Biotech Lab Safety
Material Safety Data Sheets
Hazards in the Lab
Chemical Classes
Bell Work
 Answer the following question in your lab
notebook:
Why do you think safety and
safety training is important for
laboratory work?
Objectives
 Identify specific biological/biohazardous/
chemical materials. (1.7)
 Students will locate the material safety data
sheets for the facility, and identify appropriate
internet MSDS resources.
 Students will apply knowledge of material
safety data sheets (MSDS) (1.3)
Key Terminology
 Material Safety Data Sheet (MSDS)
 Biohazard
 Safety Equipment
Safety Equipment
With your table group:
 Define Safety Equipment on your whiteboard.
 Be prepared to share your table’s definition.
Based on our class definition:
 Sort the Deck into “Safety” and “Non-Safety”
groups.
Introduction
 Science may be _______________, but can be
done safely when hazards are
________________.
 Everyone is ____________________: students,
teachers, supervisors, and higher administrators.
 All safety information brought to you today
applies to all science disciplines.
 Remember - each discipline has _____________
& ___________information and safety protocols.
Hazards in the Lab:
Biological Controls
 You will encounter biological materials throughout
this course.
 Wear proper __________ ___________ __________
 Universal Precautions: Treat everything like it is
infectious.
 Disinfect your lab bench each day:
 School provided disinfectant spray
 Freshly prepared solution of 1 part household bleach
and 10 parts water
 70% _____________________ (____________)
Hazards in the Lab:
Biological Hazards
 What could be encountered?
________________, fungi/mold
Injured student/ faculty
Human ___________sampling,
____________
 (not common in high school labs)
Hazards in the Lab?
 Where do you start?
 Gather Standard Operating Procedures and
other information for your facility
 M______________ S______________
D______________ S______________
Material Safety Data Sheet
 Here is the MSDS for Sodium chloride
(NaCl)
 What sections are found on an MSDS?
 1. Paste the MSDS into your Lab Notebook
 2. Highlight the sections listed on an MSDS
 Section 1 = ?
 Section 2 = ?
 Etc.
Material Safety Data Sheets
(MSDS)
 Contain all safety and use information about a
chemical.
 _________________are required to provide them
when chemicals are purchased.
 MSDS are an important part of _________Hazard
Communication Standard (1910.1200)
 MSDS must be readily available to ______________.
 Be sure to read all sections before using a chemical.
MSDS Activity Sheet
 Using the MSDS Activity Sheet:
 Think about the answers on the worksheet
 Pair up, and SHARE with your neighbor what
the answers would be….don’t write anything
yet!
Where’s the MSDS?
 Material Safety Data Sheets are required to be
present in ANY facility that uses a chemical.
 Where are ours?
 Record the location in your lab notebook
 You can even take a picture with your digital camera or phone
if the teacher says yes, and paste that in your lab notebook
Find One!
 Using the internet, or one of the MSDS
Binders, locate an MSDS for a chemical used
in our facility.
 Some chemicals used in our facility are:
Copper Sulfate Pentahydrate
LB Agar
LB Broth
Ethidium Bromide
Ethanol
http://www.flinnsci.com/search_MSDS.asp
http://www.fishersci.com/ Click MSDS Search
Sodium Hydroxide
Acetic Acid
TAE Buffer
Crystal Violet
Glucose
Thumbs Up-Thumbs Down
 Was the MSDS binder in an easily accessible
location?
Hazards in the Lab:
Chemical Classes
 Chemicals are segregated according to class:
 Acids
 Pyrophoric
 Bases
 Peroxide Forming
 Solvents
 Toxic
Flammables
 Oxidizers
Halogenated
 Information about Toxicity, oxidation, etc are
identified on the MSDS
Chemical Classes:
_____________
 Def: The pH range of acids is from 0.1- 6.9.
 Examples: hydrochloric acid (HCl), acetic
acid, nitric acid, phenol, sulfuric acid
 Storage Precautions:
 Store in large bottles of acids on low shelf
or in acid cabinets.
