PAULITSCHKE A431 Oct 2014
Supporting Information
Table A2. Comparison of keratinocyte-specific proteins (n = 50) versus A431 by number of peptides. *Indicates greater abundance in A431;
**similar abundance in the 2 cell types.
Accession Name
number
Keratinocytes
A431
Difference
(A431keratinocytes)
Function
In the epidermis, expressed predominantly in the granular
layer at apical edge of keratinocytes (at protein level).
Upregulated during keratinocyte differentiation.
Human Protein Atlas: IHC +++
Upregulated in differentiating keratinocytes.
Human Protein Atlas: IHC pending, RNA pos
Cross-linked envelope protein of keratinocytes.
Human Protein Atlas: IHC+
Human Protein Atlas: IHC: pending, RNA: pos +
A. Uniprot
A8K2U0
Alpha-2-macroglobulin-like protein 1
39
0
-39
Q6UWP8
Suprabasin
3
0
-3
P35321
Cornifin-A (19 kDa pancornulin)
3
0
-3
P22528
Cornifin-B
3
0
-3
Q92817
Envoplakin
29
12
-17
P58107
Epiplakin (450 kDa epidermal antigen)
65
0
-65
Q15517
Corneodesmosin (S protein)
3
0
-3
P15924
Desmoplakin
55
0
-55
1
Cornified envelope of keratinocytes; may link the cornified
envelope to desmosomes and intermediate filaments.
Human Protein Atlas: IHC ++
Plakin or cytolinker family.
(Epiplakin 1) Human Protein Atlas: IHC: pending, RNA pos
Important for epidermal barrier integrity. Found in
corneodesmosomes (intercellular structures involved in
desquamation). Exclusively expressed in skin.
Human Protein Atlas: IHC: pos:
Major high molecular weight protein of desmosomes.
PAULITSCHKE A431 Oct 2014
Q02487
Desmocollin-2 (Cadherin family
member 2)
2
0
-2
Q14574
Desmocollin-3 (Cadherin family
member 3; Desmocollin-4; HT-CP)
3
0
-3
Q13751
Laminin subunit beta-3 (Laminin-5
subunit beta; Epiligrin subunit beta;)
Laminin subunit gamma-2 (Laminin-5
subunit gamma)
Laminin subunit alpha-3 (Epiligrin 170
kDa subunit)
10
2
-8
7
1
-6
17
0
-17
O14896
Interferon regulatory factor 6 (IRF-6)
4
2
-2
Q92876
Kallikrein-6
2
0
-2
P49862
Kallikrein-7
8
0
-8
Q13753
Q16787
2
Involved in organization of desmosomal cadherinplakoglobin complexes into discrete plasma membrane
domains and anchoring of intermediate filaments to
desmosomes.
Human Protein Atlas:IHC: +++:
Component of intercellular desmosome junctions: involved
in interaction of plaque proteins and intermediate filaments
mediating cell-cell adhesion. May contribute to epidermal
cell positioning (stratification)
Human Protein Atlas: IHC: +
Component of intercellular desmosome junctions. Involved
in interaction of plaque proteins and intermediate filaments
mediating cell-cell adhesion. May contribute to epidermal
cell positioning (stratification) by mediating differential
adhesiveness between cells that express different isoforms.
Human Protein Atlas: IHC: +++
Binding to cells via a high affinity receptor, laminin is
thought to mediate attachment, migration and organization of
cells into tissues during embryonic development by
interacting with other extracellular matrix components.
Beta 3: Human Protein Atlas: IHC: +
Gamma 2: Human Protein Atlas:IHC: nd, RNA pos
Alpha 3: Human Protein Atlas: IHC:nd, RNA: pos
Probable DNA-binding transcriptional activator. Key
determinant of the keratinocyte proliferation-differentiation
switch involved in appropriate epidermal development
Human Protein Atlas: IHC: pending, RNA: pos
Shows activity against amyloid precursor protein, myelin
basic protein, gelatin, casein and extracellular matrix proteins
such as fibronectin, laminin, vitronectin and collagen.
