2024-09-26T04:42:04+03:00[Europe/Moscow] en true <p>Heat fixing</p>, <p>Alcohol fixing</p>, <p>it denatures the protein, and it can cause the milk to curdle or coagulate making it clumpy which makes it hard to view</p>, <p>kill the microorganisms, coagulate the protoplasm of the cell and cause it to adhere to the slide</p>, <p>simple staining </p>, <p>Methylene blue</p>, <p>Negative staining</p>, <p>transparent</p>, <p>Nigrosin</p>, <p>Differential staining</p>, <p>Methylene blue</p>, <p>Basic fuchsin</p>, <p>pink or red due to the basic fuchsin</p>, <p>dark blue or blue </p>, <p>Ammonium oxalate-crystal violet</p>, <p>Mordant</p>, <p>Iodine </p>, <p>95% ethyl alcohol </p>, <p>safranin</p>, <p>usually gram negative</p>, <p>Bacillus subtilis</p>, <p>Escherichia coli </p> flashcards

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  • Heat fixing

    slide is passed film-side up quickly through the flame of the alcohol lamp 2 to 3 times.

  • Alcohol fixing

    consists of covering smear with 95% ethyl alcohol for 1 minute, then draining the alcohol

  • it denatures the protein, and it can cause the milk to curdle or coagulate making it clumpy which makes it hard to view

    Why is heat fixing not suitable for preparations made up of milk

  • kill the microorganisms, coagulate the protoplasm of the cell and cause it to adhere to the slide

    purpose of fixation

  • simple staining

    only 1 dye used

  • Methylene blue

    dye used in teeth scraping

  • Negative staining

    dye does not adhere or stain the bacteria but rather the background

  • transparent

    color of bacteria in negative staining

  • Nigrosin

    dye used in negative staining

  • Differential staining

    usage of 2 or more dye

  • Methylene blue

    primary stain of differential staining

  • Basic fuchsin

    secondary/ counterstain of the primary stain of Differential staining

  • pink or red due to the basic fuchsin

    color of the cheek cells

  • dark blue or blue

    color of the diplococcus salivarious

  • Ammonium oxalate-crystal violet

    primary stain of gram staining

  • Mordant

    used to intensify the chemical bond between the primary stain of the cell wall of the microorganism

  • Iodine

    mordant used in gram staining

  • 95% ethyl alcohol

    decolorizer

  • safranin

    secondary stain

  • usually gram negative

    Plant pathogenic gram staining

  • Bacillus subtilis

    gram positive

  • Escherichia coli

    gram negative