INTRODUCTION TO THE LIVING WORLD KINGDOM MONERA KINGDOM PROTISTA LECT # 1 Introduce the organization of the living world • The algae (singular, alga) are generally considered simple plant-like organisms, both morphologically and anatomically. This is a reflection of the stable, aquatic environment, which they inhabit. Algae are found in waters of varying salinity, including both fresh water and marine environments. Although quite simple in form, the algae are an extremely diverse group. They vary in size from the minute (1-5 um) to the very large species such as kelp, known to grow to 50 m or more in length. Taxonomists suggest there are probably over 100,000 different algal species. This diversity has occurred over 2 to 3 billion years. Introduce the organization of the living world • These differences are mainly morphological, rather than anatomical, and are a result of changes in cell differentiation and variations in planes of cell division. Expressed in a different way, differences in algae are due to differences in shapes of cells in single celled algae; number of cells in multicellular forms and differences in the planes of cell division. The Algal classification systems can vary. Algal divisions have been established using several criteria including pigmentation differences, type of energy storage products, cell wall composition and the presence or absence of flagella (as well as the number and placement of these flagella, when present). INTRODUCTION TO CLASSIFICATION OF ORGANISMS • In order to systematically study the diverse organisms on earth, scientists had to organize the vast amount of different species of organisms. Taxonomy (Greek, "organizing rules") is the science of naming, describing and classifying the organisms into similar groups. The formal classification scheme in use today consists of a series of 7 major categories or taxa (singular, taxon). We place organisms in these taxa based on their similarities, then group similar taxa into larger and larger groupings. The basic structure of classification currently used is as follows: • KINGDOM PHYLUM (DIVISION) • CLASS • ORDER • FAMILY • GENUS • SPECIES • (A good acronym for remembering the above order of classification is: "King Phillip Came Over For Good Spaghetti") INTRODUCTION TO CLASSIFICATION OF ORGANISMS • Once plants and animals are classified and assigned to major taxa, each kind of organism needs to be identified by a name. We use a binomial system of nomenclature based upon the scheme originally introduced by Carolus Linnaeus, in the 18th century. In this system, each organism is identified by a universally understood two-part Latin or Latinized name consisting of the name of the genus and the species to which the organism belongs, so that the rat is always Rattus rattus and the earthworm is always Lumbricus terrestris whether it is being studied in Japan, Egypt, or Canada. The genus name is always capitalized while the species name is not. Both are underlined or italicized. The two words together are called the species name or scientific name of an organism. INTRODUCTION TO CLASSIFICATION OF ORGANISMS • In this course, you will also note that the spelling of certain biology words changes depending on whether they are used in a singular or plural form, for example: bacterium (singular) and bacteria (plural). Some other examples are: spirillum (spirilla), ovum (ova) • Most biologists divide the living world into five "Kingdoms". These Kingdoms are: • 1. Kingdom Monera • 2. Kingdom Protista • 3. Kingdom Fungi • 4. Kingdom Plantae • 5. Kingdom Animalia Unicellular Algae • These are the simplest algae. Except for a few that live in damp terrestrial habitats, these organisms are mainly confined to aquatic environments which provide an adequate supply of nutrients, water, a means of dispersal and a degree of support. Thus, these simple organisms can survive and even thrive in this environment. Although they consist of a single cell, botanists are able to place them in literally thousands of different genera and species. The range of cellular morphology is, at least in part, an adaptation for floatation or for predation avoidance. These organisms must have some ability to float at least through a portion of their life cycle because they must remain near enough to the surface to obtain sufficient light for photosynthesis. Large irregular shaped cells have the advantage of being avoided by herbivores in the water. Most of the animals, which eat algae, are very tiny and these cells would be difficult for them to ingest. Unicellular Algae Chlorella genus of green algae found either singly or clustered in fresh or salt water and in soil. The alga cell is spherical and has a cup-shaped chloroplast. Chlorella’s reproduction is asexual by nonmotile reproductive cells (autospores). It has been extensively used in photosynthetic studies, in mass cultivation experiments. Because Chlorella multiplies rapidly and is rich in proteins and B-complex vitamins, it has also been studied as a potential food product for humans both on Earth and in outer space. Non-motile unicell • Unicellular Algae Chlamydomonas • Genus of green biflagellated singlecelled organisms of disputed classification, placed botanically in the green algal order, Volvocales, Chlamydomonas is considered a primitive life-form of evolutionary significance. The more or less oval cells have a cellulose membrane (theca), pigment-containing chloroplast. Although photosynthesis occurs, nutrients also may be absorbed through the cell surface. Asexual reproduction is by