Procedure to be handed out to students after completing the design.
Answer the first two questions on your worksheet before you proceed.
Materials
Item Amount 1. Chloroform and Ethanol (15%, 30%, 50%) each 1 tube; 2. Beetroot 1 pc; 3. Razor
blade 1 pc 4. Plastic chopping board 1 pc; 5. Test-tube 5 pcs; 6. Test-tube racks 1 pc; 7. Measuring
cylinder (10 ml) 1 pc; 8. Labels 4 pcs; 9. Forceps 1 pc; 10. Cork borer 1 pc; 11. Beaker (250 ml) 1 pc;
12. White tile 1 pc (for the whole group); 13. Distilled water (in wash bottle)
Procedure
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Transfer 10 ml of distilled water, Chloroform, 15%, 30%, and 50% of ethanol into
each of the 5 test tubes respectively. Label the tubes.
Using the same beetroot, prepare 4 cylinders of beetroot, each 2 cm long, using cork borer
and razor. Cut the cylinder of beetroot into 10 discs of the same thickness.
Rinse the cylinders of beetroot in running tap water until no pigment comes out from the
damaged cells.
Randomly put 2 discs of beetroot into each of the test tubes prepared in step 1.
Leave the tubes for 20 minutes. For every 5 minutes, shake the tube gently for a few seconds.
Take the cylinders of beetroot out from the test tubes using a pair of forceps.
Place the test tubes in front of a white sheet of paper. Record and compare the intensity of the
red colour of the solutions in the tubes with the naked eye.
Extension: One member from each group will use the test tube to measure the intensity of the
colour using a calorimeter. The data is shared by all the members within the group.
Construct a bar graph using the data.
Answer the questions in the worksheet.
Procedure to be handed out to students after completing the design.
Answer the first two questions on your worksheet before you proceed.
Materials
Item Amount 1. Chloroform and Ethanol (15%, 30%, 50%) each 1 tube; 2. Beetroot 1 pc; 3. Razor
blade 1 pc 4. Plastic chopping board 1 pc; 5. Test-tube 5 pcs; 6. Test-tube racks 1 pc; 7. Measuring
cylinder (10 ml) 1 pc; 8. Labels 4 pcs; 9. Forceps 1 pc; 10. Cork borer 1 pc; 11. Beaker (250 ml) 1 pc;
12. White tile 1 pc (for the whole group); 13. Distilled water (in wash bottle)
Procedure
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Transfer 10 ml of distilled water, Chloroform, 15%, 30%, and 50% of ethanol into
each of the 5 test tubes respectively. Label the tubes.
Using the same beetroot, prepare 4 cylinders of beetroot, each 2 cm long, using cork borer
and razor. Cut the cylinder of beetroot into 10 discs of the same thickness.
Rinse the cylinders of beetroot in running tap water until no pigment comes out from the
damaged cells.
Randomly put 2 discs of beetroot into each of the test tubes prepared in step 1.
Leave the tubes for 20 minutes. For every 5 minutes, shake the tube gently for a few seconds.
Take the cylinders of beetroot out from the test tubes using a pair of forceps.
Place the test tubes in front of a white sheet of paper. Record and compare the intensity of the
red colour of the solutions in the tubes with the naked eye.
Extension: One member from each group will use the test tube to measure the intensity of the
colour using a calorimeter. The data is shared by all the members within the group.
Construct a bar graph using the data.
Answer the questions in the worksheet.