Cells The smallest unit of life Which of these is the smallest object? 20% 1. 2. 3. 4. 5. 20% 20% 20% 3 4 20% Human cell Protein molecule Virus Bacteria Amino acid 1 2 5 Prokaryotic organisms lack a nucleus in their cells. Which of these organisms is prokaryotic? 25% 25% 25% 2 3 25% 1. Plants 2. Fungi 3. Protists (such as Amoeba and Paramecium) 4. Bacteria 1 4 Which of these objects can be seen with the aid of an ordinary student microscope? 25% 1. 2. 3. 4. 25% 25% 2 3 25% Human cell Virus Protein molecule Oxygen atom 1 4 Which of these is made up of cells? 1. 2. 3. 4. 5. Animals Plants Fungi Bacteria Only animals and plants 6. All of these 17% 1 17% 2 17% 17% 3 4 17% 5 17% 6 Discovering the Cell 1665 - Robert Hooke Anton van Leeuwenhoek late 17th, early 18th centuries • 1839 – Cell Theory is proposed independently by Theodore Schwann and Matthias Jakob Schleiden. • Cell Theory: • The cell is the fundamental structure of all living things. • All living things are made of cells. • Cells arise from pre-existing cells through cell division. Ernst Ruska - 1938 Light micrograph Paramecium Scanning electron micrograph Transmission electron micrograph Cell Membrane Scanning electron micrograph Which of these can be seen with an electron microscope but not a light microscope? 25% 1. 2. 3. 4. 25% 25% 2 3 25% Human cells Bacteria Protein molecules Amino acids 1 4 • Discuss and write down your ideas: • How did the invention of the microscope change the human view of the world? • How did the invention of the electron microscope change our understanding of cells? W O R K T O G E T H E R Prokaryotic Cells Prokaryotic Features • No membrane-bound organelles, such as a nucleus. • DNA in one large ring-shaped chromosome • An enormous variety of metabolic pathways. • Highly successful and adaptable. External structure Coccus (spherical) Spirillus (spiral) Bacillus (rod-shaped) Internal Structure chromosome (nucleoid region) pili ribosomes food granule prokaryotic flagellum capsule or slime layer cell wall plasma membrane cytosol plasmid (DNA) True or False: A virus is a kind of bacteria. 1. True 2. False 50% 1 50% 2 Viruses • A virus is usually not considered living. • A virus consists only of a protein coat (sometimes with a lipid outer layer) and a piece of genetic material (DNA or RNA). Bacteria vs. Virus Features Bacteria Virus Reproduces independently? Yes No Has genetic material? Yes Yes Has cell membrane? Yes No Has metabolism? Yes No Living? Yes No Which of these does a bacteria NOT have? 25% 1. 2. 3. 4. 25% 25% 2 3 25% DNA Cell membrane Nucleus Bacteria have none of these. 1 4 Eukaryotic Cells Eukaryotic Features • Cells contain membrane-bound organelles. • Nucleus holds multiple strands of DNA, which condense into chromosomes during cell division. • May be single-celled or multi-cellular organisms. Animal cell nuclear pore chromatin (DNA) nucleolus nuclear envelope cytosol nucleus centriole rough endoplasmic reticulum plasma membrane lysosome ribosomes on rough ER smooth endoplasmic reticulum free ribosome mitochondrion Figure 4-3 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Golgi apparatus vesicle Nucleus nuclear envelope nucleolus nuclear pores chromatin Endoplasmic Reticulum ribosomes rough ER vesicles smooth ER Golgi Apparatus Protein-carrying vesicles from ER merge with Golgi apparatus Golgi apparatus Vesicles carrying modified protein leave Golgi apparatus ER and Golgi function 5 Vesicles merge with the plasma membrane and release protein by exocytosis. 4 Completed protein is packaged into vesicles. 3 Vesicles fuse with Golgi. The protein may be altered and finished. 2 Protein is packaged into vesicles and travels to Golgi apparatus. 