zoospores; sexual reproduction is by formation of gametes. The development of motility, sexual differentiation,. There are some 500 species of Chlamydomonas Motile unicell COLONIAL ALGAE • These organisms are also found primarily in the aquatic environment. Morphological variation is due to differences in number and plane of cell division only. These algae have only one basic vegetative cell type. Division in definite and consistent planes results in formation of a regular colony while division in random planes results in the formation of irregular colonies. Some colonial algae possess flagella for motility. Again, the major environmental pressure is the need to remain in the photosynthetic zone. Because of the potential increase in sinking rates due to the increase in mass, these algae tend to inhabit shallow waters where the effective light penetration is to the bottom. In addition, many colonial forms have small cells, which secrete mucilage. The mucilage is less dense than water and aids in buoyancy COLONIAL ALGAE Pandorina • Pandorina is a green alga composed of 8, 16, or sometimes 32 cells held together at their bases to form a globular colony surrounded by mucilage. The cells are ovoid or slightly narrowed at one end to appear keystone- or pear-shaped. Each cell has two flagella with two contractile vacuoles at their base, an eyespot, and a large cup-shaped chloroplast with at least one pyrenoid.with at least one pyrenoid COLONIAL ALGAE Pandorina • • The colonies coordinate their flagellar movement to create a rolling, swimming motion. Pandorina shows the beginnings of the colony polarity and differentiation seen in Volvox since the anterior cells have larger eyespots. Molecular sequencing has shown that pandorina is monophyletic Asexual reproduction is by simultaneous division of all cells of the colony to form autocolonies that are liberated by a gelatinization of the colonial envelope. Sexual reproduction occurs by division of each cell of the colony into 16-32 zoogametes. Zoogametes show indications of heterogamy, a slight difference in the size and motility of the pairs that fuse to form the smooth walled zygote COLONIAL ALGAE Volvox • genus of chlorophytes, a type of green algae. It forms spherical colonies of up to 50,000 cells. They live in a variety of freshwater habitats, in 1700. Volvox developed its colonial lifestyle 200 million years ago. COLONIAL ALGAE Volvox • is the most developed in a series of genera that form spherical colonies.Each mature Volvox colon is composed of numerous flagellate cells similar to Chlamydomonas, up to 50,000 in total and embedded in the surface of a hollow sphere or coenobium containing an extracellular matrix made of a gelatinous glycoprotein COLONIAL ALGAE Scenedesmus • is a small, nonmotile colonial green alga consisting of cells aligned in a flat plate. The colonies most often have two or four cells, but may have 8, 16, or rarely 32 and are occasionally unicellular. The cells are usually cylindrical but may be more lunate, ovoid, or fusiform. Typically the end cells each have two long spines up to 200 µm in length protruding from their outer corners, and other cells may have additional spines or chitonous bristles. Each cell contains a single parietal, plate-like chloroplast with a single pyrenoid. The cell walls may be covered in bumps or reticulations that are best viewed with scanning electron microscopy. COLONIAL ALGAE Pediastrum • Pediastrum is a common, nonmotile green alga with flattened colonies of cells in an elaborate starlike pattern. Pediastrum colonies are coenobial - the number and arrangement of cells is genetically determined for each species. The colonies are made up of at least four cells. The cells are often notched or lobed, and sometimes bear short horns or bristles that both improve buoyancy in the water column and help to prevent predation. The cell walls may be smooth or ornamented. The parietal chloroplasts have a single pyrenoid. COLONIAL ALGAE Merismopedia • has cells arranged in perpendicular rows one cell thick to form rectangular colonies. The colonies may be flat or slightly wavy and are held together by colorless, indistinct mucilage. Except for a few species, the colonies are usually microcopic, and may have subcolonies. The cells are usually blue-green or occasionally reddish in color, 1-7 µm in diameter, and are spherical or oval-shaped before dividing and hemispherical after dividing. Some planktonic species have gas vesicles for bouyancy COLONIAL ALGAE Microcystis • A unicellular, planktonic freshwater cyanobacterium. The existence of intracellular structures, the gas vesicles, provides cells with buoyancy. These hollow, gas-filled structures can keep Microcystis cells close to the surface of water body, where there is optimal light and oxygen for growth. the colonies can accumulate at the water surface and form surface water blooms COLONIAL ALGAE Gloeocapsa • may be unicellular or made up of small groups of cells grouped within concentric mucilage envelopes. The individual colonies are usually spherical, microscopic, and enclosed within larger masses of mucilage. The cells are ovalshaped or ellipsoidal, and hemispherical after dividing. Each cell has a rounded, firm, inner mucilaginous sheath surrounded by older sheath material from the parent cell, revealing the pattern of cell division. The sheaths are colorless or vivid shades of yellow, brown, red, orange, blue, or violet, and may be affected by changes in pH. The cells are usually bright blue-green or olive green and do not have distinct gas vesicles