Human Protein Atlas: IHC: pending, RNA: pos
May catalyze the degradation of intercellular cohesive
structures in the cornified layer in the continuous shedding of
PAULITSCHKE A431 Oct 2014
O43240
Kallikrein-10
11
0
-11
Q9P0G3
Kallikrein-14
2
0
-2
Q6E0U4
Dermokine (Epidermis-specific
secreted protein SK30/SK89)
2
0
-2
Q9Y446
Plakophilin-3
17
3
-14
Q9NZT1
Calmodulin-like protein 5
(Calmodulin-like skin protein)
6
4
-2
P16144
Integrin beta-4**
7
7
0
P07476
Involucrin
23
0
-23
O95171
Sciellin
7
0
-7
3
cells from the skin surface. Abundantly expressed in the skin/
expressed by keratinocytes in the epidermis.
Human Protein Atlas: IHC: ++
Tumour-suppressor role.
Human Protein Atlas: IHC: ++
Serine-type endopeptidase with a dual trypsin-like and
chymotrypsin-like substrate specificity. May function
through desmoglein DSG1 cleavage in epidermal
desquamation, a process by which the most superficial
corneocytes are shed from skin surface.
Human Protein Atlas: IHC: +
May act as a soluble regulator of keratinocyte differentiation.
Human Protein Atlas: IHC: ++
May play a role in junctional plaques. Found in desmosomes
of most simple and stratified epithelia.
Human Protein Atlas: IHC: +++
Binds calcium; may be involved in terminal differentiation of
keratinocytes. Particularly abundant in the epidermis where
its expression is directly related to keratinocyte
differentiation.
Human Protein Atlas: IHC: ++
Integrin alpha-6/beta-4 is a receptor for laminin. Plays a
critical structural role in the hemidesmosome of epithelial
cells; required for regulation of keratinocyte polarity and
motility.
Human Protein Atlas: IHC: ++
Part of insoluble cornified cell envelope (CE) of stratified
squamous epithelia. Keratinocytes of epidermis and other
stratified squamous epithelia.
Human Protein Atlas: IHC: ++
May function in assembly/regulation of proteins in cornified
envelope. Highly expressed in esophagus; also expressed in
PAULITSCHKE A431 Oct 2014
P03973
Antileukoproteinase
3
0
-3
P25815
Protein S100-P
10
2
-8
P29034
Protein S100-A2
5
2
-3
P19957
Elafin (Elastase-specific inhibitor)
3
0
-3
P22735
Protein-glutamine gamma-glutamyltransferase K (Epidermal TGase)
4
0
-4
keratinocytes, amniotic tissue, foreskin stratum spinosum and
stratum granulosum, hair follicle and nail.
Human Protein Atlas: IHC: ++
Acid-stable proteinase inhibitor with strong affinities for
trypsin, chymotrypsin, elastase, and cathepsin G. May
prevent elastase-mediated damage to oral and possibly other
mucosal tissues.
Human Protein Atlas: IHC:nd, RNA: pos
May stimulate cell proliferation in an autocrine manner via
activation of the receptor for activated glycation end products
Human Protein Atlas: IHC:nd, RNA: pos
May play a role in suppressing tumour cell growth; expressed
by a subset of epithelial cells including normal human
mammary epithelial cells and keratinocytes.
Human Protein Atlas: IHC: ++
Neutrophil and pancreatic elastase-specific inhibitor of skin;
may prevent elastase-mediated tissue proteolysis
Human Protein Atlas: IHC: +++
Responsible for cross-linking epidermal proteins during
formation of the stratum corneum.
Human Protein Atlas: IHC: ++
B. Pubmed
P10645
Chromogranin-A (CgA)
2
0
-2
Q5VT79
Annexin A8-like protein 2*
5
8
3
P13928
Annexin A8*
5
9
4
4
Pancreastatin strongly inhibits glucose induced insulin
release from the pancreas. Associated with the secretory
granule membrane to SCG3, that in turn binds to cholesterolenriched lipid rafts in intragranular conditions
Human Protein Atlas: IHC:nd, RNA: pos
A pair of annexin repeats may form one binding site for
calcium and phospholipid.
