Online supplementary material Appendix 1. Testing the accuracy of age prediction of wooded subcompartments using reflectance bands. The age of each subcompartment was modelled by regressing the recorded number of years since planting against the linear and quadratic terms of the reflectance values of each of the six bandwidths. Each quadratic term was divided by 100 for ease of interpretation. Because of potential non-linear relationships between age and reflectance values, subcompartments with an age greater than 50 were constrained to 50 (Pearce-Higgins et al. 2007). All possible combinations of these twelve variables were modelled, and model averaging across these models used to produce the best model fit to the data (Table A1). Examination of the fit of these models suggested there was still non-linearity between observed and predicted age with an asymptote beyond about 35 years of age. Therefore, to examine the fit of the data, we look only at the strength of the correlation (r 2) between the predictions from these models and the observed ages in the test subcompartments for subcompartments up to 35 years old, which ranged from 36 to 56% accuracy in age prediction, equivalent to that achieved by Pearce-Higgins et al.. (2007). The slope of the relationship between observed and predicted age was then used to correct for any over- or under-prediction in age in each image, to ensure that the three images yielded comparable estimates of forest age. Pearce-Higgins, J.W., Grant, M.C., Robinson, M.C. & Haysom, S.I. 2007. The role of forest maturation in causing the decline of Black Grouse Tetrao tetrix. Ibis 149: 143–155. Table A1. Model averaged coefficients describing forest subcompartment age as a function of Landsat 5 reflectance values for the three satellite images. The accuracy of each model is derived from the correlation between observed and predicted age for the 10% of test data. Image Intercept Band 1 Band 2 Band 3 Band 4 Band 5 Band 7 Band 12 Band 22 Band 32 Band 42 Band 52 Band 72 Accuracy (r2) 206/21 Slope SE -12.9684 -0.0081 0.2702 -0.2288 -0.0281 0.0238 0.3252 -0.0029 -0.5685 0.5002 0.0153 -0.0393 -0.1578 2.7589 0.0121 0.0459 0.0195 0.0070 0.0031 0.0508 0.0115 0.0986 0.0366 0.0060 0.0026 0.0238 0.44 206/20 Slope 204/21 SE 0.1082 0.9980 0.1595 0.0379 0.1076 0.0775 -0.1741 0.0248 0.0101 0.0050 0.0225 0.0029 0.0006 0.0224 -0.1386 0.0337 -0.2148 0.1786 0.3955 0.0493 -0.0260 0.0060 -0.0365 0.0026 -0.0083 0.0080 0.36 Slope SE 8.5192 7.3541 0.0947 0.0739 0.3798 0.1504 -0.4449 0.0661 -0.0253 0.0109 0.0586 0.0088 -0.1120 0.1122 -0.0392 0.0756 -0.9315 0.3310 0.9809 0.1207 0.0083 0.0095 -0.0597 0.0078 0.0377 0.0425 0.56 Appendix 2. Testing the accuracy of predicted cover of non-woodland habitat types. Linear Discriminate Analysis was used to conduct a supervised classification of habitats from colour-composite images produced from bands 2, 3 and 4 in Idrisi Kilimanjaro 14.0 (Clark Labs 2003). After the exclusion of known areas of woodland using existing GIS layers (see main text), polygons delineating ten habitat types (dry heath, wet heath, improved grassland, arable, rough grassland / bog, water, urban, rock, cloud, snow) were distinguished by eye from the remainder of the image. Based on previous relationships with Black Grouse (Pearce-Higgins et al. 2007), only three were of potential interest as explanatory variables (dry heath, improved grassland / arable combined, and rough grassland / bog). Whilst improved grassland / arable habitats can easily be distinguished by this process (Pearce-Higgins et al. 2007), the separation of unenclosed upland habitats might prove difficult. Therefore to test our predictions for dry heath and rough grassland / bog, we correlated predicted cover of these two types against independent habitat cover data derived in the field, from twenty unenclosed upland sites of 2-9.3 km2 in area, collected for the purposes of a separate study (see Pearce-Higgins et al. 2009 for field methods). Observed percentage cover of heather from these 20 sites was used as a measure of dry heath, whilst the observed percentage cover of grasses, sedges and rushes combined was used to provide a measure of rough grassland / bog cover (Pearce-Higgins et al. 2007). Predicted dry heath (x) was strongly correlated with observed heather (y) cover (r = 0.94, n = 20, P < 0.001, y = 5.40 + 0.72x) and predicted rough grassland / bog (x) strongly correlated with observed grass, sedge and rush (y) cover (r = 0.95, n = 20, P < 0.001, y = 27 + 0.63x). The supervised classification therefore provided an accurate map of variation in key vegetation variables across Scotland. Clarke Laboratories. 2003. Idrisi Kilimanjaro 14.0. Clark University, WA, USA. Pearce-Higgins, J.W., Grant, M.C., Robinson, M.C. & Haysom, S.I. 2007. The role of forest maturation in causing the decline of Black Grouse Tetrao tetrix. Ibis 149: 143–155.