AP Biology- Cellular Respiration
Virtual Lab Alternative Assignment
Name:____________________________________________Period:_____________DUE: ______________
Go to your online book Click STUDY AREA  Select Ch 9 from the drop down box Click GO In the
black left hand margin click on LAB MEDIA Click LabBench Select 5 CELL RESPIRATION
1. In this lab activity:
a) You will observe __________________________________________________________________
b) You will investigate ________________________________________________________________
2. Key Concepts
a. Write the equation for cellular respiration:
3. Design of the Experiment
a. What are the three ways in which you can measure the rate of cellular respiration?
i.
ii.
iii.
b. Sketch a respirometer and label its important features.
c. As the organism inside the respirometer consumes oxygen, what happens to the water?
d. What happens to the CO2 that the organism produces?
e. What rules do you follow to help you read a pipette? What is the measurement of the example
provided?
Assembling the Respirometer
Read the information on this page. Fill out the table
Vial 1
Vial 2
Vial 3
Vial 4
Vial 5
Vial 6
Contents
Temperature
f. How do you ensure that each vial has an equal volume?
g. What is the purpose of the vial with only glass beads?
h. Explain the formula of the gas law. How can you accommodate for the differences in temperature?
4. Analysis of Results
a. What is the equation to determine the rate of respiration? Identify and explain each part.
b. Read the respirometers and determine the rate of respiration. Show your calculations
5. Lab Quiz
a. Which of the following is a true statement based on the data?
b. What is the rate of oxygen consumption in germinating corn at 12oC? SHOW CALCULATION
c. Which of the following conclusions is supported by the data?
d. What is the role of KOH in this experiment?
Extension (You do not need the computer to finish this section)
A cricket is placed in a respirometer and data taken at three
temperatures. The following table shows the data collected.
Temperatures
Time (min)
10 degrees
18 degrees 25 degrees
0
0.0
0.0
0.0
5
0.25
0.6
0.9
10
0.5
0.9
1.4
15
0.7
1.2
1.8
20
0.9
1.6
2.4
a ) Graph the data
(Remember title & axis labels)
b.) Determine the rate of
respiration for each of the
three temperatures.
(Show work!)
ANALYSIS QUESTIONS
The following questions must be discussed in complete sentences for full credit. Please use a separate
sheet of paper and attach to your lab when complete. It may be typed or hand written.
1. This activity uses a number of controls. Identify a control in this experiment and describe its purpose.
2. Identify the independent variable and dependent variable in this lab & explain why you identified each as this
3. Describe and explain the relationship between the amount of oxygen consumed and time.
4. From the slope of the lines on the graph above, determine the rate of oxygen consumption of germinating
and dry peas during the experiments at room temperature and 12 C. Recall that rate = delta Y/delta X. SHOW
WORK. Would you conclude that the non germinating seeds respire?
5. Describe the relationship you see in the slopes of the data. What trends do you see? Why?
6. If you heat the water 5 C higher than your high temperature, what would you predict to be
the peas respiration?
7. What do you think is the highest reasonable temperature that the pea could withstand?
Completely discuss the rationale behind your answer.
8. Why is it necessary to compare the readings from the peas with the readings from the beads?
9. Why did the vial have to be completely sealed around the stopper?
10. Explain why water moved into the respirometer pipettes.
11. If you used the same experimental design to compare the rates of respiration of a 25 g. reptile
and a 25 g. mammal, what results would you expect/ Explain your reasoning.
12. If respiration in a small mammal were studied at both room temperature (21 C) and 10 C,
what results would you predict? Explain your reasoning.
13. Describe the relationship between temperature and consumption of oxygen (O2)