Chapter 4 Cells and Energy I. Chemical Energy and ATP A. Chemical energy used for most cell processes is carried by ATP 1. Carbohydrates and lipids are the most important energy sources in foods we eat 2. Adenosine triphosphate (ATP) carries the chemical energy used by cells 3. ATP has three phosphate groups, the bond holding the third phosphate group is unstable and easily broken. Energy is released when this bond is broken and adenosine diphosphate is formed ATP ADP + P + energy B. Organisms break down carbon-based molecules to produce ATP 1. The number of ATP molecules produced depends on the type of molecule broken down a. lipids yield the most ATP (triglycerides 146 ATPs) b. carbohydrates yield the next most ATP (glucose 36 ATP) c. proteins yield less ATP C. Some organisms do not need sunlight and photosynthesis as a source of energy 1. plants get energy directly from sunlight (photosynthesis) 2. animals get their energy indirectly from sunlight from the foods they eat 3. some organisms get their energy from chemicals such as hydrogen sulfide (H2S) in the process of chemosynthesis II. Overview of Photosynthesis A. Photosynthetic Organisms are Producers 1. Producers provide energy for themselves and other organisms 2. Photosynthesis – a process that captures energy from sunlight to make sugars that store chemical energy 3. Chlorophyll – molecule in chloroplasts that absorbs some of the energy in visible light B. Photosynthesis in plants occurs in chloroplast 1. Two main parts of chloroplast a. grana – stacks of coined-shaped, membraneenclosed compartments called thylakoids b. stroma – fluid that surrounds the grana inside a chloroplast 2. Light-dependent reaction – captures energy from sunlight a. chlorophyll absorbs energy from sunlight. The energy is transferred along the thylakoid membrane. H2O molecules are broken down. Oxygen molecules (O2) are released b. Energy carried along the thylakoid membrane is transferred to molecules that carry energy, such as ATP 3. Light-independent reaction – uses energy from the light-dependent reaction to make sugars a. CO2 is added to a cycle of chemical reactions to build larger molecules, using energy from the lightdependent reaction b. A molecule of a simple sugar is formed, usually glucose (C6H12O6) 4. Overall reaction for photosynthesis 6CO2 + 6H2O C6H12O6 + 6O2 carbon dioxide water light, enzymes glucose oxygen III. Photosynthesis in Detail A. Light-Dependent Reaction 1. Photosystem II and Electron Transport Chain a. Energy absorbed from sunlight – light-absorbing molecules in the thylakoids absorb sunlight, energy is transferred to electrons (e-), the high energy electrons enter the electron transport chain b. Water molecules split – enzymes break down water molecules, hydrogen ions (H+) and electrons are separated from each other, oxygen (O2) is released, electrons from water replace electrons that entered the electron transport chain c. Hydrogen ions transported – Electrons move from protein to protein in the electron transport chain, their energy is used to pump H+ ions into the thylakoid against the concentration gradient, electrons move on to Photosystem I 2. Photosystem I a. energy is absorbed from sunlight into the lightabsorbing molecules, electron are energized and leave the molecules b. NADPH is produced – energized electrons are added to a molecule called NADP+ and a molecule of NADPH is formed and moves on to the lightindependent reaction c. Hydrogen ion diffusion – hydrogen ions flow through a protein channel out of the thylakoid membrane d. ATP produced – the protein channel is part of a complex enzyme called ATP synthase, as the ions flow through the channel, ATP synthase makes ATP B. Calvin Cycle (light-independent reaction) 1. The Calvin Cycle takes place in the stroma a. carbon dioxide is added – CO2 molecules are added to five-carbon molecules already in the Calvin cycle b. Three-carbon molecules are formed – ATP and NADPH from the light-dependent reaction is used by enzymes to split the six-carbon, three-carbon molecules are formed and rearranged c. Three-carbon molecules exit – most of the threecarbon molecules stay in the Calvin Cycle, after two three-carbon molecules have left the cycle, they are bonded together to build a six-carbon sugar such as glucose d. Three-carbon molecules are recycled – energy from ATP is used to change the three-carbon molecules back to five-carbon molecules to be used in the Calvin Cycle C. Functions of Photosynthesis 1. Captures the energy of light and makes chemical energy (food) 2. Releases Oxygen to be used by consumers IV. Overview of Cellular Respiration A. Cellular Respiration makes ATP by breaking down sugars 1. Cellular Respiration is an aerobic process 2. Glycolysis – splits a glucose molecule into two threecarbon molecules and makes two molecules ATP 3. Glycolysis is an anaerobic process 4. Kreb Cycle – produces molecules that carry energy to the second part of cellular respiration B. Overall Reaction for cellular respiration C6H12O6 + 6O2 6CO2 + 6H2O + energy Glucose Oxygen Carbon Dioxide water V. Cellular Respiration in Detail A. Glycolysis is needed for cellular respiration 1. Two ATP molecules energize a glucose molecule and split it into two three-carbon molecules 2. NADPH is formed from the electrons from the threecarbon molecules, pyruvate is made from the threecarbon molecules, 4 ATP molecules are made 3. A net of two ATP molecules are made in glycolysis B. Krebs Cycle 1. Pyruvate broken down into a two-carbon molecule and carbon dioxide, NADH is formed and moves to the electron transport chain 2. Coenzyme A bonds to two carbon molecule and goes to Kreb cycle 3. Citric Acid formed – two-carbon molecule is added to a four-carbon molecule to form a six-carbon molecule 4. Citric Acid is broken down – an enzyme breaks the six-carbon molecule down to a five-carbon molecule 5. Five-carbon molecule is broken down, a four-carbon molecule, a molecule of NADH, and a molecule of ATP are formed 6. Four-carbon molecule is rearranged C. The electron transport chain 1. Electrons are removed from NADH and FADH2 2. Hydrogen ions transported along electron transport chain 3. ATP produced, for each pair of electrons that pass through the electron transport chain, 3 ATP molecules are made 4. Water is formed D. Cellular respiration can make 38 ATP molecules from 1 glucose molecule, but it used 2 ATP molecules for glycolysis VI. Fermentation A. Fermentation allows glycolysis to continue in the absence of oxygen 1. Pyruvate and NADH from glycolysis enter the fermentation process 2. Two molecules of NAD+ are recycled back to glycolysis B. Products of fermentation 1. Lactic Acid – builds up in muscles during periods of anaerobic exertion 2. Alcohol 3. Bread 4. Cheese, yogurt 5. Human digestion