EUKARYOTIC CELL ORGANELLES AND STRUCTURES Cytoplasm: • The cytoplasm is the cellular region between the nuclear membrane and the plasma membrane; It consists of a fluid called cytosol (which contains dissolved solutes, stores nutrients, secretory products and pigment granules) and organelles-the metabolic machinery of the cytoplasm and the cell. Function: Provides moisture and nutrition. Nucleus: • Function: It is the control center of the cell; responsible for transmitting genetic information and providing the instructions for protein synthesis. • The nucleus contains chromatin-granular, threadlike material composed of DNA and histone proteins both of which make up genes. When a cell is ready to divide, chromatin coils, condenses, and becomes chromosomes. Nuclear Envelope • The Nuclear envelope is a double-membrane structure surrounding the nucleus. It is pierced by the pores and the outer membrane is continuous with the cytoplasmic endoplasmic reticulum. • It functions to separate the nucleoplasm from the cytoplasm and regulates passage of substances to and from the nucleus. • The Nucleolus is a dense, spherical (nonmembrane bound) body made of ribosomal RNA and proteins: functions in making ribosomes. A single nucleus has one or more nucleoli. Ribosomes: • Ribosomes are non-membranous organelles. Function: to make proteins; ribosomes are free in the cytoplasm or bound to rough ER (endoplasmic reticulum). Proteins to be used within the cytosol are produced by the free - floating ribosomes; proteins to be inserted into membranes or exported from the cell are made by the ribosomes attached to the rough ER. Endoplasmic reticulum: • It is a network of canals extending from the plasma membrane to the nucleus. Function: to transport proteins out of the cells. Two types: Rough ER-has ribosomes attached to it, and functions to attach polysaccharide groups to proteins as they are made by the ribosomes to be exported from the cell; Smooth ER-has no ribosomes attached; and functions to make lipids and hormones (steroids) to be exported from the cell, or sometimes used by that cell. Golgi apparatus: • Is a group of flattened sacs arranged like a stack of bowls; functions to modify and package proteins and lipids into vesiclessmall, spherically shaped sacs that bud from the outside surface of the Golgi body. After modifications are done, these are shipped outside the cell. vesicles-membrane bodies with many functionsusually storage or transportation • Lysosomes: vesicles from a Golgi apparatus that contains digestive enzymes. Function to break down food, cellular debris, and foreign invaders such as bacteria. Not present in plant cells. • Peroxisomes: sacs in the cytoplasm that contain powerful oxidase enzymes. Function:to use molecular oxygen to detoxify harmful substances by changing it to water. Common in liver and kidney cells; also in photosynthesizing plant cells. Mitochondria: • look like kidney beans or linked sausages; these are the powerhouse of the cell; function as sites of aerobic respirationwhere energy in the form of ATP is obtained from carbohydrates. • Most numerous in cells having a high energy requirement(muscle cells, or liver cells). Each contains an inner membrane and outer membrane. The inner membrane has many long folds called cristae-these enlarge the surface area of the inner membrane, allowing more space for chemical reactions to occur during times of great energy production. The outer membrane serves as a boundary between the mitochondrion and the cytosol. • ****Mitochondria also have their own DNA-this is unique. New mitochondria can only arise when existing ones grow and divide. Theory is mitochondria developed from prokaryotic cells that lived inside eukaryotic cells-????? • Cell Fibers-microtubules, intermediate filaments, and microfilaments-all function in making the shape of or in coordinating the movements of the cytoskeleton-internal scaffolding of the cell within the cytoplasm. • Flagella-long, hair-like structure-whips for movement (are the tails on sperm) • Cilia-hundreds of tiny, hair-like structureswhip for movement (in mucosa areasbronchial tubes, nasal passages, etc) • Microvilli-fingerlike extensions of the plasma membrane used to increase surface area. (intestines-absorb food). • Centrioles-two dots that form near nucleus during cell division-will make a microtubule spindle in animal cells during mitosis (located in centrosomes-areas containing centrioles) In Plants: • Chloroplasts-carry out photosynthesis • Plastids – store nutrients and pigment molecules • Cell walls-made of cellulose or chitinprovide support for cell. (found in plants, fungi, protists, bacteria). • (Large)Vacuole -membrane bodies usually used for storage