 Segregate acids from bases.
 Have ____________ _______ with
neutralizers and absorbent.
Proper Storage for Acids:
Inside Look at Acid Storage:
Improper Acid Storage
Rust
Chemical Classes:
_____________
 Def: The pH range of basic solutions is from
_________ -- _________
 Examples: sodium hydroxide (NaOH),
ammonium hydroxide
 Storage Precautions:
 Segregate bases from acids
 Have a spill kit with neutralizers and
absorbent.
Chemical Classes:
Flammables
 Def: Has a flash point of 60.5°C (141°F) or lower
 Examples: acetone, ethanol, methanol, isopropyl
alcohol
 Storage Precautions:
 Store in approved safety _______or ____________
 Segregate from oxidizing acids and oxidizers
 Keep away from any source of _____________:
flames, localized heat, or sparks.
 Store highly volatile flammable liquids in a
specially equipped refrigerator.
Example of Proper Storage:
Flammables
Chemical Classes:
Pyrophoric
 Def: A material which ignites spontaneously
upon exposure to air (or oxygen).
 Examples: Powders: calcium, zinc, lead,
iron, nickel
 Storage Precautions: Store ___________in a
cool, dry place
Chemical Classes:
Peroxide-Forming Chemicals
 Def: Organic compounds that spontaneously form
peroxides by a free-radical reaction with molecular
oxygen in a process of auto-oxidation.
 Examples: ethyl ether, isopropyl ether, acetaldehyde
 Storage Precautions:
 Store in airtight containers in dark, cool, dry place.
 Label containers with receiving, opening, and
disposal dates.
 Dispose of peroxide-forming chemicals before
expected date of first peroxide formation.
 Test for presence of peroxides periodically.
Chemical Classes:
__________
 Def: Dangerous or extremely dangerous to health
and life when inhaled, swallowed, or absorbed by
skin contact.
 Examples: phenol, arsenic, chloroform, nitric acid,
sulfuric acid, hydrogen peroxide, hydrochloric acid
 Storage Precautions:
 Store according to hazardous nature of chemical,
using appropriate security when necessary.
Turn to your partner – Where would you find the
information about how to store the chemical?
Find The Safety Equipment!
 Take a quick tour of your facility!
 Locate and record in your lab notebook the
location(s) of:
 Hazards in the Lab (biohazards or
chemical)
 Safety Equipment
 Chemical Storage Locations (look for
labeled, specialty cabinets and standard cabinets
with chemicals)
Chemical Hazards
Key issue in use, storage, and disposal is:
___________________
___________________!!
Thumbs Up-Thumbs Down
Is chemical compatibility information
on an MSDS?
Chemical Compatibility Chart
Closure
 Why are material safety data sheets required
in a facility?
 Take one minute to THINK, then WRITE for
one minute about why facilities require
MSDS, and why YOU as a technician should
read them often.
Homework
1. Complete the MSDS Activity Sheet using the
MSDS you found. If an electronic version is
used, you DO NOT need to print it, but you do
have to record your source website.
2. Identify at least 3 hazards in your facility.
Describe them, and determine ways to
prevent or minimize them.
Due tomorrow at the beginning of class.
References
 Roy, Ken; Safe Science Series
http://www.nsela.org/index.php?option=com_content&view=category&id
=71&Itemid=79 June 09, 2011.
 Lab Safety
 http://cfo.asu.edu/ehs-labsafety, June 9, 2011
 http://cfo.asu.edu/ehs-labsafety-references, June 9, 2011.
 Chemical Safety
 “Chemical Safety for Teachers and Their Supervisors- Grades 7-12”,
A publication of the American Chemical Society and the ACS Board-Council
Committee on Chemical Safety. Send email to oss@acs.org for a copy.
 MSDS
 http://www.flinnsci.com/search_MSDS.asp June 9, 2011
 http://www.fishersci.com/ Click MSDS Search June 10, 2011