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AS Level Resolution and Magnification

CIE Syllabus 1.1.4

  • definition of magnification

    how many times bigger the image of a specimen observed is in compared to the actual size of the specimen

  • two type of lens of a light microscope

    eyepiece lens

    objective lens

  • magnification of an eyepiece lens

    x10

  • formula to calculate total magnification

    eyepiece lens magnification x objective lens magnification

  • definition of resolution

    the ability to distinguish between two separate points

  • limitation of the light microscope

    the wavelength of visible light

  • light passing through a specimen will be

    diffracted

  • the longer the wavelength of light, the

    more it is diffracted

  • electron compared to light microscopes

    higher resolution and magnification

  • reason for difference in resolution and magnification in microscopes

    electrons have a much smaller wavelength than visible light

  • maximum resolution of a light microscope

    200nm

  • reason the phospholipid bilayer cannot be observed under a light microscope

    width of phospholipid bilayer is 10nm

    maximum resolution of a light microscope is 200nm

  • visible light wavelength

    400 - 700nm

  • wavelength of a beam of electrons

    1nm

  • type of microscope used to view live or dead specimens

    light microscopes

  • examples of light microscope specimens

    whole cells

    small plant or animal organisms

    tissues within organs

  • in electron microscopes, a broad static beam is used for

    transmission

  • in electron microscopes, a small beam that moves across the specimen is used for

    scanning

  • type of lens used in an electron microscope

    electromagnetic

  • examples of specimens viewed by an electron microscope

    organelles, viruses and DNA

    whole cells in detail

  • an electron microscope specimen must be

    dead

  • which microscope specimen requires complicated sample preparation

    electron microscope

  • the portable type of microscope

    light microscope

  • which microscope requires a vacuum

    electron microscope