Supplementary materials
Genome-wide transcriptional analysis of Saccharomyces cerevisiae during industrial bioethanol
fermentation
Fig. S1. Real-time RT-PCR validation. This microarray platform was validated by comparing the
microarray data with real time PCR results under four conditions, C0 (control), C1, C2, C3. (a)
Microarray results of selected genes. (b) Real-time RT-PCR results of selected genes. All data showed
here are fold changes for each gene under different conditions normalized by the expression under
control condition.
Fig. S2. Transcriptional responses of all genes at the late main fermentation stage in continuous and
fed-batch fermentation. Each point represents the log2 change of the mRNA level for a single gene at
36 h relative to 5 h under fed-batch fermentation on the vertical axis and the log2 change of the
mRNA level for the gene in the fifth fermentor relative to the first fermentor under continuous
fermentation on the horizontal axis.
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Table S1. Transcriptional responses of glucose repressible genes in continuous and fed-batch
fermentation.
Genes
GDH3
ACH1
GLK1
CIT2
HXT7,HXT6
GLC3
HXT13
HXT10
PFK26
GPX1
HAP4
GSY2
NDI1
PGM2
HXT14
HXT17
a
Continuous a
3
5th
2.2
1.8
1.2
1.2
0.1
1.4
0.2
1.5
4.4
6.0
1.0
2.8
2.2
3.5
2.8
3.7
2.8
4.0
3.2
5.8
1.5
4.9
2.2
3.6
1.2
0.8
1.5
3.8
2.3
3.9
1.6
2.3
rd
Fed-batch (h)b
24
36
2.5
2.6
1.3
1.6
0.9
1.3
1.8
2.7
1.2
1.9
1.0
1.5
1.1
2.4
1.6
1.7
1.9
2.5
2.8
2.7
2.6
3.9
1.7
1.9
0.3
0.9
2.1
2.8
1.2
0.8
1.2
2.2
data represent the log2(change fold) in samples from the 3rd and 5th fermentor based samples from
the 1st fermentor.
b
data represent the log2(change fold) in samples from 24 h, and 36 h based samples from 5 h.
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Fig. S1. (Li and Yuan, Genome-wide transcriptional analysis…)
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Fig. S2. (Li and Yuan, Genome-wide transcriptional analysis…)
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