definition of magnification
how many times bigger the image of a specimen observed is in compared to the actual size of the specimen
two type of lens of a light microscope
eyepiece lens
objective lens
magnification of an eyepiece lens
x10
formula to calculate total magnification
eyepiece lens magnification x objective lens magnification
definition of resolution
the ability to distinguish between two separate points
limitation of the light microscope
the wavelength of visible light
light passing through a specimen will be
diffracted
the longer the wavelength of light, the
more it is diffracted
electron compared to light microscopes
higher resolution and magnification
reason for difference in resolution and magnification in microscopes
electrons have a much smaller wavelength than visible light
maximum resolution of a light microscope
200nm
reason the phospholipid bilayer cannot be observed under a light microscope
width of phospholipid bilayer is 10nm
maximum resolution of a light microscope is 200nm
visible light wavelength
400 - 700nm
wavelength of a beam of electrons
1nm
type of microscope used to view live or dead specimens
light microscopes
examples of light microscope specimens
whole cells
small plant or animal organisms
tissues within organs
in electron microscopes, a broad static beam is used for
transmission
in electron microscopes, a small beam that moves across the specimen is used for
scanning
type of lens used in an electron microscope
electromagnetic
examples of specimens viewed by an electron microscope
organelles, viruses and DNA
whole cells in detail
an electron microscope specimen must be
dead
which microscope specimen requires complicated sample preparation
electron microscope
the portable type of microscope
light microscope
which microscope requires a vacuum
electron microscope