2021-06-18T21:25:31+03:00[Europe/Moscow] en true How do microscopes give us a magnified image of an object?, How is the total magnification calculated?, Light rays, Seeing detailed objects, Electrons, Electron microscope, Electron microscope magnification flashcards

Microscopes

Comparing light and electron microscopes

  • How do microscopes give us a magnified image of an object?
    By using 2 lenses - they eye piece lens and the objective lens.
  • How is the total magnification calculated?
    How is the total magnification calculated?
    By multiplying both together. E.g: if the eye piece is x40 and the objective lens is x100 the total magnification is 4000.
  • Light rays
    Light rays have a long wave length (400-700nm). This is fine for studying human hair, which is about 100 times bigger than the wavelenght of light, but not for a bacterium that is 200nm in length or a protein molecule that is just 10nm.
  • Seeing detailed objects
    If you want to see in detail objects that are smaller than the wavelength of light, you need to use electrons.
  • Electrons
    Electrons have a very short wavelength (1nm) which allows us to see objects much smaller than the wavelenght of light.
  • Electron microscope
    In an electron microscope, a beam of electrons is used instead of light rays. When the electrons reach the specimen, some pass through and some bounce off - the electrons that pass through are detected and displayed as an image on a flourescent screen.
  • Electron microscope magnification
    Electron microspcopes have much higher magnification and resolving power (the ability to see 2 points as being separate) than a microscope. This means that cells can be studied in much finer detail and biologists can now see many more sub-cellular structures.