Taxonomy • Let’s look at the modern system of taxonomy from the top down. Lessons of Classification • Bacteria and Archaea used to be grouped together in one kingdom called Monera. • Each Kingdom is further divided into Phylum → Class → Order → Family → Genus → Species. • Species is the most specialized level of classification with the most diversity at this level. • When referring to a particular species, a dichotomous name is used that includes the genus name and the species name. • For examples, humans are called Homo sapiens. • Homo is the genus name and sapiens is the species name. Taxonomy “Tip” • A good way to remember the levels of taxonomy is through the use of a mnemonic. • A mnemonic uses the first letters of what you need to remember in a memorable phrase. • In this case, a mnemonic for remembering the system of taxonomy could be: • “King Philip Came Over For Good Spaghetti” representing Kingdom, Phylum, Class, Order, Family, Genus and Species. How to Classify • Taxonomy comes from the Greek word ‘taxis’ which means to order or rank. In this system, each species is using two parts: – the name of the genus to which it is assigned – the "specific name" which identifies the particular species within the genus. • Latin names are assigned to both the genus and species and both words are written in italics. • Classification is used to identify organisms and make our meaning as clear as possible. Kingdom Archeabacteria and Kingdom Eubacteria Common characteristics of Eubacteria and Archeabacteria 1. 2. 3. 4. Cells are prokaryotic. All are single celled. Have single chromosome. Reproduce asexually through binary fission. Archeabacteria • Often live in environments where other organisms wouldn’t survive – Usually anaerobic Three main groups of Archeabacteria 1. Methanogens – produce methane 2. Extreme thermophiles – thrive in temperatures up to 110⁰C 3. Extreme halophiles – live in very salty water Three main groups of Eubacteria • Spherical – coccus/cocci • Rod-shaped – bacillus/bacilli • Spiral – spirillum/spirila • Staphylo – Bunch of grapes • Diplo – Pairs • Strepto – Long chains Typical Structures Eukaryotic Respiration A. Obligate aerobes – must have oxygen to survive. ex. tuberculosis B. Obligate anaerobes – only grow in the absence of oxygen. ex. Live deep in the soil or marine sediment C. Facultative anaerobes – can survive with or without oxygen. ex. Most bacteria Eukaryotic Modes of Nutrition • Heterotrophs – most bacteria; obtain nutrients from other organisms – Parasite: obtaining nutrients without contributing to the health of the of the host (disease causing bacteria – Saprophytes: obtain their nutrients from dead and decomposing matter • Autotrophs – make food from inorganic substances by photosynthesis or chemosynthesis. Asexual Reproduction in Bacteria • Binary Fission (similar to mitosis, but simpler) 1. Single DNA strand replicates. 2. The identical pieces of genetic material are transferred to each new cell. 3. After replication the cell divides and produces a new cell wall. Sexual Reproduction in Bacteria • Conjugation – two bacteria make cell to cell contact and DNA is transferred from one (donor) to the other (recipient). • Not common in bacteria. Endospores • During unfavourable environmental conditions an endospore is formed so that the cell can remain dormant or “at rest”. – They are resistant to heat and can not easily be destroyed. – When suitable conditions return, an active bacterial cell emerges. Protists • The protist kingdom is the most challenging group of organisms to classify. • There are 115 000 species of protists which display great variation. • Protists have characteristics that are similar to organisms found in each of the other kingdoms. Some are unicellular which is similar to bacteria. • However, one distinguishing characteristic that separates bacteria from protists is the presence of a nucleus. • Remember, bacteria are prokaryotic because their DNA is free floating within the cytoplasm. • All protists are eukaryotic which means they contain a distinct nucleus which surrounds their DNA. • This evolutionary development generated the need for a distinct classification for these organisms. • Classifying protists becomes challenging because of their vast differences. • Most protists are unicellular, but there are some that are multicellular. • Some are autotrophs while others feed on organic matter and are heterotrophic. • Scientists have organized protists into three different groups: animal-like protists, plantlike protists, and fungi-like protists. • In all, there are 11 phyla in the