Fertilization
a process not a single event; begins with sperm cell contact with egg, ends with the intermingling of maternal and paternal chromosomes at the metaphase plate prior to the first cleavage division
Internal Fertilization
a more efficient mechanism of fertilization; characteristic of mammals
Components of Fertilization
initial membrane contact between sperm and egg
entry of sperm into the egg
prevention of polyspermy
metabolic activation of the egg
completion of meiosis by the egg
formation and fusion of male/female pronuclei -leading to the first cleavage division
Polyspermy
entry of more than one sperm into the egg cell
Sea urchin eggs
at cellular level, they are covered by a noncellular jelly coat, and the eggs complete their second meiotic division before the entry of spermatozoon
Mammals (internal fertilization)
eggs are surrounded by a cellular layer (not a jelly coat) and must complete the second meiotic devisionjelly coat,
Fishes
Female extrudes egg
Male floods area with spermatozoa
Jelly coat (sea urchins)
thick outer investment; is composed of a mixture of small peptides, glycoproteins, and a polysaccharide containing fucose sulfate units.
Acrosome reaction
Direct contact with jelly coat increases motility and stimulates the ____
Speract
a decapeptide (10 amino acids), in one species of sea urchin, that is responsible, at least in part, for the increased motility and activated respiration that occur when the sperm contacts jelly coat
Consequence of the interaction of the sperm with speract
an increase in permeability of the plasma membrane of the sperm to Ca++ and an increased concentration of intracellular Ca++ and a subsequent exchange of inside the sperm head for extracellular Na+.
Acrosome reaction
stimulated by an exchange of extracellular Ca++ with intracellular K+, and if the intracellular pH is higher than 7.2, it begins with a localized fusion of the outer acrosomal membrane with the plasma membrane and their ultimate breakdown
Consequences of acrosome reaction
the release of the soluble enzymes located within the acrosomal vesicle.
polymerization of g-actin (the globular form) within the subacrosomal region tof-actin (the filamentous form).
Bindin
protein that mediates sperm binding to the surface of the egg
tip of the acrosomal process is covered with this protein
Vitelline envelope
a tough noncellular layer interposed between the jelly coat and the plasma membrane of the egg
composed of glycoprotein and polysaccharide molecules
Lysins
acrosomal enzymes with trypsinlike activity
Plasma membrane of egg
After making its way through the vitelline coat, a sperm cell makes contact with the ______
Intracellular and extracellular region
The sperm receptor molecule consists of an _____
Extracellular region
This region differs from species to species and is responsible for ensuing that only sperm of the same species fertilizes the egg
Intracellular region
This region remains constant among different species, and it may serve to stimulate a signal transduction event that initiates the profound changes that affect the egg immediately after sperm-egg membrane fusion
Membrane fusion
a common feature of fertilization in all animals
first reported by Colwin and Colwin (1963)
Fertilization cone
Soon after sperm-egg fusion, a group of microvilli seem to engulf the head of the sperm, causing a bulge known as the ____
Where the sperm has made contact with the egg, the cytoplasm of the egg in many species bulges out in an elevated process called the_______
The fast block to polyspermy
a membrane event that is set in place within 2 to 3 seconds and lasts for about 60 seconds, by which time the permanent (slow) block to polyspermy is established
Resting membrane potential
difference in electrical potential generated by the plasma membrane of the egg
Cortical reaction
the rupture of cortical granules and the release of these contents into the space surrounding the egg (the perivitelline space)
Fertilization membrane
new name given to the vitelline envelope after it has undergone the changes set in motion by the cortical reaction
Hyaline layer
located between the plasma membrane and fertilization membrane
Hydrogen peroxide (H2O2)
powerful oxidizing agent
released by the egg at the time ofthe cortical reaction
Ovoperoxidase
mediates the chemical breakdown of H2O2 at the fertilization membrane
Blocks to polyspermy
an effective way of maintaining the genetic integrity of the fertilized egg
Urodele amphibians and birds
In these species, polyspermy is normal and other means have been devised for inactivating and removing excess sperm from the fertilized egg
Sperm main function (early stages of fertilization)
activate a program of events that is already patterned in the egg
Activation of the egg
begins with the influx of Na+ associated with the membrane depolarization of the fast block to polyspermy.
Release of intracellular Ca2+
appears to be the main stimulus for the next major series of events (in activation of egg).
Enzyme NAD kinase
facilitate the biosynthesis of new membrane lipids
True
As the sperm nucleus becomes incorporated into the fertilization cone of the egg, the nuclear membrane begins to disintegrate.
Male pronucleus
As the phase of chromatin dispersion nears completion, a new membrane forms around what can now be properly called the ______
Sperm aster
a radiating array of microtubules emanating from the original centrioles of the sperm
important in getting the male and female pronuclei together
plays a major role in guiding the migrations of the pronuclei
Pronuclear fusion
As the two pronuclei (male/female) come into contact with each other, their membranes fuse, leading to both maternal and paternal chromosome being enclosed in a single membrane.
Replicate their DNA
Shortly after pronuclear fusion, the chromosomes _______ in preparation for the first cleavage division.
Natural acidity of the upper vagina
The first barrier the spermatozoa has the face
Natural acidity of the upper vagina
The apparent function of this acidity is to act as a bacteriostatic medium.
