Bio 414LS Week 2
Lecture:
Normal Fly
Development
& Microscopy
Lecture:
Fly Genetics,
Husbandry &
Handling
Activity:
Disc dissection
Activity:
Virgining
The lecture and activity are to achieve these objectives:
*Students will be able to briefly describe how a fertilized egg
will develop into a 1st instar larvae with regard to patterning,
germ layers & germ cells.
*Students will be able to describe the life cycle with regard to
timing of stages.
*Students will be able to identify the parts of an adult wing.
*Students will be able to dissect and identify larval imaginal
discs by shape and position.
Assessment:
Sabotage!
Reading:
Tyler Manual
Chapter 8
Drosophila
Guide
Reading:
Fly Pushing
Chapter 1
Activity:
Virgining
Activity:
Virgining
Activity:
Virgining
The lecture and activity are to achieve these objectives:
*Students will be able to explain the relationship between genome sequence,
genetic & cytological maps across the chromosome.
*Students will be able to predict (in a very, very rough way) where their
mutation lies on the genome by comparing the known genetic and/or
cytological map position for their gene.
*Students will be able to describe what an adult fly would look like given a
genotype.
*Students will be able to explain the differences between Drosophila and
human dosage compensation
*Students will be able to explain what balancers are used for and which is used
for which chromosome.
*Students will be able to sort and show the difference between sexes &
virgins.
*Students will be able to set up the light source & dissecting microscope.
Formative Assessment
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Bio 414LS Week 2
Lecture:
Normal Fly
Development
& Microscopy
Lecture:
Fly Genetics,
Husbandry &
Handling
Activity:
Disc dissection
Activity:
Virgining
The lecture and activity are to achieve these objectives:
*Students will be able to briefly describe how a fertilized egg
will develop into a 1st instar larvae with regard to patterning,
germ layers & germ cells.
*Students will be able to describe the life cycle with regard to
timing of stages.
*Students will be able to identify the parts of an adult wing.
*Students will be able to dissect and identify larval imaginal
discs by shape and position.
Assessment:
Sabotage!
Reading:
Tyler Manual
Chapter 8
Drosophila
Guide
Reading:
Fly Pushing
Chapter 1
Activity:
Virgining
Activity:
Virgining
Activity:
Virgining
The lecture and activity are to achieve these objectives:
*Students will be able to explain the relationship between genome
sequence, genetic & cytological maps across the chromosome.
*Students will be able to predict (in a very, very rough way) where their
mutation lies on the genome by comparing the known genetic and/or
cytological map position for their gene.
*Students will be able to describe what an adult fly would look like given a
genotype.
*Students will be able to explain the differences between Drosophila and
human dosage compensation
*Students will be able to explain what balancers are used for and which is used
for which chromosome.
*Students will be able to sort and show the difference between sexes &
virgins.
*Students will be able to set up the light source & dissecting microscope.
Summative Assessment
Big course objective to match:
Students will be able to design an experiment to identify a mutation by mapping
techniques and illustrate how that mutation in the genome could affect a protein and
how that change could cause a phenotype. (Synthesis & Application)
E. (8 pts) Write out the progeny of the crosses for virgin females of one of the
deficiencies above crossed to males of Bloomington stock 1686 (D1 red1 e1/TM3, Sb1)
and describe what the phenotype of males and females of each of the progeny
classes would be regarding whether they have a red tubule phenotype, eye color,
wing morphology, bristle morphology, body color etc. assuming red DOES lie within
the breakpoints of the deletions.