A - a.a. attaches to tRNA @ 3' end, forms aminoacyl-tRNA(AT) catalysed by AT synthetase; AC determines a.a. tRNA binds to

I - tRNA w/ Met a.a, AC UAC, binds to SRS w/ aid of IF. SRS r&b 5' end mRNA, moves until reaches AUG. AC UAC CBPs w/ AUG on mRNA; initiator tRNA+SRS+mRNA followed by attachment of LRS, forms TIC; GTP brings components together. initiator tRNA fits into P site of LRS, A site ready for next AT.

E - AC of next AT w/ specific a.a. CBPs & forms H bonds w/ mRNA codon @ A site of ribosome. PT bond formed b/w N-end of a.a. @ A site & C-end of ppt @ P site, catalysed by PT. ribosome moves 1 codon down mRNA 5'->3', moves tRNA carrying ppt from A->P site, tRNA in P site->E site, leaves ribosome, A site free for next AT

T - stop codon reaches A site, PRF r&b to stop codon, H₂O added to ppt, hydrolyses ppt from tRNA in P site, releases ppt from tRNA & ribosome. TIC disassembles

Learn: H2 Biology 8 - DNA Replication(ii)