protist kingdom. Animal-like Protists (Protozoa) • Protozoans are classified by their type of locomotion, life cycle, reproduction, nutrition and whether the protist is parasitic or free-living. • All animal-like protists are heterotrophs. Similar to organisms in the animal kingdom, these protists have to be motile to search out food. • Protozoans can either absorb pre-digested materials through their cell membrane or they can engulf their food. • Sarcodina, Mastigophora, Ciliophora, and Sporozoa are the four phyla of animal-like protists. You will now learn about each phylum in greater detail. Sarcodina • The amoeba is probably the most famous protist in this phylum. Perhaps you even had the opportunity to view an amoeba under a microscope. • One of the interesting characteristics of protists in this phylum is the way they • The image above shows an move. amoeba moving toward a • By extending and retracting its cytoplasm, an amoeba is group of cells. The movement of the cytoplasm forms the able to create “false feet” pseudopods as the amoeba called pseudopods. moves towards the food. • Pseudopods also enable an amoeba to capture and engulf its food by phagocytosis. • Below is a sequence of pictures showing an amoeba engulfing an algal cell. • In the fourth sequence you will notice the appearance of the pseudopods surrounding the algal cells. Mastigophora • In the phylum Mastigophora, the protists contain one or more flagellum (pl.flagella) for locomotion. • A flagellum resembles a tail and is attached to the cell body of the protozoan. A protist will generate motion when the flagellum begins to rotate. • Most of these protozoans are harmless. • However, Giardia lamblia, a parasitic protozoan causes upset stomach and diarrhea in humans. • This parasite lives in the digestive tract of animals, like cows. • Humans can contract the parasite when they consume food or water that has come in contact with the feces of infected animals. Ciliophora • Cilates contain cilia, tiny hair-like structures, throughout the outer covering of their cell body. • In the image on the right, the cilia appear clear. The dark spot in the centre of the paramecium is the macronucleus which functions like a nucleus. • A micronucleus, not shown, is smaller and is involved in reproduction. • Most paramecium reproduce by binary fission; however, they can also undergo conjugation. Sporozoa • Sporozoans rely on other organisms for movement. They lack cilia, pseudopods, and flagella. Therefore, these protists are all parasitic. • Malaria is caused by Plasmodium. Symptoms include fever, headache, and vomiting: malaria can • Approximately 40% of the quickly become lifeworld's population, mostly those threatening if not treated. living in the world's poorest • Malaria can be spread from countries, are at risk of malaria. person to person and • Every year, more than 500 million through mosquito bites. people become severely ill with malaria. (WHO, May 2007) • Plant-like protists all contain chlorophyll, the pigment found in plant cells, which is involved in the process of photosynthesis. • Most plant-like protists are able to produce their own food. • However, some are heterotrophic and ingest materials from their surrounding. • There are six phyla within the plant-like protist group. Euglenophyta • In the past, euglenids have been classified as both plants and animals because in the presence of light they are photosynthetic. • However, in the absence of light they become heterotrophic. The Euglena is one of the most studied • One feature of the Euglena plant-like protists. which is not shown is its • The image on the right shows flagellum. Most species contain some features of the Euglena. two flagella which allow for • The dark spot on the bottom movement. Similar to animalrepresents the eye spot like protists, the euglenids which is used to detect light. reproduce asexually. Rhodophyta (Red Algae): Phaeophyta (Brown Algae) and Chlorophyta (Green Algae) • The protists in these phyla all contain chloroplast which means they too photosynthesize. • In addition to containing chlorophyll, they also contain other pigments that allow them to absorb additional wavelengths of light. • These algae are multicellular and they generally inhabit moist to wet environments. • Reproduction varies greatly amongst this group. The most common form of reproduction is through binary fission. • However some algae reproduce sexually. • Green algae play an important role in providing oxygen through photosynthesis; algae are also part of the aquatic food chain. • You may have noticed green