2017-07-27T22:09:37+03:00[Europe/Moscow] en true Phenylthiocarbamide, Carboxyfluorescein succinimidyl ester, Gel electrophoresis, Reverse transcriptase, Transformation (genetics), 2-Mercaptoethanol, Dissection, Biomarker, Ethidium bromide, Morpholino, Fluorescein, Avidin, X-ray spectroscopy, DNA methyltransferase, Molecular-weight size marker flashcards
Biological techniques and tools

Biological techniques and tools

  • Phenylthiocarbamide
    Phenylthiocarbamide (PTC), also known as phenylthiourea (PTU), is an organosulfur thiourea containing a phenyl ring.
  • Carboxyfluorescein succinimidyl ester
    Carboxyfluorescein succinimidyl ester (CFSE) is a fluorescent cell staining dye.
  • Gel electrophoresis
    Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
  • Reverse transcriptase
    A reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.
  • Transformation (genetics)
    In molecular biology, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s).
  • 2-Mercaptoethanol
    2-Mercaptoethanol (also β-mercaptoethanol, BME, 2BME, 2-ME or β-met) is the chemical compound with the formula HOCH2CH2SH.
  • Dissection
    Dissection (from Latin dissecare "to cut to pieces"; also called anatomization), is the dismembering of the body of a deceased animal or plant to study its anatomical structure.
  • Biomarker
    A biomarker, or biological marker, generally refers to a measurable indicator of some biological state or condition.
  • Ethidium bromide
    Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
  • Morpholino
    A morpholino, also known as a morpholino oligomer and as a phosphorodiamidate morpholino oligomer (PMO), is type of oligomer molecule (colloquially, an oligo) used in molecular biology to modify gene expression.
  • Fluorescein
    Fluorescein (/flɔərˈɛsiᵻn/ or /flɔərˈɛsin/) is a synthetic organic compound available as a dark orange/red powder slightly soluble in water and alcohol.
  • Avidin
    Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.
  • X-ray spectroscopy
    X-ray spectroscopy is a gathering name for several spectroscopic techniques for characterization of materials by using x-ray excitation.
  • DNA methyltransferase
    In biochemistry, the DNA methyltransferase (DNA MTase) family of enzymes catalyze the transfer of a methyl group to DNA.
  • Molecular-weight size marker
    A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix.