Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Unit C: BIOLOGY 1.1 A Window on a New World Early Microscopes and Microscopists _____________ and Zacharias Janssen, Dutch lens-makers, invented the ___________________ microscope in 1595. Compound microscopes use more than ________ lens to magnify the object Robert Hooke, in 1665, used a ____________ lense microscope to view cork cells Around the same time Antoni van Leeuwenhoek, using only a single lens microscope was the first to see the movement of single cells that we now know as _________________. Skill and Practice: Calculating Magnification To use a compound microscope to estimate the size of an object you need to be able to calculate the magnification of certain combinations of lenses Magnification = (____________________________________________)(_________________________________) Skill Practice “Calculating Magnification” 1|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 1.1 Check & Reflect 2|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 1.2 Development of the Cell Theory Spontaneous Generation ____________________________ is a theory that states that nonliving things can be transformed into living things. Proposed by Greek philosopher, ___________________ Implied that fish and frogs came from ___________, and flies came from rotten ________ Scientists accepted this theory for nearly _________ years At times referred to as "__________________________ _________________________" Francesco ________, an Italian physician in 1668 set out to prove flies do not come from meat, by conducting an experiment Pieces of meat were placed in ____different jars not covered (______________________________) Pieces of meat were placed in 4 different jars ________________ (______________________) Experimental group -> attracted flies -> laid eggs -> maggot -> fed on meat -> turned to flies Control group ->no fly attraction -> no flies produced Redi concluded flies did not come from rotting ______________ But..._______ was claimed to be the active ingredient to the rotting meat to produce flies So... Redi used an experimental test, this time covering the container with a very fine mesh, allowing for ________ flow, but no fly contact Conclusion... Meat cannot be transformed into ___________. Louis Pasteur (1822-1895) Boiled broth for a long time -> clear broth -> swan-necked flask -> microbes trapped in curve of the flask Conclusion: microbes could not be created from non-living broth 3|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 The Cell Theory No one scientist developed the cell theory. Robert Hooke First described cells in 1665, while looking at a piece of _____________ under a primitive microscope. He saw honeycomb shaped structures and described them as _______________. Anton van Leeuwenhoek Observed living blood cells, bacteria, and single-cell ______________________ Robert Brown 1820, described the tiny sphere in the ________________ cell as the nucleus Theodor Schwann (zoologist) and Mathias Schleiden (botanist) concluded plant and __________________ tissues are composed of cells prepared the __________________ of the modern cell theory The modern Cell Theory states: All living things are made up of ______________ or more cells and the materials produced by these cells. All life functions take place in cells, making them the _______________ unit of life All cells are produced from ________________________________ cells through the process of cell division Homework: Pg 244 Skill Practice “Calculating Magnification” Section 1.1, 246 1.1 Check & Reflect, #1-7 Section 1.2, pg 252 1.2 Check & Reflect, #1-8 (omit 6) 4|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 1.2 Check & Reflect 5|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 1.3 Developments in Imaging Technology and Staining Techniques Contrast Scientist discovered that they could manipulate the __________ source to alter the contrast between structures in the cell and improve the ____________. Experiments with ______________ and coloring agents showed that particular stains could attach to particular parts of the _________, improving the contrast between internal structures and producing better images A disadvantage to staining is that it ___________ the cells, making it impossible to view living cells. Microscopes We are going to be looking at 4 types of microscopes: – ___________________ Microscopes – ___________________ Microscope ________ ___________ – Confocal _______________ Scanning Microscope – _________________ Tunneling Microscope Light Microscope Think of the compound microscope as a good friend It is relatively inexpensive, reliable, but it has its limitations. Maximum magnification ~__________ Electron Microscope The “go to” reliable microscope with lots of power, and just a hint of mystery Engineered in the ___________. Specimens are illuminated with a beam of electrons instead of ___________. Makes electron micrograph. Transmission Microscope Works like a slide projector. Electrons go through a _________ sample to give the micrograph Magnifies _____________________ 6|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Scanning Electron Microscope Sweeps a beam of electrons over object to get a _____ image. Magnification of up to ________________ Confocal Laser Scanning Microscope Has very powerful observation abilities Invented in the ______________ Can study thick specimens, measures ______ slices in order to form a ______ image. Scanning Tunneling Microscope Very technologically advanced. It seems like it can do ANYTHING. Developed in the __________, can obtain an image of atoms on an object’s surface. Can produce images of DNA. Uses an electron probe to make a _______ image Homework: Read Section 1.3 and complete pg 262 1.3 Check & Reflect, #1-6 1.3 Check & Reflect 7|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 2.1 The Cell as and Efficient, Open System Cells carry on all of the life processes including: Intake of ___________________________ ___________________________of gases Movement Waste removal ___________________________ Reproduction Response to stimuli Cell Membrane Protective ___________________________ Allows transport of materials into and out of the cell Composed of ___________________________layer of lipid (fat) molecules and embedded proteins Connects the cell to the ___________________________environment Semi-permeable ___________________________ Regulates the entry of molecules into and out of the cell Nucleus Contains DNA, the genetic material of the cell Directs cellular ___________________________ Surrounded by the nuclear ___________________________, which has pores to allow the transport of materials Cytoplasm __________-like substance inside the cell membrane Contains nutrients required by the cell to carry on the life process ___________________________are suspended in the cytoplasm Allows for the movement of organelles and molecules within the cell, referred to as ___________________________streaming 8|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Cell Wall – plants, bacteria and fungi ___________________________frame around the cell that provides strength and support. Chloroplasts – plants Contain ___________________________that produces a green color, they are the sites of photosynthesis Photosynthesis uses energy from the sun to convert carbon dioxide and water into sugars for the plant’s use and ___________________________ Vacuoles and Vesicles Membrane bound structures that serve to store ___________________________, products of secretion and fats In plants, the central ___________________________stores water for the cell In plant cells, when fluids enter, the central vacuole swells, increasing the turgor pressure causing the cell to become ___________________________. ___________________________transport substances through the cell. Endoplasmic Reticulum Series of interconnected small _________________that branch from the nuclear envelope. Materials can be transported through these tubes Rough endoplasmic reticulum has ___________________________attached to it and is associated with protein synthesis ______________________endoplasmic reticulum is associated with fat and oil production Ribosomes May be attached to the ___________________________reticulum or free in the cytoplasm Sites where ___________________________are assembled into proteins in the process of protein synthesis 9|Page Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Lysosomes Membrane bound sacs in the cell where ___________________________occurs Defend against invading ___________________________ ___________________________damaged cell organelles Control digestion of certain ___________________________during development Golgi Apparatus Flat, disc shaped sacs involved in ___________________________ Receives substances from the endoplasmic ___________________________and packages them for transport out of the cell Mitochondria ___________________________structures Convert chemical energy in sugars into energy the cell can use, cellular respiration 10 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Chemical Composition of Cell Structures Major elements making up the structure of plant and animal cells are carbon, ___________________________, oxygen and ___________________________ They are organized into four major organic compounds: _______________________(fats and oils), ___________________________ (sugars, starches and cellulose), protein (muscle fibre) and nucleic acids (DNA). Similarities: Plant and Animal Both have cell membrane and an internal network of fibres, the ___________________________, made of lipids and proteins Both have genetic material (DNA) made up of sugars, nitrogen bases and _____________ Differences: Plant and Animal Animal cells have ___________________________, involved in cell division Plant cells have cell ___________________________ Plant cells contain ___________________________, used for photosynthesis Plant cells have a large central vacuole, animal cells have small vacuoles and vesicles Homework: Pg. 12, 13 worksheets: due the next day. 11 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Unit C—Cycling of Matter in Living Systems Unit Support Plant and Animal Cells: Give a title to each diagram and complete the labels for each. 12 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Unit C—Cycling of Matter in Living Systems Unit Support Cell Structures and Their Functions Cell Structure Cell membrane Nucleus Cytoplasm Cell Wall Chloroplasts Vacuoles and Vesicles Lysosomes Endoplasmic reticulum Ribosomes Golgi apparatus Mitochondria 13 | P a g e Function Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 2.2 The Role of the Cell Membrane in Transport The cell membrane is the organelle responsible for transport of into and out of the . The substances that enter and leave the cell may be , or in some physical sense are . These substances in a , and behave according to . The Particle Model of Matter 1. All matter is made of but the particles in different substances may be different in . 2. The particles of matter are constantly and most in ; particles move least in . Adding or taking away energy will affect the . 3. The particles of matter are or are . 4. Particles have space between them that are smallest in , except for ice, and greatest in . The spaces may be occupied by the particles of . Diffusion Diffusion is the natural movement of particles from an area of to an area of . Since the particles are in constant motion they move until they are distributed throughout the room, resulting in a state of . Adding energy and increasing molecular movement (i.e. heat and stirring) can increase the . Diffusion can also occur in cells. 