Chapter 1 - Introduction: Exploring Life NEW AIM: What are the major characteristics of life? Figure 1.1 Chapter 1 - Introduction: Exploring Life AIM: What are the major characteristics of life? Chapter 1 - Introduction: Exploring The Scientific Life Study of Life lifethe organized? AIM: How Whatisare major characteristics of life? Biology? The study of life (organisms and how they interact with the biotic and abiotic components of their environments or surroundings) How does one define life or living? Chapter 1 - Introduction: The Scientific Exploring Life Study of Life AIM: How all life Whatis are theunited? major characteristics of life? Characteristics of Life (Living vs. Non-Living) Chapter 1 - Introduction: Exploring Life 1. Organization/order All life is ordered/organized, which is why energy is always needed (must maintain this order). The atoms that make us up can be arranged in a countless number of ways, but only a handful of those will result in you…a working organism. Any arrangement outside of this handful results in death. Recall the 2nd law of the universe With every transfer of energy, thermodynamics… becomes more disordered. There are MANY energy transfers within and around you resulting in disorder, and therefore you must constantly be maintaining order. Fig. 1.3 Chapter 1 - Introduction: Exploring Life 1. Organization Hierarchy of Life Going from the basic level of subatomic particles (protons, electrons, neutrons, etc…) to the more complex level of ecosystems Emergent Properties Notice as we move up the hierarchy, new properties come to be that were not there before. For example, atoms alone like carbon, nitrogen, oxygen, hydrogen and phosphorus cannot store information to build organisms, but combine them to form a molecule called DNA and you now have a new property…information Fig. 1.3 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: 1. Atoms (carbon, nitrogen, oxygen, hydrogen, etc…) combine to form molecules (proteins, carbohydrates, lipids, nucleic acids, vitamins). 2. Molecules combine to form organelles (nucleus, ribosomes, plasma membrane, ER, golgi, etc…) Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: 3. Organelles combine to form cells What is a cell? The most basic unit of life (the lowest level on the hierarchy that can perform all the functions of life) Let’s take a closer look at cells… Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How can is are allwelife united? AIM: What the major characteristics of life? AIM: How observe cells? Cell Theory New cells come from pre-existing cells with the exception of the first cell, and that the cell is the most basic unit of all living organisms. Credit for this theory is given to: Theodor (German Physiologist) Schwann Matthias Jakob Schleiden (German Botanist) Cell is basic unit of life Rudolf Virchow (German Doctor, Biologist,… All cells from pre-existing ce Chapter 1 3 - Introduction: The MoleculesExploring The of Cells Scientific Life Study of Life How can is are allwelife united? AIM: What the major characteristics of life? AIM: How observe cells? Scaled Size of a cell Imagine Madison Square Garden (MSG), to be a cell…how big is a protein in this cell? Reminder, a protein in the cell would be like a tennis ball in MSG and the DNA would be like a rope with a thickness of 2mm and a length of 1200 miles. Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How is are all life AIM: What theunited? major characteristics of life? Two major forms of cells evolved on Earth 1. Prokaryotes (bacteria or the kingdom monera) - Two types A. Eubacteria DNA in blue, ribosomes are the black d B. Archaebacteria - No internal membranous organelles No nucleus, ER, golgi, lysosomes, peroxisomes, etc… - First to evolve, simpler Pro-, coming before. Karyo = nucleus Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How is are all life AIM: What theunited? major characteristics of life? Two major forms of cells evolved on Earth 2. Eukaryotes (animals, plants, protists, fungi) - A fusion of prokaryotes Endosymbiotic theory Mitochondria and Chloroplasts were once free-living prokaryotes that at some point in evolution cooperated with another larger prokaryote to eventually become one…the eukaryotic cells. Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How is are all life AIM: What theunited? major characteristics of life? Two major forms of cells evolved on Earth 1. Eukaryotes (animals, plants, protists, fungi) - A fusion of prokaryotes - Membranous organelles Nucleus, ER, golgi, lysosomes, peroxisomes, etc… - More complex Eu-, true. Karyo = nucleus DNA found inside the purple nucle Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How is are all life AIM: What theunited? major characteristics of life? Two major forms of cells evolved on Earth EUKARYOTIC CELL PROKARYOTIC CELL DNA Membrane (no nucleus) Membrane Cytoplasm Organelles Figure 1.8 Nucleus (contains DNA) 1 µm Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How all life AIM: are theunited? major characteristics of life? NEW What AIM:isHow are eukaryotic cells organized? Does more complex mean better? More complex means more that can break, more to maintain against the 2nd law of thermodynamics, more to form(longer development time), etc… Both types of cell exist on Earth and therefore they both work. If it works it works…neither is “better”. If eukaryotes go extinct, then you might say the prokaryotes were better, but only better in the environment that caused Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How all life AIM: are theunited? major characteristics of life? NEW What AIM:isHow are eukaryotic cells organized? Does more complex mean better? Chapter 1 The Life Study of Life 4 - Introduction: A Tour of theExploring CellScientific How is are all life AIM: What theunited? major characteristics of life? The Basketball, The Size Comparison Analogy Battery and the Pin Head Eukaryotic cell (1/100th to 1/10th mm) Prokaryotic cell 1/1000th to 1/100th mm Virus (just the pin head) 1/20,000th mm Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: Figure 1.5 Figure 1.5 – a eukaryotic cell undergoing mitosis. Chromosomes (DNA plus associated proteins) are stained blue, microtubules are green and the cytoskeleton is red. The left panel is anaphase and the right is telophase. Let’s briefly focus on the chromosomes / DNA…structure and function? Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: Structure of DNA: Figure 1.7 DNA nucleotide monomers (A,C,T,G) combine to form the double-stranded DNA polymer. DNA (deoxyribonucleic acid) – two stranded polymer of nucleotides A, T, C and G forming a double helix. Base pairs between strands: A pairs with T C pairs with G Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: Figure 1.7 Structure of DNA: DNA nucleotide monomers (A,C,T,G) combine to form the double-stranded DNA polymer. Structure determines function… The structure of a hammer determines its function to hit nails. Likewise, the structure of a pencil allows for its function to write. How does the structure of DNA relate to its function? Chapter 1 3 - Introduction: The MoleculesExploring The of Cells Scientific Life Study of Life Howdoisorganisms all life AIM: build/break macromolecules? AIM:How What are theunited? major characteristics of life? 1. Organization The hierarchy of life: Function of DNA / chromosomes: DNA (a chromosome) is a book written in only 4 letters!! The DNA, like all books, stores information. What is this information? Instructions to build every RNA and polypeptide (protein) in the cell. DNA is found in the nucleus of eukaryotic cells or the nucleoid region of prokaryotic cells. Each piece called a chromosome. They are books making the nucleus of eukaryotic cells the library. All of the chromosomes in a cell is called the genome. Chapter 1 3 - Introduction: The MoleculesExploring The of Cells Scientific Life Study of Life Howdoisorganisms all life AIM: build/break macromolecules? AIM:How What are theunited? major characteristics of life? 1. Organization The hierarchy of life: Function of DNA / chromosomes: DNA is the hereditary molecule of life Sperm cell Nuclei containing DNA Figure 1.6 Egg cell Fertilized egg with DNA from both parents Embyro’s cells with copies of inherited DNA Proteins [and in certain cases RNA] built you, but the information to build them is stored in the DNA. The books are passed on to the next generation (heritable)… Offspring with traits inherited from both parents Chapter 1 3 - Introduction: The MoleculesExploring The of Cells Scientific Life Study of Life Howdoisorganisms all life AIM: build/break macromolecules? AIM:How What are theunited? major characteristics of life? 1. Organization The hierarchy of life: Function of DNA / chromosomes: Genetic Variation We all obviously have slightly different books (chromosomes) that code for slightly different proteins/RNA that will in turn build us slightly differently. Why is genetic variation important? Because the environment is changing around us. If we were all identical, a change that caused one of us to die would cause all of us to die…. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: 3. Organelles combine to form cells (muscle cells, neurons, blood cells, etc…) 4. In multicellular organisms, cells combine to form tissues: muscle tissue, nervous tissue, connective tissue, and epithelial tissue (there are only 4 types). 5. Tissues combine to form organs like the heart, lungs, pancreas, liver etc… Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: The regents focuses mainly on the immune, reproductive and endocrine systems 6. Organs combine to form the organ systems: Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: Function is to obtain nutrients – proteins, carbohydrates, lipids, nucleic acids, vitamins, minerals. Nutrients are first ingested, then digested (large molecules broken down into smaller ones – protein to amino acids, polysaccharides to monosaccharides, nucleic acids to nucleotides, etc… so they can be absorbed by the small intestines into the blood for transport). All undigestable material (roughage/fiber/cellulose) will be egested. Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: Serves for gas exchange. Bring in molecular oxygen (O2) to your blood, which will be used to perform cell respiration by your cells. Get rid of CO2 and H2O waste (excrete) in the blood coming from cell respiration. Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: The heart pumps blood around your body in hollow tubes made of cell called arteries (take blood away from heart), veins (bring blood toward heart), and capillaries (where nutrients and waste diffuse to cells). These would be the roadways of your body. The blood also carries other substance like white blood cells (WBC’s – immune system) and red blood cells (carry oxygen). Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: Cleans the blood of metabolic waste (waste from chemical reactions) like H2O from cell respiration, and urea (form of nitrogenous waste) from deamination. The kidney are “blood filters”, removing urea, excess salt or excess water and other undesirable chemicals from the blood. Although not shown, the respiratory system has excretory function in the elimination of CO2 since it is a gas along with the skin, which will release some urea, salt and water as sweat. Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: CELLS TALK TO EACH OTHER (cell signaling)… Your cells must be able to talk to each other in order to cooperate to maintain homeostasis, and to be able to survive and reproduce… ***Homeostasis is NOT EQUILIBRIUM!! In order to be alive, we cannot be at equilibrium. Homeostasis maintains a specific internal environment (order), which is far from equilibrium. Think about your body temperature…is this at equilibrium? We are endotherms (warm-blooded) and maintain a body temp not at equilibrium with our surroundings typically. Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: Your cells must be able to talk to each other. For example, insulin from the pancreas instructs the liver to take up glucose from the blood. The endocrine system uses hormones (special chemicals – usually amino acid based - ex. Insulin, or steroid (lipid) based – ex. testosterone) put into the blood by endocrine cells. The hormone will bind to receptor proteins on target cells and signal them to perform certain functions. This system is typically slow and long term – takes a while for the hormones to circulate and they will stay around a while… CELLS TALK TO EACH OTHER (cell signaling)… Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: The nervous system is also all about cellular communication, but instead of using chemicals released into the blood, it uses cells that act like wires called neurons that transmit electrical signals. These signals are extremely fast (up to 120 m/s) and are typically short lived. An example would be moving your finger or any other skeletal muscle. The electrical signal is sent from your brain, down your spinal cord and out to the muscle in your finger. CELLS TALK TO EACH OTHER (cell signaling)… Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: There are millions upon millions of bacteria, fungi, parasitic worms, etc… that would love nothing more than to take your organic molecules and make them lunch. Luckily you have an immune system. This systems protects your body from foreign substance. White blood cells are responsible for the destruction of these substances. They, like all blood cells, are born in the bone marrow and secrete antibodies (a type of protein) that will stick to foreign molecules called antigens on bacteria, viruses, etc... Anything antibodies stick to will be eaten by other white blood cells. Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: What is life without a reproductive system? Nonexistent. This one if fairly self-explanatory. It is also the only system not required for the organism itself to survive. Gametes (sex cells = sperm and egg/ovum) are made in the gonads (testes and ovaries). Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: The muscular system is composed of all the skeletal muscles of the body. The skeletal muscles allow the body to move when combined with signals from the nervous system (and a lot of ATP of course). Ligaments attach bone to bone. Tendons attach muscle to bone. Muscle cells can ONLY contract (get shorter) – you cannot signal a muscle to extend. Life is simply a series of movements (energy transfers). Fig. 20.9 Chapter - Introduction: The Exploring Scientific Life Study of Life Chapter120: Unifying Concepts of Animal Structure and Function How all life AIM: are theunited? major characteristics of life? AIM: What Howisare animals organized? Organs cooperate to build organ systems: The skeletal system serves to support the body (without it you would be a blob on the floor), protects vital organs like your lungs, brain, spinal cord and heart, and contains marrow that produces blood cells. The integumentary system is composed of the skin, hair and nails (feathers and scales), all of which are mostly non-living. This system serves to waterproof, cushion and protect the deeper tissues, excrete wastes, regulate temperature, is the attachment site for sensory receptors to detect pain, sensation, pressure and temperature, and may attract a mate. Integere means “to cover” (latin) Fig. 20.9 Chapter Introduction: The Life Study of Life Chapter 51--Energy and the Exploring Cell Scientific How is are all AIM: Describe thelife process of active transport. AIM: What theunited? major characteristics of life? Comparing single to multicellular organisms Function Gas Exchange Transport Nutrition Excretion Single Cell Multicellular Chapter Introduction: The Life Study of Life Chapter 51--Energy and the Exploring Cell Scientific How is are all AIM: Describe thelife process of active transport. AIM: What theunited? major characteristics of life? Comparing single to multicellular organisms Function Single Cell Multicellular Gas Exchange Cell Membrane Respiratory System Transport Cytoplasm (cyclosis) Circulatory System Nutrition Lysosomes Digestive System Excretion Cell membrane Excretory System and others Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization Organ systems cooperate: Ex. Nutrients from digestive system are transported to cells by circulatory system Ex. Hormones from the endocrine system regulate the reproductive system Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization Organ systems cooperate: Ex. When body temp drops, nerve impulses from brain signal muscles to contract and shiver Ex. Waste products from chemical reactions are transported by the circulatory system to the kidney/lungs/skin for removal (excretion). Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization The hierarchy of life: 7. Organ systems combine to form organisms 8. Organisms that can reproduce with each other collectively form a population (species) 9. Different populations combine to form communities (squirrels, birds, humans all living in an area). 