algae forming in ponds or in shallow rivers and lakes. • Fertilizer run-off may lead to excessive growth of green algae causing an imbalance in the natural environment. Chrysophyta • Diatoms provide much of the nourishment for aquatic organisms. • These protists are unicellular and each species has a very unique shape. • Structurally, they are composed of two halves that fit perfectly together. This is an image of various diatoms showing circular and triangular shapes. • When they reproduce asexually, they split and separate into two. • Then each original part grows into a new half. • After several generations of reproducing asexually, the size of the diatom reduces significantly. • When this occurs, the diatoms reproduce sexually by producing a zygote. • While diatoms do not contain flagella, many other protists in this phylum are flagellated. Pyrrophyta • Have you ever had the opportunity to watch the ocean light up from within its depths? • This phenomenon is the result of dinoflagellates, a protist from this phylum. When water containing large amounts of dinoflagellates becomes agitated, bright flashes of light are visible. • In response to excessive nutrients or elevated water temperatures, dinoflagellates experience rapid growth called a bloom. • In addition to tinting the water red, dinoflagellates produce toxins. • These toxins accumulate in the bodies of fish which can then affect humans who consume the toxic fish. • Dinoflagellates are photosynthetic, but they can also ingest materials from their environment. • They are generally unicellular, contain two flagella and reproduce asexually. Fungus-like Protists • These protists are generally referred to as slime moulds and are classified in the phylum Gymnomycota. • These protists are heterotrophic and generally feed on dead organic materials. • By extending its cytoplasm, • The characteristics of slime similar to an amoeba, slime moulds vary greatly during moulds are able to move and their lifecycle. unite to form multicellular • They begin their life as a masses. These masses shrink single cell. in size and eventually produce spores. Quiz – True or False? 1. Protists can be prokaryotic or eukaryotic. 2. Animal-like protists contain chlorophyll. 3. An amoeba moves by extending its cytoplasm and producing pseudopods. 4. Flagella provide locomotion for protists in the phylum Ciliophora. 5. Parasitic protists are responsible for disease in humans. 6. Plant-like protists have no need to move, therefore do not contain flagella. 7. Euglena is a plant-like protist that is both autotrophic and heterotrophic. 8. Global warming will have an effect on green algae growing in lakes and rivers. 9. Slime moulds show the potential for complex speciation. 10. The Protist kingdom is very diverse. Answers 1. False – The classification of protists is based on the fact that they all contain a nucleus and therefore are eukaryotic. They cannot be classified in the animal or plant kingdom because they lack specialized tissues. 2. False – Only plant-like protists contain chlorophyll. Remember this is the pigment that allows these protists to photosynthesize. 3. True – The false feet produced by the extension of cytoplasm provide mobility to an amoeba. In addition, the pseudopods also allow amoeba to engulf their food by phagocytosis. 4. False – Protists in the phylum Ciliophora contain tiny hair-like structures called cilia that surround their cell body. The motion of these structures provides movement for these protists. 5. True – Parasitic organisms rely on a host for survival. Some protists cause serious illnesses such as malaria and have been responsible for outbreaks like the Irish potato famine in the mid 1800's. 6. False – Many plant-like protists contain flagella including Euglena and dinoflagellates. 7. True – Euglena contain chlorophyll and will photosynthesize when light is available. In dark conditions, Euglena will feed off of organic material. 8. True – Elevated water temperatures increase the growth rate of green algae. This causes an imbalance in the ecosystem. The overgrowth of the algae inhibits the growth and survival of other organisms. 9. True – Unicellular slime moulds uniting to form a multicellular mass illustrates the potential for specialization. Soon, cells may become specialized to carry out a specific task. 10. True – There is great variation in reproduction; from asexual fragmentation to sexual conjugation. Protists can be autotrophic or heterotrophic or a combination of both. Locomotion may involve a flagellum or cilia. Finally, protists may be free living or parasitic.