Product datasheet
Recombinant Human CrkRS protein ab204102
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Overview
Product name
Recombinant Human CrkRS protein
Protein length
Protein fragment
Description
Nature
Recombinant
Source
Baculovirus infected sf9 cells
Amino Acid Sequence
Accession
Q9NYV4
Species
Human
Sequence
KEQRTRHLLTDLPLPPELPGGDLSPPDSPEPKAITPPQQPYKKRPKICCP
RYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKK
VRLDNEKEGFPITAIREIKILRQLIHRSVVNMKEIVTDKQDALDFKKDKG
AFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLMEGLEYCHKKNFLH
RDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELL
LGEERYTPAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCP
AVWPDVIKLPYFNTMKPKKQYRRRLREEFSFIPSAALDLLDHMLTLDPSK
RCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSGVVV
EEPPPSKTSRKETTSGTSTEPVKNSSPAPPQPAPGKVESGAGDAIGLADI
TQQLNQSELAVLLNLLQSQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSI
SALTEATSQQQDSETMAPEESLKEAPSAPVILPSAEQTTLEASSTPADMQ
NILAVLLSQLMKTQEPAGSLEENNSDKNSGPQGPRRTPTMPQEEAAACPP
HILPPEKRPPEPPGPPPPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQ
PEAEPPGHLPHEHQALRPMEYSTRPRPNRTYGNTDGPETGFSAIDTDERN
SGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFAR
VPLALHPVVGQPFLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGL
HWGGPTQSSAYGKLYRGPTRVPPRGGRGRGVPY
Molecular weight
135 kDa including tags
Amino acids
658 to 1490
Tags
GST tag N-Terminus
Additional sequence information
NM_016507.
Specifications
Our Abpromise guarantee covers the use of ab204102 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Applications
SDS-PAGE
Western blot
Purity
> 70 % Densitometry.
Form
Liquid
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.5
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA,
0.004% DTT, 0.002% PMSF, 25% Glycerol
General Info
Function
Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit
of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation.
Regulates the expression of genes involved in DNA repair and is required for the maintenance
of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already
phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing,
possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly
leading to affect the response to estrogen inhibitors.
Tissue specificity
Ubiquitously expressed.
Involvement in disease
Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within
17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion
leading to trunctated CDK12 protein not in-frame with ERBB2.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX
subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylation at Thr-893 increases kinase activity.
Cellular localization
Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase.
Recombinant Human CrkRS protein images
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SDS-PAGE analysis of ab204102.
SDS-PAGE - Human CrkRS protein fragment
(ab204102)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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