Seminal fluid
acts as an effective buffer against natural vaginal acidity
Rodents
In ______, the semen coagulates shortly after insemination and forms a characteristic plug which prevents the backflow of spermatozoa
The entrance into the uterine tubes
The next barrier in the path of the spermatozoa after the natural acidity of the upper vagina
Uterotubal junction
may act as a valve which permits or prevents the passage of spermatozoa into the uterine tube
Positive rheotactic response
The spermatozoan response in which they orient themselves so that they move against a gentle current
Capacitation
As the spermatozoa are transported through the female reproductive tract, they are subjected to a poorly understood influence by the maternal tissues which enables them better to penetrate the membranes surrounding the egg. This phenomenon is called _______
True
The spermatozoa that are not directly involved in fertilization are ultimately removed from the female reproductive tract. Those in the uterine cavity are eventually swept through the cervix and into the vagina; those in the uterine tubes are ingested by phagocytic cells.
immotile cilia syndrome
women who have the ________ are typically fertile
Corona radiata
____(cells of the cumulus oophorus) surrounding the ovum is very important in egg transport
without this layer, ovum makes little progress
Aging or Deterioration
certain changes the ovum begins to undergo when liberated from the ovary
True
Motility lasts much longer than the ability to fertilize
Mammals
fertilization occurs in the upper part of the uterine tubes
True
The spermatozoa must first penetrate the cells of the corona radiata and then the zona pellucida before they can make contact with the plasma membrane of the egg
Hyaluronidase
a sperm-derived enzyme, that facilitates penetration through the corona radiata by dissolving extracellular matrix material around the cells.
Sperm receptors
When the spermatozoa reach the zona pellucida, specific molecules on the head of the sperm bind to species-specific ______, which consist of exposed parts of the ZP-3 molecule.
Acrosome reaction
Further contact with the sperm head and other core regions of the ZP-3 molecule induce the _________
Capacitation is a prerequisite for this reaction
lytic enzymes
enzymes that facilitate the passage of the sperm through the zona pellucida
First step in acrosome reaction
The localized fusion of portions of the outer acrosomal membrane with the overlying plasma membrane of the spermatozoon.
Zona pellucida
After the acrosome reaction, the spermatozoon digests a narrow pathway through the ______
Acrosin
a trypsinlike proteinase responsible for the spermatozoon's digestion of a narrow pathway through the zona pellucida
bound to the inner acrosomal membrane, which after the completion of the acrosome reaction is exposed to the surface of the head of the sperm
Fluid-filled perivitelline space
Once through the zona pellucida, the spermatozoon enters the _____________ between the zona and the plasma membrane of the ovum.
Acrosome reaction
This reaction appears to cause a change in the plasma membrane of the sperm that allows it to fuse with other cell membranes
Microvilli
Contact is quickly established between the egg and sperm membranes and may be faciliated by the ______ projecting from the ovum.
True
As in the sea urchin, it is important for the egg to prevent polyspermy once it has been penetrated by the first sperm cell. This is accomplished by means of blocks to polyspermy
True
With sperm entry, the block to the second meiotic division of the egg is rapidly lifted and the second polar body is released, leaving a haploid female nucleus.
True
after entry into the egg, the nuclear membrane of the sperm breaks down, allowing the interaction between the nuclear contents of the sperm and the cytoplasm of the egg.
Protamines
facilitate the extremely dense condensation of the nuclear chromatin that is required for proper packing of the nucleus of the mature sperm cell.
Decondensation
_______ ofthe sperm head is more complex than a mere exchange of protamines for histones
In the early stages of ______, the enzymatic breakdown of numerous disulfide bonds that keep the sperm chromatin condensed also occurs.
Parthenogenesis
unfertilized eggs may become activated and develop into viable individuals as part of the normal life cycle
a reproductive strategy that involves development of a female gamete without fertilization
False
The sex of most animals is not determined at the time of fertilization
True
In mammals, the egg contributes an X chromosome to the process, whereas the sperm will contribute either an X or a Y chromosome, thus determining the sex of the zygote (XX for female and XY for male).
Birds
In this group of vertebrate, the chromosomal pairs of the male are alike, and those of the female show sexual differences.
Sexual differentiation
The union of gametes and the genetic determination of sex constitute just the beginning of a long process of ________
Sex chromatin or Barr body
chromatin mass
Sex chromatin
represents the morphological expression of a genetic control mechanism
True
The activity of only one X chromosome is required or permissible for normal development in either males or females.
True
(both X chromosomes are actively functioning during early cleavage, but as the trophectoderm and later the primitive endoderm form during the early blastocyst stage)
Sex chromatin bodies are typically not seen during early embryonic cleavage
True
the paternal X chromosome is selectively inactivated in each of these extraembryonic tissues
True
The vertebrate body is bilaterally symmetrical
craniocaudal (anteroposterior) axis
dorsoventral axis
mediolateral axis
Vertebrate body three polar axes
Animal pole
The nucleus (germinal vesicle) is located near the ______
Vegetal pole
Both the size and the concentration of yolk platelets increase markedly toward the ______
Amphibians
In this group of vertebrate, the region of the animal pole will ultimately form the head and the vegetal pole will form the tail
Keys to fixation of the dorsoventral axis
(1) the activation of a specific region of vegetal cytoplasm and its displacement toward the cortex of the vegetal pole
(2) as cleavage occurs, the inclusion of the activated vegetal cytoplasm in cells (blastomeres) located near the gray crescent (future dorsal midline) area
(3) an inductive action of these cells on the neighboring cells in the equatorial region (between the animal and vegetal hemispheres)
dorsalizing blastomeres
sometimes called the dorsalizing center or Nieuwkoop center
dorsoventral
the _________ axis can be predicted by the position of cells or cell layers relative to the yolk
craniocaudal axis
________ becomes fixed after the fertilized egg has been in the uterus from 14 to 16 hours.