Product datasheet
Recombinant human PAK5 protein ab95311
2 Images
Overview
Product name
Recombinant human PAK5 protein
Protein length
Protein fragment
Description
Nature
Recombinant
Source
E. coli
Amino Acid Sequence
Accession
Q9P286
Species
Human
Molecular weight
34 kDa
Amino acids
425 to 719
Specifications
Our Abpromise guarantee covers the use of ab95311 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activity
Specific Activity: 4.2 nmoles/min/µg. Analysis of enzymatic activity: 1. Different concentrations of
PAK5 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM
EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20) at room temperature for
1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of
incubation at RT. 3. Fluorescence was detected using ?exc=460±40 nm and ?em=528±20 nm
filters.
Applications
Functional Studies
Purity
> 90 % by SDS-PAGE.
Form
Liquid
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: None
Constituents: 10% Glycerol, 5mM DTT, 25mM Tris HCl, 150mM Sodium chloride, pH 8.0
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This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
Function
The activated kinase acts on a variety of targets.
Tissue specificity
Predominantly expressed in brain.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated when activated by CDC42/p21.
Recombinant human PAK5 protein images
Image showing activity analysis of PAK5
(ab95311).
Functional Studies - PAK5 protein (Active)
(ab95311)
Image showing SDS-PAGE analysis of
ab95311.
1, 2, 3 : ab95311 at 6, 60 and 120 ug/ml.
SDS-PAGE - PAK5 protein (Active) (ab95311)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
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Valid for 12 months from date of delivery
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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