Names of Authors: Thao Le Thanh, KumarasamyMurugesan*, In-Hyun Nam, Jong-Rok Jeon,
Yoon-Seok Chang*
Address: School of Environmental Science and Engineering, Pohang University of
Science and Technology (POSTECH), Pohang 790-784, Republic of
Korea
* Corresponding author
Prof. Yoon-Seok Chang
School of Environmental Science and Engineering,
Pohang University of Science and Technology (POSTECH), Pohang 790-784,
Republic of Korea
Tel.: +82 54 279 2281 ; Fax: +82 54 279 8299
E-mail address: yschang@postech.ac.kr (Y.-S. Chang)

Table S1. Sequences of Primer sets
Name of gene Sequence Reference dnxA dxnF (5’-TGYASNTAYCAYGGVTGG-3’) dxnR (5’-TBVGGNCCVYKNGGVTGCC-3’)
Iwai et al.
2011 bphA bphA-F (5'-TTCACCTGCASCTAYCACGGC-3') bphA-R (5'-ACCCAGTTYTCDCCRTCGTCCTGC-3') c23o
Uhlik et al.
2009
C23O-F (5’-AAGAGGCATGGGGGCGCACCGGTTCGATCA-3’) Lillis et al.
C23O-R (5’-AACAAA(AGT)GCGC(GC)GTCATGCGG-3’) 2000
Iwai, S., Johnson, T.A., Chai, B., Hashsham, S.A., Tiedje, J.M. (2011) Comparison of the
Specificities and Efficacies of Primersfor Aromatic Dioxygenase Gene Analysis ofEnvironmental Samples.Appl Environ Microbiol 77, 3551–3557.
Lillis, L., Clipson, N., and Doyle, E.(2000) Quantifcation of catechol dioxygenase gene expression in soil during degradation of 2,4-dichlorophenol. FEMS Microbiol Ecol 73 , 363-369.
Uhlik, O., Jecna, K., Mackova, M., Vlcek, C., Hroudova, M., Demnerova, K., Paces, V., Macek,
T. (2009) Biphenyl-metabolizing bacteria in the rhizosphere of horseradish and bulk soil contaminated by polychlorinated biphenyls as revealed by stable isotope probing. Appl Environ
Microbiol 75 ,6471-6477.

Figure. S1 . Growth of Agrobacterium sp. PH-08 in binary substrates and color changes during the growth

Figure. S2 . Phylogenetic tree of Agrobacterium sp. PH-08 (JN862809) in comparison to
Porphyrobactersanguineus (AB062106) and Porphyrobactersanguineus IAM 12620.

Figure. S3 . LC-chromatogram with retention time of some metabolites of DF degradation by
Agrobacterium sp.
PH-08 after 4 days incubation, including: Gentisic acid (1.289), Salicylic acid
(1.441).

Figure. S4. Identification intermediates of biodegradation of DF+BP dual substrates by
Agrobacterium sp.
PH-08. MS-chromatogram of dual substrates degradation.

Figure. S5. Identification intermediates of biodegradation of DF+DBT dual substrates by
Agrobacterium sp.
PH-08. GC-chromatogram of dual substrates degradation ( a ) and Mass spectra of metabolites ( b )
(a)
(b)

Figure. S6. Oxidation of catechol by Agrobacterium sp. PH-08. UV-VIS spectra indicating the
PH-08 strain exhibited meta-cleavage by catechol-2,3-dioxygenase and the enzyme activities were measured with 18.67 U/mg (PH-08).
(a)
(b)

Figure. S7. PCR amplified dxnA gene ( a ) and RT-PCR product of catechol-2,3-dioxygenase gene of total genomic DNA and total RNA ( b )from strain PH-08.
(a) (b)