14 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 The cell membrane is considered to be because it allows certain particles to pass through it, but . This is called a membrane. Generally the passage of materials through the material is determined by: The of the molecules The of the molecules The of the molecules in lipids (must be soluble) The Concentration Gradient In general, particles move from areas of high concentration to areas of low concentration. Therefore Carbon Dioxide diffuses of the cell, an area of high concentration to an area of low concentration. Oxygen diffuses the cell (low concentration) from outside of the cell where the concentration of oxygen is high. Concentration Gradients Concentration Gradient 15 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Osmosis The cell is an open system, therefore is always interacting and responding to the conditions of the . If the cell membrane is not permeable to the solute mixed with water, the water will pass through the cell membrane, leaving the solute molecules outside of the cell. This is the process of osmosis. Osmosis is the movement of water through a membrane in response to its . The membrane is not to the solute. This is another form of transport. To predict the direction in which a net movement of water will occur, the solute concentration must be compared. Hypertonic ("hyper" means over) - The concentration of the solutes outside is . Therefore if the cell is put into this solution, water will . Isotonic ("iso" means equal) - The concentration of solutes outside the cell is . Therefore if the cell is put into this solution, there is no of water molecules, just odd molecules moving in and out of the cell. Hypotonic ("hypo" means under) - The concentration of solutes outside is . Therefore if the cell is put into this solution, water will the cell. Facilitated Diffusion There are two forms of protein mediated transport for those molecules that are soluble in water but not in lipids, and to cross the cell membrane. a) Channel proteins create pores or channels through which small are able to move. These molecules move in response to the . b) Carrier proteins have the ability to attach to that are not able to across the membrane. The carrier protein and physically moves the molecules across the 16 | P a g e and Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 into the . Once the molecules have been transported, the its returns to . This process is called a , which is the movement of substances in response to the concentration gradient but also requires the presence of the protein facilitator. Both forms of protein mediated transport are examples of passive transport, because no added needed. Active transport requires energy input to transport molecules. Active transport is necessary when protein carriers need to carry molecules across the , from areas of to areas of . The energy required is produced in the through the process of and comes from the substances called . A series of chemical reactions occurs first in the cytoplasm then in the mitochondrion, to break down and produce the ATP. Endocytosis and Exocytosis When a molecule need to be taken in by the cell, but are too large to pass across the cell membrane, event with the help of protein carriers, the process of Endocytosis occurs. Endocytosis is the process that occurs when a (a small temporary sac) forms around the particle and the cell membrane pinches off around it so that the vesicle is inside the cell. When a molecule need secreted by the cell, but are too large to pass across the cell membrane, a vesicle surrounds the particle, then moves to the vesicle then and fuses with it. The , releasing its contents into the surrounding area of the cell. This process of secretion is called exocytosis. Both exocytosis and endocytosis require energy from ATP for the rearrangement of the cell membrane. Homework: Worksheet 17 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.1 Cells, Tissues, and Systems and 3.2 The Leaf and Photosynthesis Plant Structure Plants are ___________________________organisms Living systems made up of many parts Groups of cells performing the same function together are called tissues ___________________________contributing to the same function form organs which are part of a system The plant has two organ systems: the ___________________________system (everything above ground) and the ___________________________system (everything below ground) Plants grow, or increase in size by cell division in the Shoot apical ___________________________, or ___________________________apical meristem There are 3 main types of plant tissues: Dermal tissue: ___________________________ Ground tissue: ___________________________material Vascular tissue: ___________________________and ___________________________ Dermal Tissue Dermal tissue or epidermis is the outer layer of cells that covers all ___________________________ (non-woody) plants. Responsible for the exchange of matter and gases into and out of the plant In woody plants, the epidermis is replaced by cork and bark, the leaves and stem are responsible for gas exchange of carbon dioxide and oxygen Protects the plant from ___________________________ Dermal tissue of the root system is responsible for the uptake of water and ___________________________salts from the soil. 18 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Ground Tissue Makes up the ___________________________of the plant, found beneath the epidermis In the stem they provide ___________________________and support to the plant In the roots they are involved in food and water storage In the leaves it is the location where ___________________________occurs Cells are loosely packed and allow gases to diffuse rapidly through the ground tissue. Vascular Tissues Vascular tissue is responsible for moving materials around the plant. Water from the roots to the leaves, and ____________________from the leaves to the roots for storage Xylem Tissue Moves water and dissolved ___________________________from the roots up the stem to the leaves where these substances are used in photosynthesis. Thick walled tubes of various diameters Phloem Tissue Transports ___________________________and other dissolved sugars from the leaves to other parts of the plant Formed from individual long sieve tube cells, which have perforated end walls Specialization in Plant Cells Cells that become part of the root system and are responsible for the ___________________________of water and minerals from the soil, produce tiny hair-like projections called root hairs Root hairs increase the surface area for absorption Dermal cells of the shoot system produce ___________________________to protect the cells from water loss ___________________________epidermal surface of leaves develops specific cells, called guard cells Guard cells form tiny pores called ___________________________for