pGLO Lab Intro PPT

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IS THIS COOL?
IS THIS COOL?
IS THIS COOL?
Well that’s what we are going to do
Transgenesis
Transgenesis
• Multiple goals:
Transgenesis
• Multiple goals:
Transgenesis
• Multiple goals:
Transgenesis
• Multiple goals:
How does this happen?
Could we make insulin?
GFP (green fluorescent protein)
• Isolated from a jellyfish with this trait.
Why modify organisms with GFP?
Quick review quiz
Quick review quiz
1. What does LB have in it?
Quick review quiz
1. What does LB have in it?
2. What does lysogenic mean?
Quick review quiz
1. What does LB stand for?
2. What does lysogenic mean?
3. What does lyophilized mean?
Quick review quiz
1.
2.
3.
4.
What does LB have in it?
What does lysogenic mean?
What does lyophilized mean?
What is ampicillin?
Quick review quiz
1.
2.
3.
4.
5.
What does LB have in it?
What does lysogenic mean?
What does lyophilized mean?
What is ampicillin?
What is arabinose?
Quick review quiz
1.
2.
3.
4.
5.
6.
What does LB have in it?
What does lysogenic mean?
What does lyophilized mean?
What is ampicillin?
What is arabinose?
What is the purpose of the LB/amp/ara plate?
Quick review quiz
1.
2.
3.
4.
5.
6.
7.
What does LB have in it?
What does lysogenic mean?
What does lyophilized mean?
What is ampicillin?
What is arabinose do?
What is the purpose of the LB/amp/ara plate?
What is the purpose of the LB/amp plate?
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