Chapter 19
Oxidative Phosphorylation
Part 2
Oxidative Phosphorylation Part 2
Key Topics: To Know
1.
2.
3.
4.
5.
6.
7.
How cells deal with reactive oxygen species (ROS).
Calculating ΔGo’ of the Proton Motive Force.
Membrane ATPase and how it works.
Cytoplasmic NADH getting into the mitochondria.
Adenylate Control.
Mitochondia and apoptosis.
PMF can be used to ?
ROS : Reactive
Oxygen Species
ΔG of the Proton Motive Force
Nobel Prize for Chemiosmotic Model = PMF
Converting PMF to ATP
Mitochondrial Respiration+Phosphorylation
EOC Problem 9: Compartmentalization of Citric Acid Cycle.
Adjusting the pH Generates ATP
Molecules that Collapse the PMF
DNP is an Uncoupler
EOC Problem 6: Figuring out what happens with
uncouplers.
Odorous Flowers that Heat Up
Using Uncoupled Respiration
Worked out F1 ATPase
kinetic mechanism +
First purified the FoF1
ATPase
ATP Synthase
ATP Synthase Catalyzes by Steps
F1 Fo ATP synthase
F1 Complex
Used to Inhibit ATP Synthase Reaction
F1 ATPase Spinning
ATP Synthase Seen Rotating !
ATP Synthase Substrate Transport
ADP and Pi are
products of
Anabolism
EOC Problem 11
is the effect of
ADP and Pi on
ATP Synthesis.
Get Ready, NADH
formed in
Cytoplasm in Next
Glycolysis NADH Enters the Mitochondria by the Malate Aspartate Shuttle
NADH from Glycolysis Can Also Get in by Glycerol Phosphate DH
Also has a
Anabolic Role
EOC Problems 13 and 14: Get in to the Pasteur Effect and
“Petites” in Yeast Colonies. Fun stuff.
Adenylate Control of
Glycolysis/CAC/e-transport
1. Acceptor Control:
availability of ADP + Pi
2. Mass action ratio:
[ATP]/([ADP] [Pi])
EOC Problem 17 Gets into
the rate of ATP turnover in
heart muscle…the muscle
that always has to be “on”.
EOC Problem 18 Gets into
the same in insect flight
muscle.
Inhibitory Protein IF1 Stops Loss of ATP During
Ischemia that is Hypoxia
IF (red and white) forms dimers at pH 6.5 to Stop Rotation of
ATP Synthase
Thermogenin – Protein Uncoupler in Brown Fat
Mitochondria in Adrenal Gland: P-450 Oxygenases
Specialized for Steroid Synthesis
Electron Flow to P-450
Stress,
Damage
Apoptosis is
Regulated by the
Mitochondria
EOC Problem 19:
relates
mitochondrial
function to cancer.
Hint think about the
first couple of
slides.
Initiates a series of
proteasess
Escherichia coli Electron Transport
Bacterial Quinone
Bacteria Use the PMF to Rotate Their Flagella
Proton Motive Force Functions To
1. Synthesize ATP (from ADP + Pi).
2. Active Transport (Symports, Antiports, Uniports; review
Chapter 11).
3. Rotate Bacterial Flagella.
4. Reversed Electron Transport (some Photosynthetic and
Chemoautotrophic Bacteria)
Things to Know and Do Before Class
1. How cells deal with reactive oxygen species (ROS).
2. Calculating ΔGo’ of the Proton Motive Force.
3. Membrane ATPase and how it works.
4. Cytoplasmic NADH getting into the mitochondria.
5. Adenylate Control.
6. Mitochondria and apoptosis.
7. PMF can be used to ?
8. EOC Problems: 6, 9, 11,13,14, 17, 18, 19.