Figi(Image J)操作流程
Jason
1.Open File
2.ImageDuplicate
3.If it is too dim
AdjustBrightness/Contrast
4.AutoMaximum(Adjust to the
appropriate value)Apply
5.AdjustThreshold
6. Adjust maximum to the
appropriate valueApply
7.BinaryWatershed
8. Analyze  Analyze Particles
9.Check this 4 squareOK
10.Save Summary, Results and
duplicated picture