Human Protein Atlas: IHC: pending, RNA: pos
Anticoagulant protein that acts as an indirect inhibitor of the
thromboplastin-specific complex, which is involved in the
blood coagulation cascade.
PAULITSCHKE A431 Oct 2014
Human Protein Atlas: IHC: pending, RNA: pos
P36952
Serpin B5 (Peptidase inhibitor 5)
21
5
-16
O95633
Follistatin-related protein 3
3
0
-3
O00204
Sulfotransferase family cytosolic 2B
member 1
10
0
-10
O95757
Heat shock 70 kDa protein 4L
9
0
-9
Q9HCY8
Protein S100-A14
7
4
-3
P51178
1-phosphatidylinositol-4,5bisphosphate phosphodiesterase delta-1
7
0
-7
Q14116
Interleukin-18
4
1
-3
Q96QA5
Gasdermin-A
7
0
-7
Q9H1E1
Ribonuclease 7
10
0
-10
5
Tumour suppressor; blocks growth, invasion, and metastatic
properties of mammary tumours
Human Protein Atlas: IHC: +++
Involved in bone formation; inhibits osteoclast
differentiation. Involved in hematopoiesis; involved in
differentiation of hematopoietic progenitor cells, increases
hematopoietic cell adhesion to fibronectin
Human Protein Atlas: IHC: ++
Sulfonation increases the water solubility of most
compounds, and therefore their renal excretion, but can also
result in bioactivation to form active metabolites.
Human Protein Atlas: IHC: +++
Chaperone activity in vitro; inhibits aggregation of citrate
synthase
Human Protein Atlas: IHC: +
Involved in Ca ion binding and cell junction.
Human Protein Atlas: IHC: +++
Production of the second messenger molecules diacylglycerol
(DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by
activated phosphatidylinositol-specific phospholipase C
enzymes.
Human Protein Atlas: IHC: +
Augments natural killer cell activity in spleen cells,
stimulates interferon gamma production in TH-1 cells.
Human Protein Atlas: IHC: +
Found predominantly in the gastrointestinal tract and to a
lesser extent in skin.
Human Protein Atlas: IHC: ++
Skin-derived antimicrobial protein 2. Exhibits a potent
RNase activity; broad-spectrum antimicrobial activity against
PAULITSCHKE A431 Oct 2014
P12532
Creatine kinase U-type, mitochondrial* 10
11
1
P09758
Tumour-associated calcium signal
transducer 2
3
2
-1
many pathogenic microorganisms also in skin.
Human Protein Atlas: IHC: ++
Reversibly catalyzes the transfer of phosphate between ATP
and various phosphogens (e.g. creatine phosphate). Creatine
kinase isoenzymes play a central role in energy transduction
in tissues such as skeletal muscle, heart, brain
Human Protein Atlas: IHC: +++
May function as a growth factor receptor.
Human Protein Atlas: IHC: ++
C. New candidates
Q8TE68
Epidermal growth factor receptor
kinase substrate 8-like protein 1
6
0
-6
Q9UQB8
Brain-specific angiogenesis inhibitor
1-associated protein 2
7
1
-6
O15231
Zinc finger protein 185
6
0
-6
Q9BW04
Specifically androgen-regulated gene
protein
4
0
-4
P11137
Microtubule-associated protein 2
(MAP-2)
4
0
-4
6
Stimulates guanine exchange activity of SOS1. May play a
role in membrane ruffling and remodeling of the actin
cytoskeleton.
Human Protein Atlas: IHC: ++
Adapter protein that links membrane-bound small G-proteins
to cytoplasmic effector proteins. Necessary for CDC42mediated reorganization of the actin cytoskeleton and RAC1mediated membrane ruffling. Involved in regulation of the
actin cytoskeleton by WASF family members and the Arp2/3
complex.