10. Communities (biotic-living) combine with the rest of the abiotic (non-living) substances like soil, water, air to form the ecosystem. Let’s take a closer look at ecosystems… Chapter 1 - Introduction: Exploring The Scientific Life Study of Life NEW What How AIM: is How allare life organisms united? to the environment? AIM: are the majorconnected characteristics of life? Producers = autotrophs = organisms that make their own food (food = organic molecules) by photosynthesis or chemosynthesis Consumer = heterotroph = steal/obtain food (organic molecules) from the producers or other consumers Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How organisms connected to the environment? How are is are all life AIM: What theunited? major characteristics of life? What does this diagram show/tell us? Chemicals cycle within ecosystems Earth is essentially not accumlating or losing mass. Therefore, matter must be recycled and used over and over by organisms. The molecules your are made of were part of countless other organisms in the past and will be part of countless others in the future. Energy flows through ecosystems Energy enters for the most part as light from the sun with the exception of rare deep sea thermal vent and cave ecosystems. The energy of the light is captured during photosynthesis and stored in the electrons of organic molecules. As energy is transferred between molecules, a small amount is lost with each transfer. Eventually all the energy is lost as infrared light to outer space. Therefore energy must always be coming into ecosystem. Fig. 1.4 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How organisms connected to the environment? How are is are all life AIM: What theunited? major characteristics of life? Explain how the atoms that make up me could be part of you at some point in the future. Explain why the energy stored within me will likely never be used by your cells to build/maintain/repair you. Fig. 1.4 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 1. Organization Reductionism vs Holism View Points Reductionism Complex systems like an ecosystem can be explained by its simpler / fundamental components like DNA in combination with the basic laws of chemistry, physics, etc… Holism A complex system cannot be explained by its simpler parts alone. It must be looked at as a whole [“The whole is greater than the sum of its parts” – Aristotle, emergent properties] Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 2. Regulation (homeostasis) Homeostasis is maintaining a stable internal environment conducive to life. Examples: 1. Body Temperature - Get too hot, sweat; get too cold, shiver 2. Blood glucose levels - Glucose too high, insulin secreted from pancreas telling liver and muscle cells to take up glucose from blood and store as glycogen. - Glucose too low, glucagon secreted from pancreas telling liver to break down glycogen and release glucose into blood. 3. Number of red blood cells Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 2. Regulation (homeostasis) Remember that homeostasis does not maintain equilibrium, but in fact prevents equilibrium as the conditions required to maintain life are not those of equilibrium. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 2. Regulation (homeostasis) Example? Glucose levels in the blood – eat a candy bar and blood glucose levels rise above the homeostatic level of 90mg/dl. Consistent high glucose levels denature proteins and in turn damage cells, the result of diabetes. The pancreas (endocrine gland) senses the rise in glucose and secretes the hormone insulin. Hormones are chemicals secreted directly into the blood and talk to other cells (bind protein receptors). Insulin receptors are located on the surface of muscle cells (myocytes – myo = muscle, cyte = cell), fat cells (adipocytes) and liver cells (hepatocytes). These cells are triggered to take up glucose and store it as glycogen (polymer of glucose) in the smooth ER Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 2. Regulation (homeostasis) Negative Feedback is one way and often used to maintain homeostasis A basic means of maintaining a certain concentration or level in a system like the body where the product or output shuts its own production off. Classic example – Thermostat: 1. Room is cold 2. Thermostat sends signal to furnace to generate heat 3. Heat (output) added to the room 4. Heat shuts thermostat off (negative feedback) at a set temperature like 72 degree F thereby shutting its own production off, regulating the temperature of the room Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 2. Regulation (homeostasis) Negative Feedback A General Biological Example: Three enzymes [proteins that catalyze reactions] (enzyme 1, 2 and 3) convert molecules A into molecule D (the output). Molecule D then binds to enzyme 1, shutting it off, which in turn shuts its own production off. This maintains/regulates a specific level of molecule D. Figure 1.11 Negative feedback Enzyme 1 B A Enzyme 1 B Enzyme 2 C C Enzyme 3 D D D D D D D D D D D Chapter Introduction: The Life Study of Life Chapter 51--Energy and the Exploring Cell Scientific Howare is are all life AIM: How enzymes regulated (controlled)? AIM: What theunited? major characteristics of life? Positive Feedback Opposite of negative feedback. When the output of a system goes back (feeds back) and further enhances its own production (in the positive direction) leading to more output and in turn more enhancement and even more output, etc… Such a condition is considered unstable and does not maintain homeostasis… Chapter Introduction: The Life Study of Life Chapter 51--Energy and the Exploring Cell Scientific Howare is are all life AIM: How enzymes regulated (controlled)? AIM: What theunited? major characteristics of life? Positive Feedback Examples: 1. Hypothetical – if your house thermostat worked based on positive feedback, the output (heat) would further activate the thermostat, which would instruct the release of more heat, which would even further activate thermostat, etc… 2. Child Birth contractions – the hormone oxytocin stimulates contration of the uterus. This will cause the baby to press up against the uterus, which causes more oxytocin release, more contractions, more pushing of the baby, more oxytocin, even harder contractions, etc… until baby and placenta are out and Chapter Introduction: The Life Study of Life Chapter 51--Energy and the Exploring Cell Scientific Howare is are all life AIM: How enzymes regulated (controlled)? AIM: What theunited? major characteristics of life? Positive Feedback Examples: 3. Sneezing - You initially have to somewhat sneeze, which causes you to feel more like you have to sneeze, and then more, and more, and aaaaaaahhhhhhh choooooooooooooo! Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 2. Regulation Positive Feedback (homeostasis) W General Biological Example: Three enzymes [proteins that catalyze reactions] (enzyme 4, 5 and 6) convert molecule W into molecule Z (the output). Molecule Z then binds to enzyme 5, speeding it up, causing more molecule Z to be produced. W Enzyme 4 Positive feedback X X Enzyme 5 Enzyme 5 Y Y Enzyme 6 Z Enzyme 6 Z Z Z Figure 1.12 Enzyme 4 Z Z Z Z Z Z Z Z Z Z Z Z Z Z Z Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 3. Growth and Development After fertilization (sperm and egg also known as gametes/sex cells fuse), you underwent mitosis (one cell divides into two genetically identical cells), many times as you grew. Your original cells, called stem cells, differentiated (turned into) all the different types of cells that make up you by having their genes turned on and off** (differential gene expression) in order to grow/develop. **Your eyes cells do not make insulin and therefore this gene is turned off. ALL of your cells have the same DNA, what is different is gene expression (which genes are on and their protein being made). Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 4. Energy Utilization What is energy? An object has energy when it is able to accelerate (speed up or slow down) itself or another object. We obviously accelerate ourselves around and move around other objects. Therefore we use energy…where do we get energy from**? We are heterotrophs (eat others) and must eat plants, which are autotrophs (organisms that make their own food) or we eat animals that have eaten autotrophs. The organic molecules autotrophs contain, like fat (triglycerides) and sugar (glucose/sucrose), have energy…how did these molecules get energy? **Energy can’t be created or destroyed (1st law of thermodynamics). You can’t magically give something the ability to move objects. It must get this ability from somewhere. Ex. A ball on the ground will not be able to move another ball next to it unless… Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 4. Energy Utilization How did these molecules get energy? Most autotrophs – also known as producers because they produce their own food and the food for others, like plants, do photosynthesis – make organic molecules** that have energy, like glucose, from inorganic ones (CO2 and H2O) that do not have readily accessible energy. The energy put into CO2 and H2O to rearrange them into glucose is from sunlight!! **Organic molecules are molecules that contain BOTH carbon and hydrogen Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 4. Energy Utilization How do we get the energy from these molecules? We “burn” them similar to burning a piece of wood, but we do it slowly using many, many chemical reactions known as… cellular respiration. What is the name we give to ALL the chemical reactions in an organism including those involved in cellular respiration? metabolism Examples of chemical reactions (metabolism): 1. Cellular respiration 2. Photosynthesis 3. Protein synthesis by the ribosome 4. Glycogen synthesis and breakdown by the liver 5. Cholesterol synthesis Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 4. Energy Utilization What is a chemical reaction? When chemicals called reactants are rearranged into new chemicals called products. In the above example, carbon dioxide and water are the reactants. They are rearranged (bonds broken and reformed) into the products glucose, oxygen and water by proteins called enzymes that speed up chemical reactions. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 5. Response to the Environment This one should be obvious. ALL organisms do science and collect data about their surroundings. We use the five senses. Based on this data, the organism will react. Example: You are in the street and a car is heading at you resulting in you moving out of the way. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 6. Reproduction All organisms must reproduce either sexually* or asexually** in order to maintain the species. This is the only characteristic NOT required by the individual organism itself to survive. *sexual reproduction – two parents, each contributes half their DNA (sperm and egg) to the offspring resulting in very different DNA in the offspring compared to each parent (genetic diversity) **asexual reproduction – (prefix a- means without) one parent, genetically identical offspring, little genetic diversity – offspring known as CLONES Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 7. Evolution The DNA of populations (population = smallest unit that can evolve) will change over time via… microevolution (genetic drift, gene flow, mutation, natural selection, and sexual selection) resulting in the structure/function of the species changing over time and potentially new species arising. Why is it important that a species maintains genetic diversity? Because the environment is always changing, selecting for/against certain combinations of DNA (organisms). Species need to be able to keep up with the environmental changes. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 7. Evolution Charles Darwin did not figure out the Theory of Evolution (or descent with modification as he called it), he figured out one way in which evolution works… The Theory of Natural Selection Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 7. Evolution The Theory of Natural Selection Nature acts like a filter and only “allows” certain organisms to survive and reproduce. The DNA (genes) of these organisms get passed on and the genes of the others that do not survive and reproduce get erased. Only those organisms best suited to the current environment make it… Will we all survive? Will we all reproduce? Will we have the same number of offspring? Think about what is happening to you right now. Fig. 1.21 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 7. Evolution Population of organisms The Theory of Natural Selection Remember: Populations have Individual Variation already. Populations will always reproduce beyond their capacity (overproduction) resulting in competition. Certain individuals will be better adapted to the environment than others resulting in unequal reproductive success. Adaptations are characteristics in the population that vary like beak size. Successful traits will become more Figure 1.20 frequent in the population adapting the population = evolutionary adaptation. Hereditary variations Overproduction and struggle for existence Differences in reproductive success Evolution of adaptations in the population Fig. 1.20 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 7. Evolution The Theory of Natural Selection Examples: 1. There are humans who cannot get HIV. These people were here before HIV. They did NOT arise because of HIV. 2. Likewise, there are bacteria that are resistant to antibiotics just by chance. The antibiotics did not cause them to arise, it just SELECTS for them. Fig. 1.21 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? 7. Evolution The Theory of Natural Selection “Survival of the fittest” Who is the “fittest”? The fittest organism is the one who passes the most of his/her genes into the next generation (you can say the most offspring, but its more than that...) Who is more fit, a person who lives to 95 years old with two offspring or a person who lives to 22 with six offspring? The 22 year old…more offspring. Fig. 1.21 Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? Living things Life Characteristics exhibit all 7 1. Organization characteristics 2. Regulation 3. Growth and Development 4. Energy Utilization 5. Response to Environment 6. Reproduction 7. Evolution Chapter 1 - Introduction: Exploring The Scientific Life Study of Life How is are all life AIM: What theunited? major characteristics of life? Fig. 1.2 Chapter 1 - Introduction: Exploring Life NEW AIM: How is all life united? Unity in Diversity Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Unity in Diversity There are currently ~2,000,000 known species with an estimated 30,000,000 species on Earth and guess what… We are all essentially the same We are all composed of cells that have DNA, which code for RNA/proteins, which build us, etc… Chapter 1 - Introduction: Exploring The Scientific Life Study of Life NEW How AIM: AIM:isHow all life is all united? life united? Unity in Diversity How is it that all life is so similar? time Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How is all life united? All life is connected by a common ancestor (LUCA- last universal common ancestor) that existed approximately 3.6 billion years ago. That is, if I take any two organisms on this planet right now and look at their parents and their parents parents (grandparents), etc… and keep doing this at some point I will find that both organisms have the same great, great, great, great, etc… grandparents. We are all related. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How is all life united? prokaryotes time eukaryotes Phylogenetic tre Phylo denotes tribe genetics implies birth A diagram showing the birth of all life and its evolutionary relatedness. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How is all life united? prokaryotes time eukaryotes Phylogenetic tre Phylo denotes tribe genetics implies birth According to the diagram, what did we as animals evolve Plants, Fungi and Animals each evolved from a different from? type of protist. Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How is all life united? How do we build these trees and classify organisms (taxonomy*)? They are built based on evolutionary relationships, which are determined by collecting structural and molecular data. *Taxonomy is the classification of life into groups based on evolutionary history using structural (fossils of extinct organisms, bone structure, etc…) and molecular data (DNA, protein sequences, etc…) Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How is all life united? Homologous structures – similar structure, different functions Structural evidence (ex. comparative anatomy) Species with a recent common ancestor usually have similar structures. For example, the bone structure of our arm is more similar to a bat wing than a butterfly wing indicating that we have a more recent common ancestor with bats. Structure can be misleading sometimes and the stronger evidence is… Chapter 1 - Introduction: Exploring The Scientific Life Study of Life AIM: How is all life united? Molecular evidence We can look at proteins (above) or DNA and compare them between species. If two species have very similar DNA then they likely have a very recent common ancestor. The more similar the more recent the common ancestor. *Much stronger than structural as evolution is a change in DNA over time. Different DNA can code for similar structures in certain cases. Chapter 1 Introduction: Exploring Life AIM: How is all life united? Another phylogenetic tree. Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Using this data, how have we grouped organisms? Chapter 1 - Introduction: Exploring Life AIM: How is all life united? The Five Kingdoms No nucleus or other INTERNAL membranous organelles ALL have Nucleus and other INTERNAL MEMBRANOUS ORGANELLES Chapter 1 - Introduction: Exploring Life AIM: How is all life united? The Five Kingdoms No nucleus or other INTERNAL membranous organelles ALL have Nucleus and other INTERNAL MEMBRANOUS ORGANELLES Chapter 1 - Introduction: Exploring Life AIM: How is all life united? prokaryotes Science is tentative!! eukaryotes 6 kingdoms??? Chapter 1 - Introduction: Exploring Life AIM: How is all life united? prokaryotes eukaryotes 6 kingdoms??? Chapter 1 - Introduction: Exploring Life AIM: How is all life united? prokaryotes eukaryotes 3 domains Fig 15.14B Check out http://en.wikipedia.org/wiki/Kingdom_%28biology%29 for a history Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Life’s three domains Bacteria are the most diverse 4 µm and widespread prokaryotes and are now divided among multiple kingdoms. Each of the rod-shaped structures in this photo is a bacterial cell. DOMAIN ARCHAEA Figure 1.15 Many of the prokaryotes known 0.5 µm as archaea live in Earth‘s extreme environments, such as salty lakes and boiling hot springs. Domain Archaea includes multiple kingdoms. The photo shows a colony composed of many cells. Protists (multiple kingdoms) 100 µm are unicellular eukaryotes and their relatively simple multicellular relatives.Pictured here is an assortment of protists inhabiting pond water. Scientists are currently debating how to split the protists into several kingdoms that better represent evolution and diversity. Kingdom Plantae consists of multicellula eukaryotes that carry out photosynthesis, the conversion of light energy to food. Kindom Fungi is defined in part by the nutritional mode of its members, such as this mushroom, which absorb nutrientsafter decomposing organic material. Kindom Animalia consists of multicellular eukaryotes that ingest other organisms. Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Animal phyla kingdoms domains Chapter 1 - Introduction: Exploring Life AIM: How do we organize the diversity of life? 9 Major Animal Phyla Chapter 1 - Introduction: Exploring Life AIM: How do we organize the diversity of life? 1. Porifera 2. Cnidaria 3. Platyhelminthes 4. Nematoda 5. Annelida 6. Mollusca 7. Arthropoda 8. Echinodermata 9. Chordata 9 Major Phyla (under animalia) Chapter 1 - Introduction: Exploring Life AIM: How is all life united? King Phillip Came Over For Grape Soda (mnemonic) Kingdom, phylum, class, order, family, genus, species Most inclusive Least inclusive Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Least inclusive Species Genus Family Order Class Phylum Kingdom Domain Ursus americanus (American black bear) Ursus Ursidae • Classifying life Carnivora Mammalia Chordata Animalia Figure 1.14 Eukarya Most inclusive Chapter 1 - Introduction: Exploring Life AIM: How is all life united? I. Human classification 1. Domain - Eukarya 2. Kingom Animalia 3. Phylum Chordata a) Gill pouches some time in development b) A tail at some time during development c) A stiff notochord d) Thickened set of nerves down the back 4. Subphylum Vertebrata 5. Class Mammalia a) Hair b) Nurse young 6. Order Primata a) Grasping fingers and toes b) Eyes in the front of the head c) Large brain d) Fingernails 7. Family Hominidae a) Primates that walk erect with straight-knee bipedalism b) We are the only living member of this family c) Many ancestral forms 8. Genus Homo a) HUGE brains 9. Species: Homo sapiens Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Modern day species Branch points are speciation events Ancestoral species Phylogenetic Tree Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Chimpanzees are most closely related to old world monkeys, Orangutans or New world monkeys? Explain. Orangutans as their most recent common ancestor was 16 million years ago (Ma). The other two species are 25 and 40 Ma. Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Why is Proconsui not up with the other species on the tree? Remember that moving upward is time. This species went extinct. Chapter 1 - Introduction: Exploring Life AIM: How is all life united? QUESTION After working for months in the lab you finally have the information you need to build a simple phylogenetic tree showing the relationship of six species (A, B, C, D, E, and F). You determined that the DNA sequence of A was most similar to that of C and E, and that the DNA sequence of B was most similar to F. After closer examination, however, you determined that the DNA sequence of C was slightly more similar to A than to E. Species D had a DNA sequence with equal similarity to all the other organisms. Draw a phylogenetic tree showing the evolutionary relationship of these six species. Chapter 1 Introduction: Exploring Life AIM: How do we organize the diversity of life? Chapter 1 Introduction: Exploring Life AIM: How do we organize the diversity of life? http://evolution.berkeley.edu/evosite/evo101/IIAFamilytree.shtml Chapter 1 - Introduction: Exploring Life NEW AIM: What is Science? What is Science Science is something that you do. It is a verb. It’s the process we use to try and know something. It is the only way to try to know something. Chapter 1 - Introduction: Exploring Life AIM: What is Science? How do we do science ? Chapter 1 - Introduction: Exploring Life AIM: What is Science? You need to simply make observations. This is easier said than done in most cases. For example, yes, if you want to know how many bottles are recycled per day in school you can just count them at the end of the day. A simple observation. However, if you want to know how a certain protein binds to DNA and turns on a gene you obviously can’t just look at it. Making observations is one of the most difficult parts of science!! Chapter 1 - Introduction: Exploring Life AIM: What is Science? You need to simply make observations. Hypothetical Observation: You observe that over the past year you have not gotten sick. Not a single cold, which is very uncommon for you. Chapter 1 - Introduction: Exploring Life AIM: What is Science? You need to simply make observations. Hypothetical Observation: You observe that over the past year you have not gotten sick. Not a single cold, which is very uncommon for you. This should lead to a question…why have I not gotten sick this year? Chapter 1 - Introduction: Exploring Life AIM: What is Science? You need to simply make observations. Hypothetical Observation: You observe that over the past year you have not gotten sick. Not a single cold, which is very uncommon for you. This should lead to a question…why have I not gotten sick this year? Perhaps it is that new tea I have been drinking made by heating the leaves of two plants, Camellia japonica and Lobelia chinensis in water at 100 degrees C for two minutes. … Chapter 1 - Introduction: Exploring Life AIM: What is Science? You need to simply make observations. Hypothetical Observation: You observe that over the past year you have not gotten sick. Not a single cold, which is very uncommon for you. This should lead to a question…why have I not gotten sick this year? Perhaps it is that new tea I have been drinking made by heating the leaves of two plants, Camellia japonica and Lobelia chinensis in water at 100 degrees C for two minutes. … If the leaf extract is medicinal in nature then I should be able to give it to other people and they should also become ill less often... Chapter 1 - Introduction: Exploring Life AIM: What is Science? You need to simply make observations. Controlled Experiment An experiment is simply a way of making more observations to see if your hypothesis is supported or refuted. Design the experiment… Make sure you indicate the independent variable, dependent variable, control group, experimental group, the variables you will try to control, etc… Chapter 1 - Introduction: Exploring Life AIM: How is all life united? Things to keep in mind: 1. A hypothesis is NOT and IF/THEN. It is a statement that answers your question like: The plants getting fertilizer will grow taller. NOT if the plants get fertilizer then they will grow taller. 2. The independent variable is the one you alter (it starts with the letter “I” = “I alter”. It is not fertilizer, it is the AMOUNT OF fertilizer. – goes on x axis of graph 3. The dependent variable is what you measure…height, mass, color, etc… - goes on y axis of graph 4. Make sure you use a placebo (sugar pill) if you are treating HUMAN subject only. 5. Always maximize sample size, use a control group for comparison if necessary, and the experiment must be repeatable to be VALID. Chapter 1 - Introduction: Exploring Life AIM: What is Science? There are two possible outcomes of your new observations obtained by doing the controlled experiment: Let’s say you observe that 68% of the people receiving the extract got sick fewer times than the control group over the first year of the study…what does this tell you? This is of course known as the scientific method. Chapter 1 - Introduction: Exploring Life AIM: What is Science? A Case Study in Scientific Inquiry: Investigating Mimicry in Snake Populations • In mimicry – A harmless species resembles a harmful species Flower fly (non-stinging) Honeybee (stinging) Figure 1.26 • In this case study – Mimicry in king snakes is examined – The hypothesis predicts that predators in non–coral snake areas will attack king snakes more frequently than will predators that live where coral snakes are present Scarlet king snake Key Range of scarlet king snake Range of eastern coral snake North Carolina South Carolina Eastern coral snake Figure 1.27 Scarlet king snake Field Experiments with Artificial Snakes • To test this mimicry hypothesis – Researchers made hundreds of artificial snakes, an experimental group resembling king snakes and a control group of plain brown snakes (a) Artificial king snake Figure 1.28 (b) Brown artificial snake that has been attacked • After a given period of time – The researchers collected data that fit a key prediction Key Key % of attacks on artificial king snakes % of attacks on brown artificial snakes Field site with artificial snakes 17% In areas where coral snakes were absent, most attacks were on artificial king snakes 83% X X X North X Carolina XX South X Carolina X X XX XXX 16% 84% Figure 1.29 In areas where coral snakes were present, most attacks were on artificial brown snakes Chapter 1 - Introduction: Exploring Life AIM: What is Science? Tentative and Fallible The findings of science are based on observation, but the observations could be misleading. For example, the Earth appears to be flat… Likewise, people thought Newton’s law of gravity was correct, but Einstein came along and revised it. This type of thing happens all the time. In addition, it was originally thought that water was HO. All of the observations pointed to HO at the time… The more reliable observations you make, the closer one gets to knowing the truth… Chapter 1 - Introduction: Exploring Life AIM: What is Science? What do scientists do with their findings? They publish them in scientific journals like Science, Nature, Cell, EMBO, PLOS One, etc… Chapter 1 - Introduction: Exploring Life AIM: What is Science? We are always doing science! Because you are always making observations, predictions, etc… When you walk down the hall you are collected data (visual data with your eyes, auditory data with your ears, etc…). With this data you generate questions and make predictions. For example, you observe someone running at you. Hypothesis: The person will run into me. Prediction: If I do not move then I will get hit. Experiment: Stay put. Results: The person hit me supporting my hypothesis. Chapter 1 - Introduction: Exploring Life AIM: What is Science? Theory 1. Very broad in nature (describe many, many different phenomenon). 2. Supported by enormous numbers of observations 3. Examples: Theory of Evolution Cell Theory Law 1. Very concise in nature (describes a very specific phenomenon). 2. Supported by enormous numbers of observations 3. Examples: Law of Gravity Laws of thermodynamics Laws of motion Chapter 1 - Introduction: Exploring Life AIM: What is Science? GOD and Science Chapter 1 - Introduction: Exploring Life AIM: What is Science? So is this class really about science ? Chapter 1 - Introduction: Exploring Life Overview Eleven themes that unify biology Table 1.1