ANIMAL DEVELOPMENT 1 CH. 47 MECHANISMS OF MORPHOGENESIS AND CELL FATE MECHANISMS OF MORPHOGENESIS Cell movement in Morphogenesis Only animals experience cell movement Cytoskeleton plays a large role 2 cells crawl within embryo using cytoskeletal fibers to extend and retract cellular protrusions Like amoeboid movement Cell adhesion molecules and ECM are involved Ectoderm Neural plate Microtubules Actin filaments Neural tube 3 FIGURE 47.15-5 4 FIGURE 47.16 MECHANISMS OF MORPHOGENESIS Apoptosis- programed cell death example: tails cells in humans 5 example: inner digit cells CELL FATE SPECIFICATION Determination- cell or group of cells become committed to a particular fate Differentiation is the resulting specialization All cells have the same genes just a matter of gene expression HHMI Embryonic Stem Cells and Cell Fate 6 http://www.hhmi.org/biointeractive/creating-embryonic-stemcell-lines CELL FATE SPECIFICATION 7 Fate maps- diagrams showing the structures from each region of the embryo Epidermis Central nervous system Notochord Epidermis Mesoderm Endoderm Blastula Neural tube stage (transverse section) (a) Fate map of a frog embryo 64-cell embryos Blastomeres injected with dye Larvae (b) Cell lineage analysis in a tunicate 8 FIGURE 47.17 CELL FATE SPECIFICATION Fate maps- diagrams showing the structures from each region of the embryo Example axis formation bilateral symmetry gray crescent is the future dorsal side (opposite sperm entry) 9 different genes are expressed because different aprts are exposed to different environment FIGURE 47.21 Dorsal Right Anterior Posterior Left Ventral (a) The three axes of the fully developed embryo Animal pole Vegetal hemisphere Vegetal pole (b) Establishing the axes Point of sperm nucleus entry Pigmented cortex Future dorsal side Gray crescent 10 Animal hemisphere First cleavage FIGURE 47.22-2 EXPERIMENT Control egg (dorsal view) Experimental egg (side view) 1a Control 1b Experimental group group Gray crescent Gray crescent Thread 2 Normal Belly piece Normal 11 RESULTS CAN CELL FATE BE MODIFIED? Development potential- what it can become First two cells are totipotent- can become a new organism Mammals are totipotent to 8 cells 16 cells to trophoblast or (inner cell mass) cells are not totipotent but nuclei are 12 These cells would be pluripotent; can become almost any cell (can’t become the placenta) 13 CELL FATE INDUCTION “Organizer” inactivate BM4 (bone morphogenic protein) on dorsal side 14 Positional information and pattern formation relate to molecular signaling FORMATION OF VERTEBRATE LIMBS Apical ectodermal ridge (AER) regulates limb bud development by secreting proteins that signal fibroblast growth factor (FGF). Zone of polarizing activity (ZPA) regulates limb buds development by secreting a protein growth factor Sonic Hedgehog. Cells nearest the ZPA give rise to posterior structures. 15 Hox genes determine if front or hind limbs FIGURE 47.24 Anterior Limb bud AER ZPA Posterior Limb buds 50 m 2 Digits Apical ectodermal ridge (AER) Anterior 3 4 Ventral Proximal Distal Dorsal Posterior (b) Wing of chick embryo 16 (a) Organizer regions EXPERIMENT Anterior New ZPA Donor limb bud Host limb bud ZPA Posterior RESULTS 4 3 2 2 4 3 17 FIGURE 47.25 FORMATION OF VERTEBRATE LIMBS Sonic hedgehog is a ligand (protein) that diffuses to form a concentration gradient and has different effects on cells of the developing embryo depending on its concentration. 18 SSH remains important in the adult. It controls cell division of adult stem cells and has been implicated in development of some cancers. CILIA AND CELL FATE Monocilia Stationary single projections on nearly all animal cells 19 A19cts as antenna on cell surface to receive signals from multiple proteins MOST IMPORTANT TO REMEMBER 20 PRODUCTS OF GENES ALLOW CELLS TO SPECIALIZE