NATURAL FIBROUS MATERIALS AS A SOURCE
OF INSPIRATION FOR THE DESIGN OF NOVEL
NANOBIOMATERIALS
Anna Mitraki
Laboratory of Natural Biomaterials
Department of Materials Science and Technology, University of Crete,
and
Institute for Electronic Structure and Lasers, FORTH
mitraki@materials.uoc.gr
Our research focuses on:
Rational design of protein fibrous materials
from the nano- to the macroscopic scale
using building blocks inspired from
Natural fibrous folds and hard-won biochemical
knowledge
Crystal structure of a
natural fibrous protein
made from sequence
repeats (blue squares)
[van Raaij et al. Nature
1999, 401:935]
50 nm
Peptide sequences as a
self-assembly system
Identification of minimal peptides corresponding to
natural building blocks (blue squares)
that can self-assemble into
fibrous objects
[
]n
B
50 nm
100 nm
Papanikolopoulou et al., J. Biol. Chem. 2005, 280 : 2481-2490
Modification of fibrous building blocks towards
specific functions:
-targeting sequences for metallization, cell attachment etc,
or
-Conjugation to other organic moieties to form hybrid
materials
Example:
Metal nanowires fabricated on an octapeptide fibrillar scaffold
displaying cysteines
Kasotakis et al., 2009, Biopolymers-Peptide Science, 92:164-72
….and
Development of inter-disciplinary approaches
towards controlled positioning on surfaces
and integration in devices
Example: peptide
fibrils positioned on
a hybrid organicinorganic material
using femtosecond
Laser Technologies
V. Dinca et al., Nanoletters, (2008) 8 (2): 528-543
A. Mitraki and M. Farsari, in : "Nanomaterials for Life Sciences" series,
vol. 15, pp 105-119, Wiley- VCH
Relevant recent publications:
1)
Dinca V., et al., (2008) Directed three-dimensional patterning of self-assembled peptide fibrils.
Nanoletters, 8: 538-543
2)
Dinca V., et al., (2008) Fabrication of amyloid peptide micro-arrays using Laser-induced forward
transfer and avidin-biotin mediated assembly Physica Status Solidi 5: 3576-3579
3)
Kasotakis, E., et al., (2009) Design of metal-binding sites onto self-assembled peptide fibrils
Biopolymers –Peptide Science 92: 164-172
4)
Tamamis, P., et al., (2009) Amyloid-like self-assembly of peptide sequences from the adenovirus fiber
shaft: insights from molecular dynamics simulations. J. Phys. Chem. B. 113: 15639-15647
5)
Mitraki, A. (2010) Protein aggregation: from inclusion bodies to amyloid and biomaterials. Advances in
Protein Chemistry and Structural Biology, 79: 89-125
6)
Sedman VL., et al., (2011) Surface-Templated Fibril Growth of Peptide Fragments from the Shaft
Domain of the Adenovirus Fibre Protein. Protein Pept Lett., 18: 268-274
7)
Tiggelaar, SM., et al., (2011). Neutron fibre diffraction studies of amyloid using H(2)O/D(2)O isotopic
replacement. Acta Crystallogr Sect F Struct Biol Cryst Commun. 67:332-335.
8)
Hyttel-Clausen C., et al., (2011) Electrostatic force microscopy of self-assembled peptide structures.
Scanning, in press
9)
Viguier, B., et al., (2011) Development of an electrochemical metal-ion biosensor using self-assembled
peptide nanofibrils. ACS Appl. Mater. Interfaces, in press.
10) Charalambidis, G., et al., 2011) Self-assembly into spheres of a hybrid diphenylalanine-porphyrin:
increased fluorescence lifetime, conserved electronic properties. Chemistry Eur. J., in press
Acknowledgements
UoC:
Emmanouil Kasotakis
Erifyli Kaloudi
Dina Terzaki
Alexandra Siakouli (EM Facility)
Eva Papadogiorgaki (EM Facility)
Aleka Manousaki (EM Facility)
Financial support:
EU “BeNatural” project
“Bioengineered Nanomaterials
For Research and Applications”
NMP4-CT-2006-033256
Coordinated by UoC
Collaborations
- IESL, FORTH CRETE
Dr. Maria Farsari, Prof. Costas Fotakis,
- Technical University of Denmark (DTU)
Prof. Winnie Svendsen, Dr. Jaime Castillo
- Institut Laue Langevin in Grenoble, France
Prof. Trevor Forsyth, Estelle Mossou
- University of Nottingham, England
Prof. Saul Tendler, Prof. Clive Roberts,
Dr. Stephanie Allen, Dr. Sedman Victoria
- University of Santiago de Compostela,Spain
Prof. Mark J. van Raaij
- University of Tel-Aviv, Israel
Prof. Ehud Gazit, Lihi Adler-Abramovich
- University of Cyprus
Prof. G. Archontis, Dr. Ph. Tamamis
- CEA Saclay, France
Dr. P. Guenoun, Cr. C. Chevallard