2 cell types based on chromosome content
Gametes (haploid (n), 23 chromosomes) and Somatic Cells (Diploid (2n), 46 chromosomes)
Chromosome Type in Somatic Cells
Homologous Chromosomes
- highly similar in length and centromere position.
- have genes for the same trait in the same location (locus) but they may be different versions of that genetic trait (alleles). they're equivalent, not identical
Chromosome make up in somatic cells
- 23 chromosomes from ovum and 23 from sperm make up 46 pairs
- chromosomes 1-22 are autosomal (contain genes for traits)
- chromosome 23 contains genes for biological sex, it gets X from ovum and X or Y from sperm
The 2 stages of the somatic cell cycle
1. Interphase (G1, S, G2 phases) where growth, and prep happens
2. Mitotic (M phase) where mitosis happens
note: Cells that remain in the stage (i.e. don't divide again once mature) are said to be in G0 phase
What is the interphase G1 phase of somatic cell reproduction?
- G1 phase: growth, metabolism, centrosome replication begins
What is the interphase S phase of somatic cell reproduction?
- chromosomes replicate (but are still thread like and not individually visible)
- the replicates are called "sister chromatids" and attach at centromere
- this stage ALWAYS occurs before any cell division
- centrosome replication continues
What are kinetochores and what phase of interphase do they form?
proteins called kinetochores form on centromere to be site of attachment for spindle microtubules during mitosis and meiosis
What is the interphase G2 phase of somatic cell reproduction?
- Growth and metabolism
- production of enzymes & other proteins needed for cell division
- centrosome replication is completed
What is the interphase M phase of somatic cell reproduction?
- Where mitosis happens (Prophase, metaphase, anaphase, telophase)
- When this ends then mitosis and cytokinesis are complete, and resulting diploids enter G1 again and cycle restarts
Trick for remembering interphase stages?
- G1 and G2 are both Growth stages (and metabolism and replication)
- S phase, Synthesis, is doubling chromosomes
- M phase is Mitosis
What are the stages of somatic cell formation?
Interphase: G1, S, G2, M
then during M stage is mitosis: Prophase, metaphase, anaphase, telophase
What happens during prophase?
- chromosomes condense and becomes individually visible
- nucleoli disappear and nuclear envelope breaks up
- centrosomes move to opposite poles of cell
- spindle microtubules grow and attach to kinetochore proteins of each sister chromatid and begins to move them to center
What happens during Metaphase?
46 replicated chromosomes line up on cell equator
What phase of mitosis is this?
Prophase
What happens during Anaphase?
- spindle microtubules shorten and pull kinetochores away from each other, results in separation of sister chromatids to now have 92 individual chromosomes which migrate to each pole
- cytokinesis begins (division of cytoplasm)
What happens during telophase?
- chromosomes unravel once more to thread like strands
- nucleoli and nuclear envelope reappear
- spindle disassembles
- cytokinesis continues (or sometimes ends here)
What is the difference between Gamete formation and Somatic Cell formation?
Gamete: reproduces by meiosis, splits from one cell into 4 (makes sperm or ovum)
Somatic Cell: reproduces by mitosis, splits from one cell to 2
What are the stages of Meiosis?
Interphase stages (G1, S, G2, M) then Meiosis 1 (Prophase 1, Metaphase 1, Anaphase 1, telophase 1), then Meiosis 2 (Prophase 2, metaphase 2, anaphase 2, telophase 2)
When does cytokinesis begin and end in mitosis?
Begins in anaphase and ends after telophase
What happens during Meiosis 1?
Prophase 1: homologous chromosomes attach together and form tetrads
Metaphase 1: 23 tetrads line up along the cell equator
Anaphase 1: tetrads separate and a homologous chromosome goes to each pole
Telophase 1: chromosomes unravel once more to thread-like strands, nucleoli and nuclear envelope reappear, spindle disassembles, cytokinesis continues (or sometimes ends here)
What stage of meiosis is this?
Metephase 1
What state are the cells after meiosis 1 and cytokinesis?
- each cell has 23 chromosomes (haploid now)
- these cells do NOT have homologous chromosomes
- each cell has a copy of each autosomal chromosome and one sex one
- the two haploid cells enter interphase (G1)
What stage of Meiosis is this?
Telophase 1
What happens during Meiosis II?
Prophase II: sister chromatids attach together, nucleoli and nuclear envelope dissapear
Metaphase II: 23 chromosomes line up along the cell equator
Anaphase II: sister chromatids separate and a chromosome goes to each pole, cytokinesis starts
Telophase II: chromosomes unravel once more to thread-like strands, nucleoli and nuclear envelope reappear, spindle disassembles, cytokinesis continues (or sometimes ends here)
What process is this?
Meiosis II
What stage of Meiosis is this?
Prophase 1
Whats the point of Meiosis?
- Turn diploid germ cells into haploid gametes (ovum/sperm)
- 2 haploid gametes (ovum & sperm) will combine their 23 chromosomes to a zygote with 46 chromosomes
How many chromosomes line up at equator in mitosis and meiosis 1 & 2?
Mitosis: 46 chromosomes (sister chromatids)
Meiosis 1: 23 tetrads (homologous chromosomes)
Meiosis II: 23 chromosomes (sister chromatids)
State after cytokinesis in Meiosis 1?
2 haploid cells with nearly identical copies of 23 chromosomes with sister chromatids
State after cytokinesis in Meiosis II?
- Gametes now
- 4 haploid cells that each has a copy of 23 chromosomes (not sister chromatids)
Meiosis stages from interphase to cytokinesis?