Molecular biology techniques

2017-07-27T22:24:32+03:00[Europe/Moscow] en true Dansyl chloride, CRISPR, DNA footprinting, Diethylpyrocarbonate, DNA microarray, Green fluorescent protein, Plasmid, Dithiothreitol, Proteinase K, Cell culture, Restriction enzyme, Taq polymerase, X-gal, Coomassie Brilliant Blue, ChIP-on-chip flashcards Molecular biology techniques
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  • Dansyl chloride
    Dansyl chloride or 5-(DimethylAmino)Naphthalene-1-SulfonYL chloride is a reagent that reacts with primary amino groups in both aliphatic and aromatic amines to produce stable blue- or blue-green–fluorescent sulfonamide adducts.
  • CRISPR
    Clustered regularly interspaced short palindromic repeats (CRISPR, pronounced crisper) are segments of prokaryotic DNA containing short repetitions of base sequences.
  • DNA footprinting
    DNA footprinting is a method of investigating the sequence specificity of DNA-binding proteins in vitro.
  • Diethylpyrocarbonate
    Diethylpyrocarbonate (DEPC), also called diethyl dicarbonate (IUPAC name), is used in the laboratory to inactivate RNase enzymes in water and on laboratory utensils.
  • DNA microarray
    A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface.
  • Green fluorescent protein
    The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
  • Plasmid
    A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently.
  • Dithiothreitol
    Dithiothreitol (DTT) is the common name for a small-molecule redox reagent also known as Cleland's reagent.
  • Proteinase K
    In molecular biology Proteinase K (EC 3.4.21.64, protease K, endopeptidase K, Tritirachium alkaline proteinase, Tritirachium album serine proteinase, Tritirachium album proteinase K) is a broad-spectrum serine protease.
  • Cell culture
    Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment.
  • Restriction enzyme
    A restriction enzyme or restriction endonuclease is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites.
  • Taq polymerase
    Taq polymerase /ˌtæk ˈpɒlᵻməreɪz/ is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Chien et al.
  • X-gal
    X-gal (also abbreviated BCIG for 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is an organic compound consisting of galactose linked to a substituted indole.
  • Coomassie Brilliant Blue
    Coomassie Brilliant Blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry.
  • ChIP-on-chip
    ChIP-on-chip (also known as ChIP-chip) is a technology that combines chromatin immunoprecipitation ('ChIP') with DNA microarray ("chip").