2022-11-01T02:14:53+03:00[Europe/Moscow] en true <p>What is glycosylation?</p>, <p>What are the two types of glycosylation</p>, <p>How is the the N-linked sugar added?</p>, <p>What happens after the transfer of the N-linked sugar?</p>, <p>What are the three different Golgi sacs/cisternae?</p>, <p>Why do we have glycosylation?</p> flashcards

Bio230 week 6 part 2

Protein Sorting- Glycosylation and Vesicles

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  • What is glycosylation?

    Adding sugar chains to proteins, most soluble and transmembrane proteins in ER are glycosylated

  • What are the two types of glycosylation

    O-Linked - adding sugar to oxygen, oxygen is on side chain (10%)N-Linked - adding sugar to nitrogen, nitrogen on asparagine (Asn) side chain (90%)

    O-Linked - adding sugar to oxygen, oxygen is on side chain (10%)

    N-Linked - adding sugar to nitrogen, nitrogen on asparagine (Asn) side chain (90%)

  • How is the the N-linked sugar added?

    It is transferred by an oligosaccharyl transferase to an Asn on a protein being synthesized. The transferase looks for Asn-X-Ser or Asn-X-Thr where X is any amino acid besides proline. Proteins are only glycosylated on the ER lumen side.

  • What happens after the transfer of the N-linked sugar?

    1. 3 glucoses are removed (linked to proper folding of protein)2. 1 Mannose is removed3. Glycosylated protein is transported via vesicles to the Golgi4. That glycosylated protein is further modified in Golgi sacs

    1. 3 glucoses are removed (linked to proper folding of protein)

    2. 1 Mannose is removed

    3. Glycosylated protein is transported via vesicles to the Golgi

    4. That glycosylated protein is further modified in Golgi sacs

  • What are the three different Golgi sacs/cisternae?

    Cis, Medial, and Trans

  • Why do we have glycosylation?

    Tag to mark the state of protein folding

    Protect proteins on cell surface from proteases

    Play a role in cell adhesion

    Allow proteins to form correct structures