Human Protein Atlas: IHC: ++
May be involved in regulation of cellular proliferation and/or
differentiation.
Human Protein Atlas: IHC: +++
Putative androgen-specific receptor.
Human Protein Atlas: IHC: ++
Exact function of MAP2 unknown but MAPs may stabilize
the microtubules against depolymerisation; also seem to have
a stiffening effect on microtubules.
Human Protein Atlas: IHC:nd, RNA: pos
PAULITSCHKE A431 Oct 2014
Q5K651
Sterile alpha motif domain-containing
protein 9 (SAM domain-containing
protein 9)**
7
8
1
Q4G0N8
Sodium/hydrogen exchanger 10
(Na(+)/H(+) exchanger 10, Spermspecific Na(+)/H(+) exchanger;sNHE)
3
0
-3
7
May play a role in the inflammatory response to tissue injury
and control of extraosseous calcification, acting as a
downstream target of TNF-alpha signaling.
Human Protein Atlas: IHC: ++
Required for the expression and bicarbonate regulation of the
soluble adenylyl cyclase (sAC)
Human Protein Atlas: IHC:nd, RNA: pos
PAULITSCHKE A431 Oct 2014
Table A3. Inflammatory profile in A431: proteins common to keratinocytes stimulated with IL-1 beta (but not present in normal
keratinocytes) and A431 cells (n = 49).
Accession
number
Name
Peptides
Function
Category by GO
terms
O00161
Synaptosomal-associated protein 23 (SNAP23;Vesicle-membrane fusion protein SNAP-23)
1
Cell adhesion
O00625
Pirin
3
O14561
Acyl carrier protein, mitochondrial (ACP;NADHubiquinone oxidoreductase 9.6 kDa subunit;CISDAP)
Deoxyribonucleoside 5'-monophosphate Nglycosidase (c-Myc-responsive protein Rcl)
2
Essential component of the high affinity receptor for the
general membrane fusion machinery and an important
regulator of transport vesicle docking and fusion
Possible transcriptional coregulator. May: contribute to the
regulation of cellular processes via its interaction with BCL3;
be required for efficient terminal myeloid maturation of
hematopoietic cells; play a role in regulation of cell migration;
promote apoptosis when overexpressed.
Carrier of the growing fatty acid chain in fatty acid
biosynthesis in mitochondria.
Acyl-protein thioesterase 2 (Lysophospholipase
II;LPL-I)
Catalase
2
Keratin, type I cytoskeletal 18 (Cytokeratin18;CK-18;Keratin-18;K18;Cell proliferationinducing gene 46 protein)
Cytochrome c1, heme protein, mitochondrial
(Ubiquinol-cytochrome-c reductase complex
cytochrome c1 subunit;Cytochrome c1;Cytochrome b-c1 complex subunit 4)
2
O43598
O95372
P04040
P05783
P08574
8
3
2
2
Transcription,
Oxireductase
Transport,
Lipid
Metabolism
Catalyzes cleavage of the N-glycosidic bond of Nucleotide
deoxyribonucleoside 5'-monophosphates to yield deoxyribose Metabolism
5-phosphate and a purine or pyrimidine base.
May hydrolyze fatty acids from S-acylated cysteine residues in Lipid
proteins such as trimeric G alpha proteins or HRAS.
Metabolism
Occurs in almost all aerobically respiring organisms, protects Oxireductase
cells from toxic effects of hydrogen peroxide. Promotes
growth of cells including T-cells, B-cells, myeloid leukemia,
melanoma and mastocytoma cells and normal and transformed
fibroblasts.
Together with KRT8 is involved in interleukin-6 (IL-6)- Intermediate
mediated barrier protection.
Filament
Heme-containing component of the cytochrome b-c1 complex, Cell junction,
which accepts electrons from Rieske protein and transfers Electron
electrons to cytochrome c in the mitochondrial respiratory transport
chain.
