OLLSCOIL NA hEIREANN, GAILLIMH
NATIONAL UNIVERSITY OF IRELAND, GALWAY
SEMESTER ONE EXAMINATION
AUTUMN 2000
SECOND SCIENCE
MICROBIOLOGY
EXTERN EXAMINER Professor C M Brown
INTERN EXAMINER Internal Examiners
TIME ALLOWED: 3 hours
ANSWER FIVE QUESTIONS
(INDICATE CLEARLY THE QUESTIONS ANSWERED ON THE FIRST
PAGE OF YOUR ANSWER BOOK)
1.
Write an essay on bacterial gene transfer mediated by phage.
2.
Write an essay entitled "RNA transcription in the bacterial cell."
3.
Describe the structure and function of the Gram-negative cell
envelope.
4.
Write an essay entitled "Bacterial endospores."
5.
Describe the quantitative measurement of microbial growth using
methods for determining cell numbers.
6.
Discuss the influences of osmotic concentration, water activity and salt
concentration on microbial growth.
7.
Write notes on two of the following:
(i) Substrate level phosphorylation.
(ii) Enzyme/substrate interactions and enzyme specificity.
(iii) Redox potential and biological redox reactions.
8.
Compare and contrast the microbial catabolism of glucose during
aerobic and anaerobic growth.