gas exchange Guard cells contain ___________________________ 19 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Photosynthesis Leaves perform ___________________________in plants, and this process supports all life on earth. Chloroplasts are found in the leaves of plants and contain all the ___________________________, which is responsible for photosynthesis. ___________________________is a chemical process that transforms CO2 and H2O into useable materials such as C6H12O6 and O2. Balanced Chemical Equation : Plants can now use this simple sugar to undergo normal life processes. (Energy). Cellular Respiration This is the description of the normal activities inside the plant. (Growing, breathing, waste excretion, etc) These processes require energy, and that energy comes from the sugars (glucose) produced during photosynthesis. The process of cellular respiration occurs in the mitochondrion The plant transforms some of the glucose produced as follows: Homework: 3.1 Check and Reflect Page 302 #1-3, 5 and 3.2 Check and Reflect Page 308 #1-5, due the next day. 20 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.1 Check and Reflect 21 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.2 Check and Reflect 22 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.3 The Leaf and Gas Exchange Dermal Tissue The ___________________________on the top and underside of the leaf is clear and very thin Guard cells form tiny openings or pores called ___________________________allow gas exchange to occur Stomata regulates the movement of gases and open into air chambers that connect with the cells of the ground tissue Carbon dioxide and oxygen can enter and leave the leaf by ___________________________at any time. Most ___________________________are located on the underside of the leaf ___________________________cells function to allow materials in and out when necessary Protect the leaves from losing too much water The process of water leaving the leaf through the stomata is called transpiration Without guard cells transpiration could dangerously dehydrate a plant The number of stomata vary depending on environmental conditions: hot, dry climate = _____________________stomata, high humidity = _______________________stomata Ground Tissue Between the upper and lower epidermis are specialized ground tissues called mesophyll There are two different types of ___________________________tissues 23 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Palisade Tissue Cell Found just below the upper ___________________________ Long, rigid, rectangular cells that are tightly packed together and arranged so that a large number of cells are exposed to the Sun’s rays. Responsible for ___________________________ Require ___________________________and produce ___________________________ Spongy Mesophyll Tissue Located between the ___________________________tissue cells and the lower epidermis Loosely packed irregularly shaped cells Increased space allows for gas exchange by ________________________throughout the leaf Move oxygen towards the stomata or expulsion from the plant and will move carbon dioxide towards the ___________________________cells Vascular Tissue Provides the leaf with the water needed for ___________________________and photosynthesis and removes the sugars formed The “ribs” running through the leaf, called veins, contain the vascular tissue of the leaf. The xylem and phloem tissues are bunched together like a handful of straws in a vascular bundle Xylem transports water necessary for ___________________________and dissolved salts from the roots to the leaf Phloem transports the sugar manufactured in ___________________________to the rest of the plant The vascular bundles are direct extensions of the vascular bundles of the stem They branch into finer veins within the spongy ___________________________ Gas Exchange in Plants All gas exchange occurs by diffusion Diffusion occurs through air spaces and then across cell membranes Homework: 3.3 Check and Reflect p. 314 # 1 – 6 24 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.3 Check and Reflect 25 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.4 Transport in Plants and 3.5 Control Systems Cohesion and Adhesion The attraction of one water molecule to another is ___________________________ Cohesion is due to the ___________________________nature of the water molecule Adhesion is the ability of water molecules to ___________________________other molecules Adhesion is also due the polarity of water molecules Tension or Transpiration Pull The ___________________________of water through the stomata during the process of transpiration creased a tension or transpiration pull As each molecule ______________________, it creates a pull on the adjacent water molecules Combine this with the forces of adhesion and cohesion it draws water up the xylem to the leaves The Effect of Tonicity of Plant Cells ___________________________is the concentration of solute particles in a solution In a ___________________________environment, the plant appears wilted In a ___________________________environment, water enters by osmosis and the cells become turgid Phototropism “photo” means ___________________________, and “tropism” means ___________________________ Stems exhibit ___________________________phototropism, they grow towards the light Roots exhibit negative phototropism, they grow ___________________________from the light Gravitropism Stems grow against the gravitational force and so show ______________________gravitropism while roots grow towards the gravitational force and so show _______________gravitropism Homework Tropism Concept Map 3.4 Check and Reflect p. 322 #1-5 and 3.5 Check and Reflect p. 328 # 1-3 26 | P a g e Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 Discovering Tropisms: Concept Map What to Do Complete the concept map below, using the following words and phrases. gravitropism day roots phototropism touch night elongate do not elongate 3.4 Check and Reflect 27 | P a g e turgor pressure at base of leaves stems day and night Science 10 [CYCLING OF MATTER IN LIVING SYSTEMS] Semester 1 – 2015/2016 3.4 Check and Reflect 3.5 Check and Reflect 28 | P a g e