PAULITSCHKE A431 Oct 2014
P11177
Pyruvate dehydrogenase E1 component subunit
beta, mitochondrial (PDHE1-B)
2
P17980
26S protease regulatory subunit 6A (Proteasome
26S subunit ATPase 3;TAT-binding protein
1;TBP-1;Proteasome subunit P50)
Glutathione S-transferase Mu 3 (GSTM3-3;GST
class-mu 3;hGSTM3-3)
2
P24844
Myosin regulatory light polypeptide 9 (Myosin
regulatory light chain 9;Myosin regulatory light
chain MRLC1)
5
P28838
Cytosol aminopeptidase (Leucine aminopeptidase;
Peptidase S)
3-hydroxyisobutyrate dehydrogenase,
mitochondrial (HIBADH)
1
P21266
P31937
2
3
P33316
Deoxyuridine 5'-triphosphate nucleotidohydrolase, 4
mitochondrial (dUTPase;dUTP pyrophosphatase)
P33992
DNA replication licensing factor MCM5 (CDC46
homolog;P1-CDC46)
2
P36639
7,8-dihydro-8-oxoguanine triphosphatase (8-oxodGTPase; Nucleoside diphosphate-linked moiety
X motif 1; Nudix motif 1)
Nuclear pore glycoprotein p62 (Nucleoporin
Nup62; 62 kDa nucleoporin)
1
P37198
9
3
The pyruvate dehydrogenase complex catalyzes overall
conversion of pyruvate to acetyl-CoA and CO2, and thereby
links the glycolytic pathway to the tricarboxylic cycle.
The 26S protease is involved in the ATP-dependent
degradation of ubiquitinated proteins.
Carbohydrate
and
Glucose
metabolism
ATP/nucleotide
binding
Conjugation of reduced glutathione to a wide number of
exogenous and endogenous hydrophobic electrophiles. May
govern uptake and detoxification of both endogenous
compounds and xenobiotics at the testis and brain blood
barriers
Myosin regulatory subunit that plays an important role in
regulation of both smooth muscle and nonmuscle cell
contractile activity via its phosphorylation. Implicated in
cytokinesis, receptor capping, and cell locomotion.
Presumably involved in the processing and regular turnover of
intracellular proteins.
3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3oxopropanoate + NADH
Detoxification
Ca ion binding,
Splicing, Axon
guidance
Proteolysis
Branched-chain
amino
acid
catabolism
Enzyme involved in nucleotide metabolism: produces dUMP, Nucleotide
the immediate precursor of thymidine nucleotides, and metabolism
decreases intracellular dUTP levels so that uracil cannot be
incorporated into DNA.
Acts as component of the MCM2-7 complex (MCM complex), DNA binding,
the putative replicative helicase essential for 'once per cell DNA
cycle' DNA replication initiation and elongation in eukaryotic replication, cell
cells.
cycle
Antimutagenic. Acts as a sanitizing enzyme for oxidized DNA repair
nucleotide pools, thus suppressing cell dysfunction and death
induced by oxidative stress.
Essential component of the nuclear pore complex. The N- Protein
terminal is probably involved in nucleocytoplasmic transport.
transport, NFkappaB
signalling
PAULITSCHKE A431 Oct 2014
P49321
Nuclear autoantigenic sperm protein (NASP)
2
P51970
NADH dehydrogenase [ubiquinone] 1 alpha
subcomplex subunit 8 (NADH-ubiquinone
oxidoreductase 19 kDa subunit; Complex I-19kD;
CI-19kD; Complex I-PGIV; CI-PGIV)
1
P54920
Alpha-soluble NSF attachment protein (SNAPalpha; N-ethylmaleimide-sensitive factor
attachment protein alpha)
Ubiquitin-conjugating enzyme E2 D2 (Ubiquitinprotein ligase D2; Ubiquitin carrier protein D2;
Ubiquitin-conjugating enzyme E2-17 kDa 2;
E2(17)KB 2)
5
Guanine nucleotide-binding protein
G(I)/G(S)/G(T) subunit beta-2 (Transducin beta
chain 2; G protein subunit beta-2)
Nucleobindin-1 (CALNUC)
4
39S ribosomal protein L49, mitochondrial
(L49mt;Protein NOF1; Neighbor of FAU; NOF)
Splicing factor 3B subunit 2 (Pre-mRNA-splicing
factor SF3b 145 kDa subunit; SF3b145; SF3b150;
Spliceosome-associated protein 145; SAP 145)
Phosphomevalonate kinase (PMKase)
1
Hydroxyacyl-coenzyme A dehydrogenase,
mitochondrial (HCDH;)
3
P62837
P62879
Q02818
Q13405
Q13435
Q15126
Q16836
10
2
1
5
1
Required for DNA replication, normal cell cycle progression
and cell proliferation. Forms a cytoplasmic complex with
HSP90 and H1 linker histones and stimulates HSP90 ATPase
activity.
