Objective: How does DNA determine the proteins that get synthesized? Take the page and divide it into 6 equal boxes Number the boxes Notes: Protein Synthesis 1 2 3 4 5 6 Protein Synthesis “making a protein” Step 1. Transcription Step 2. Translation TALK IT OVER: Look to the picture at the model of DNA and RNA, what do you notice different about RNA? Single strand Has sugar ribose Has nitrogen bases: Adenine (A), Cytosine (C), Guanine (G), Uracil (U) Messenger RNA (mRNA) – Carries the genetic information to make the protein Ribosomal RNA (rRNA) – makes up ribosomes Transfer RNA (tRNA) – carries amino acids to the ribosome Transcription –DNA is copied into mRNA Happens in the nucleus DNA Sequence: A T G C RNA Sequence: U A C G Grab a white board and maker. Time for some practice. Given the following sequence of DNA; transcribe the mRNA that would get made. G C A A C T T A C C G U U G A A U G 1. Give the complementary strand: A C T G C A T T T T G A C G T A A A 2. Transcribe the following DNA strand: G C C C A T A A A C G G G U A U U U Translation: mRNA is made into protein mRNA read 3 bases at a time = Codon = 1 amino acid Different order in amino acids = different proteins = different traits Without looking at your SNB, 1. What nitrogen base is found only in RNA 2. 3. 4. Uracil What is molecule is getting made during transcription? mRNA If there are 15 bases, how many amino acids would it could for? 5 amino acids What molecule is getting made during translation? Amino acid/protein Grab a white board and maker. Time for some practice. Given the following sequence of mRNA; translate the mRNA that would get made. G C A A C U U U C Ala Phe Thr Given the following DNA sequence, first transcribe, than translate it: TAC CTT GCC AUG GAA CGG Met Glu Arg Answer the following questions within this box: How are proteins made from DNA? Answer in 2-3 complete sentences Highlight and # the periods Use the following words: DNA, mRNA, transcription and translation