1 A protein is synthesized in the rough ER. A cell that produces a lot of protein must have an extensive: 25% 1. 2. 3. 4. 25% 25% 2 3 25% Nucleus Rough ER Smooth ER Golgi apparatus 1 4 Proteins are packaged for delivery by the: 33% 33% 33% 1. Nucleus 2. Rough ER 3. Golgi apparatus 1 2 3 • Discuss and summarize in your own words the roles of: • the cell nucleus • the rough ER • the Golgi complex W O R K T O G E T H E R Lysosomes ER, Golgi, & Lysosome Function after Phagocytosis plasma membrane 4 Lysosome fuses with food vacuole and enzymes digest food. waste food vacuole lysosome 3 Lysosomes bud from Golgi. Golgi ER 2 Golgi modifies enzymes. digestive enzymes 1 Enzymes are synthesized in ER. nucleus Mitochondrion outer membrane inner membrane matrix cristae intermembrane compartment 0.2 micrometer White blood cells must locate, engulf, and destroy many foreign particles. Which organelle will help them most in this task? 25% 25% 25% 2 3 25% 1. Nucleus 2. Lysosome 3. Endoplasmic reticulum 4. Golgi bodies 1 4 W O R K • Discuss and summarize in your own words the roles of the lysosome and the mitochondrion in animal cell nutrition. T O G E T H E R Animal cell nuclear pore chromatin (DNA) nucleolus nuclear envelope cytosol nucleus centriole rough endoplasmic reticulum plasma membrane lysosome ribosomes on rough ER smooth endoplasmic reticulum free ribosome mitochondrion Figure 4-3 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Golgi apparatus vesicle True or false: Plant cells are much more primitive than animal cells and have far fewer types of organelles. 50% 50% 1. True 2. False 1 2 Plant cells are: 50% 50% 1. Eukaryotic 2. Prokaryotic 1 2 Plant Cell - same organelles as an animal cell, plus a few more plastid mitochondrion choloroplast cytosol Golgi apparatus smooth endoplasmic reticulum central vacuole vesicle plasmodesma rough endoplasmic reticulum cell wall plasma membrane nuclear pore nucleus chromatin (DNA) nucleolus nuclear envelope ribosomes free ribosome Cell Wall secondary cell wall primary cell wall plasma membrane middle lamella W O R K • How is the cell wall of plant cells similar to an animal’s skeleton? How is it different? T O G E T H E R Chloroplast outer membrane inner membrane stroma thylakoid channel interconnecting thylakoids granum (stack of thylakoids) 1 micrometer Leucoplasts and other plastids plastid starch globules 0.5 micrometer What do chloroplasts do? 33% 33% 33% 1. Make energy 2. Use energy to make molecules. 3. Break sugar down to release energy. 1 2 3 W O R K • Chloroplasts carry out photosynthesis to make sugars and other monomers. What does the plant use these monomers for? T O G E T H E R One-celled Eukaryotic organisms have unique challenges. A Paramecium must: ingest food excrete waste sense and respond to the environment (a) Cilium propulsion of fluid power stroke plasma membrane return stroke (b) Flagellum direction of locomotion propulsion of fluid continuous propulsion protein "arms" central pair of microtubules section of cilium (transmission EM) Paramecium plasma membrane basal body 0.1 micrometer cilium contractile vacuole full reservoir contracted reservoir Water enters collecting ducts, fills central reservoir. collecting ducts central reservoir pore Reservoir contracts, expelling water through pore. • In a one-celled organism, which organelle is most like: • A stomach (assists with digestion)? • A kidney (removes excess water)? W O R K T O G E T H E R Recap • Prokaryotic cells have no membranebound organelles. • Eukaryotic cells have membrane-bound organelles, which compartmentalize processes for better efficiency. • ALL cells have a cell membrane and DNA.