Cellcycle, DNA
replication,
Transport,
Chromatin
remodeling
Accessory subunit of the mitochondrial membrane respiratory Mitochondrial
chain NADH dehydrogenase (Complex I), that is believed not electron
to be involved in catalysis.
transport,
NADH
dehydrogenase
activity
Required for vesicular transport between the endoplasmic Transport
reticulum and Golgi apparatus.
Accepts ubiquitin from the E1 complex and catalyzes its
covalent attachment to other proteins. In vitro catalyzes 'Lys48'-linked polyubiquitination. Mediates the selective
degradation of short-lived and abnormal proteins. Functions in
the E6/E6-AP-induced ubiquitination of p53/TP53.
Guanine nucleotide-binding proteins (G proteins) are involved
as a modulator or transducer in various transmembrane
signaling systems.
Major calcium-binding protein of the Golgi. May have a role
in calcium homeostasis
Structual constituent of ribosome
Ubiquitinconjugationpathway, ATP/
nucleotide
binding
GTPase activity
DNA binding,
Ca ion binding
Translation
Subunit of the splicing factor SF3B required for 'A' complex mRNA splicing
assembly formed by the stable binding of U2 snRNP to the
branchpoint sequence (BPS) in pre-mRNA.
ATP + (R)-5-phosphomevalonate = ADP + (R)-5- Lipid
diphosphomevalonate.
Metabolism,
ATP binding
Plays an essential role in the mitochondrial beta-oxidation of Lipid
short chain fatty acids.
Metabolism,
NAD binding
PAULITSCHKE A431 Oct 2014
Q16891
Mitochondrial inner membrane protein (Mitofilin;
p87/89; Cell proliferation-inducing gene 4
protein)
Epithelial splicing regulatory protein 1 (RNAbinding motif protein 35A; RNA-binding protein
35A)
1
Q7L014
Probable ATP-dependent RNA helicase DDX46
(DEAD box protein 46; PRP5 homolog)
6
Q86VI1
Exocyst complex component 3-like protein
(Protein Jiangli)
39S ribosomal protein L50, mitochondrial
(L50mt)
Sulfatase-modifying factor 2 (C-alphaformylglycine-generating enzyme 2)
1
Q8TEQ6
Gem-associated protein 5 (Gemin5)
1
Q92734
Protein TFG (TRK-fused gene protein)
1
Q92878
DNA repair protein RAD50 (hRAD50)
1
Q96CV9
Optineurin (Optic neuropathy-inducing
protein;NEMO-related protein;Transcription
factor IIIA-interacting protein;TFIIIA-IntP)
1
Q6NXG1
Q8N5N7
Q8NBJ7
11
1
1
3
Interacts with OPA1, preferentially with the soluble OPA1
form.
Mitochondrial
calcium
ion
homeostasis
mRNA splicing factor that regulates the formation of epithelial mRNA
cell-specific isoforms. Specifically regulates the expression of processing and
FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. splicing
Regulates changes in splicing during the epithelial-tomesenchymal transition (EMT)
Plays an essential role in splicing, either prior to, or during mRNA
splicing A complex formation.
processing and
splicing, ATP
binding
As part of the exocyst, may play a role in regulated exocytosis Exocytosis
of insulin granules
Mitochondrion.
