AP Biology 2 Notes Chapter 10 Photosynthesis Introductory Vocab • • • • • • • Heterotrophs Autotrophs Chemoautotrophs Mesophyll Stroma Grana Thylakoids PHOTOSYNTHESIS • The equation: – CO 2 + H 2 O C H 2 O + O 2 • Light reactions (photo) occur in thylakoids – Changes light energy to chemical energy making NADP and ATP • Calvin cycle (synthesis) occurs in the stroma – Uses the NADP and ATP to make sugar Label the Parts Light Review • • • • Wavelength ROYGBIV Photons Beneficial for photosynthesis • Reflection and absorption in plants • Spectrophotometer Chlorophyll • • • • Chlorophyll a accessory pigments Chlorophyll b Carotenoids Chlorophyll Molecule Photosystems • Chlorophyll is organized into photosystems • These include an antenna complex, reaction center chlorophyll and a primary electron acceptor • Photosystem I - contains chlorophyll a and makes NADPH • Photosystem II makes ATP Converting light energy to chemical energy Light Energy Light Energy Electron Flow • Non cyclic electron flow – occurs in thylakoids and uses both photosystems – Passes electrons from water to NADPH – Produces ATP and NADPH and oxygen by splitting water • Cyclic electron flow – makes only ATP and uses Photosystem II – This ATP will be used in the Calvin cycle Chemiosmosis Differences • MITOCHONDRIA • Electrons come from food • Membranes pump protons • CHLOROPLAST • Uses light energy • Thylakoid membrane pumps protons from stroma to thylakoid compartment and back Light Reactions and Chemiosmosis Chemiosmosis Similarities • Both mechanisms: – work with ATP synthetase to produce ATP from ADP – have similar electron carriers – ETC moves protons across a membrane down an electromagnetic gradient Calvin Cycle • Similar to Krebs because the starting material is regenerated • Carbon enters as CO 2 and leaves as sugar • Produces G3P • Uses ATP 3 Phases of Calvin Cycle • Carbon Fixation – CO2 attaches to ribulose biophosphate (RuBP) which is the starting material – Rubisco is the enzyme that catalyzes this step • Reduction – Transfers a P from ATP to another molecule – Removes electrons from NADPH • Regeneration of RuBP – Uses 3 ATP molecules to make 1 RuBp Calvin Cycle Summary • To produce one 6 carbon sugar (glucose) it takes 18 ATP and 12 NADPH! • The cycle must turn 6 times to make 1 glucose Photorespiration • Occurs on very hot dry days • Occurs in C3 plants like rice, wheat and soybeans • Rubisco can accept O2 to make a 2 carbon molecule which the mitochondria and perioxisomes can change to CO2 • This produces not ATP or food • Uses Calvin cycle products • Seems useless but probably left over from day when there was more CO2 in air • Reduces production of crops C4 plants • These include sugar cane and corn • Works in very hot, dry regions • This keeps the plant from going through photorespiration • PEP carboxylase attaches to CO2 more easily than rubisco • When CO2 levels are low, this can change CO2 even when rubisco cannot CAM plants • Cacti, pineapple • Occurs in very dry regions • These open the stomata only at night to go through photosynthesis…but no light!! • Bring in CO2 during the night and change to an organic acids that is stored in the vacuoles • During light, organic acids are released and changed back to CO2