Measurement of growth
Direct measurement:
-direct microscopic count- counting chamber
-electronic counting (Coulter counter)
-plate count
-filtration
-most probable number method
Indirect measurement:
-turbidity
-metabolic activity
-dry weight/wet weight/cell volume
Counting chamber pro / cons
CFU - CFU/ml
Colony Forming Units - colonies / ml
Methods for plate counting
-Spread plate method
-Pour plate method
Plate count pro / cons
Filtration pro/ cons
Most probable number method
Principle of turbidity
Turbidity pros / cons
Growth control factors
Sterilisation
killing or removal of all viable microorganisms and viruses
Decontamination
treatment that renders an object or inanimate surface safe to handle
Disinfection
the elimination of pathogens from inanimate objects or surfaces
D-value
decimal reduction time: time in which 90%of the original population is killed