Mitochondrion
Lacks formyl-glycine generating activity and is unable to Postconvert newly synthesized inactive sulfatases to their active translational
form.
protein
modification
The SMN complex plays an essential role in spliceosomal mRNA
snRNP assembly in the cytoplasm and is required for pre- processing and
mRNA splicing in the nucleus.
splicing
May be involved in pathogenesis of thyroid papillary NF-kappa
B
carcinoma.
signalling
Component of the MRN complex, which plays a central role in Cell
cycle,
double-strand break (DSB) repair, DNA recombination, DNA
maintenance of telomere integrity and meiosis. The complex damage/repair,
possesses single-strand endonuclease activity and double- ATP binding
strand-specific 3'-5' exonuclease activity, which are provided
by MRE11A. RAD50 may be required to bind DNA ends and
hold them in close proximity.
Plays an important role in the maintenance of the Golgi Cell cycle
complex, in membrane trafficking, in exocytosis, through its
interaction with myosin VI and Rab8.
PAULITSCHKE A431 Oct 2014
Q96DE0
U8 snoRNA-decapping enzyme (Nucleoside
diphosphate-linked moiety X motif 16;Nudix
motif 16;U8 snoRNA-binding protein H29K)
DAZ-associated protein 1 (Deleted in
azoospermia-associated protein 1)
Endoplasmic reticulum resident protein ERp44
(Thioredoxin domain-containing protein 4)
1
Q9BX68
Histidine triad nucleotide-binding protein 2
(HINT-2;HINT-3; PKCI-1-related HIT protein)
2
Q9NRV9
Heme-binding protein 1 (p22HBP)
1
Q9NRX2
39S ribosomal protein L17, mitochondrial
(L17mt;LYST-interacting protein 2)
Methionine adenosyltransferase 2 subunit beta
(Methionine adenosyltransferase II beta;MAT II
beta; DTDP-4-keto-6-deoxy-D-glucose 4reductase)
Glyoxylate reductase/hydroxypyruvate reductase
1
Q9UHY7
Enolase-phosphatase E1 (2,3-diketo-5-methylthio1-phosphopentane phosphatase;MASA homolog)
2
Q9UIJ7
GTP:AMP phosphotransferase mitochondrial
(Adenylate kinase 3;AK 3)
TP53RK-binding protein (PRPK-binding protein)
2
Q96EP5
Q9BS26
Q9NZL9
Q9UBQ7
Q9Y3C4
12
2
1
1
4
1
RNA-binding and decapping enzyme that catalyzes the Nucleotide
cleavage of the cap structure of snoRNAs and mRNAs in a metabolism
metal-dependent manner.
RNA-binding protein
RNA/nucleotid
e binding
Mediates thiol-dependent retention in the early secretory Stress response
pathway. Inhibits the calcium channel activity of ITPR1. May
have a role in the control of oxidative protein folding in the
endoplasmic reticulum.
Hydrolase probably involved in steroid biosynthesis. May play Lipid
a role in apoptosis.
Metabolism,
Apoptosis
May bind free porphyrinogens that may be present in the cell Heme binding
and thus facilitate removal of these potentially toxic
compounds. Binds with a high affinity to one molecule of
heme or porphyrins.
Mitochondrion
Mitochondrion
Non-catalytic regulatory subunit of S-adenosylmethionine
synthetase 2 (MAT2A). Its expression in hepatoma cell lines
may lead to increase DNA synthesis and thereby participate in
cell proliferation.
Enzyme with hydroxy-pyruvate reductase, glyoxylate
reductase and D-glycerate dehydrogenase enzymatic activities
Bifunctional enzyme
One
carbon
metabolism
Oxireductase
Amino acid /
Methionine
biosynthesis
Involved in maintaining the homeostasis of cellular nucleotides Nucleotide
by catalyzing the interconversion of nucleoside phosphates.
binding
Interacts with TP53RK/PRPK.
Protein
catabolic
process.