.g. protection/deprotection steps) P2B Bioprocess v.1 P2B – Bioprocess More advantages: Productivity / Cost effectiveness: Enzymes are high efficient catalysts and can be recovered and used several times Cost of Energy: Mild or moderate reaction conditions can be applied Less hazardous: Reagents and reaction conditions are less dangerous than in synthetic procedures Environmental friendly: Residues generated are less toxic and the disposal is usually more easy to manage P2B Bioprocess v.1 P2B – Bioprocess The information obtained from P2B-Bioprocess allows estimations of productivity, costs and risks needed to confirm whether the process is feasible at industrial scale. P2B Screening of biocatalysts & conditions Lab Scale Catalyst selection & conditions optimization Feasibility Industrialization Estimation of Productivity, Costs & Risks The development of a Bioprocess ranges from theoretical design to industrial scale-up and implementation. This latter phase is carried out by the customer under the support and supervision of Biochemize S.L. P2B Bioprocess v.1 P2B – Bioprocess transformations Oxidations / Reductions Hydration / Dehydration Glycosilations Sulfidations / Sulfoxidations Aromatic Chlorination N-, O-, S-methylation Nitrilation Alkylation / Dealkylation Amide and Ester formation / hydrolysis Potentially, any biotransformation already described… P2B Bioprocess v.1 P2B – Bioprocess operations Biochemize S.L. is able to perform many biocatalyzed transformations. Our most recent experience is based on the following reactions: OH O R CH2 OH Y R H R O O R C N OH R R O-R' Ester Formation & hydrolysis Transamidation Transesterification O R Amide Formation, Hydrolysis, Hydration & dehydration P2B Bioprocess v.1 Aldol reaction X Oxidation & Reduction Nitrile Formation & hydrolysis O N R' R'' Operations Biochemize S.L. offers the most outstanding and up to date technology options, while maintaining an industrial scope. If necessary, many operations can be outsourced to other companies and research centres under our direction and supervision. We have a powerful network of contacts in biocatalysis and bioprocessing. Several partnership agreements have been established. Advisors Analytical Services Biocatalyst Suppliers Bioprocessing Services Biotransformation Experts Enzyme Screening Enzyme Immobilization Genetic Engineering Manufacturers Research & Development Scaling-Up Services P2B Bioprocess v.1 Austria Bulgaria China Denmark Ireland Italy Korea Netherlands Spain United Kingdom United States Applications of P2B – Bioprocess Limit the use of organic solvents Avoid catalysts and reagents of toxicological concern Conduct reactions at mild or moderate temperature Skip protection/deprotection steps Source of building blocks Increase the IP of the company More… P2B Bioprocess v.1 Project Management in P2B – Bioprocess P2B Bioprocess projects are managed through a development by phases: Theoretical Evaluation PHASE I Experimental Exploratory Research Technology Development PHASE II PHASE III Scale-Up PHASE IV Industrial Implement. PHASE V This approach is: Flexible: Phases can be omitted, merged, delimited or adapted to each project or customer requirements. Step-wise: Each phase is not planned nor quoted until the previous phase is completed and the results presented. Risk-Control: The customer decides the project continuation at each phase depending on results and conclusions obtained so far. Results oriented: The target is to achieve the industrial implementation and a quick time to market. P2B Bioprocess v.1 Why contracting Biochemize? We offer the most outstanding and up to date technology options. Industrial scope guaranteed: productivity, costs and risks are the key drivers of our evaluations. Our technology platform (P2B) is a powerful tool for fast, low risk and small scale proof of concept verifications. We increase the success possibilities through the P2B platform and our network of contacts. Project management based on a development by phases approach. The customer controls the risks and costs associated to the project development. P2B Bioprocess v.1 Contact Jaume Mir Founder CEO and Commercial Director E-mail: jmir@biochemize.com Telephone: ++34 686 441 120 Address: C/. de la Innovació, 2 (Leitat Centre Tecnològic) 88225 – Terrassa (Barcelona) SPAIN P2B Bioprocess v.1 P2B Bioprocess v.1