Laboratory
Safety Rules
While working in the science laboratory,
you will have certain important
responsibilities that do not apply to
_____________
other classrooms. You will be working
with materials and apparatus which, if
handled carelessly or improperly, have
injury or
the potential to cause ______
discomfort to someone else as well as
yourself.
A science laboratory can be a safe place in
which to work if you, the student, are
foresighted, alert, and cautious. Violating
any of the following regulations will result
in you being suspended from class or
permanently removed from the class.
The following practices will be followed:
instructor must be present during the
1. An _________
performance of all laboratory work.
accident to the teacher
2. Report any _________
minor
immediately, no matter how_______,
including reporting any burn, scratch, cut,
or corrosive liquid on skin or clothing.
3. Prepare for each laboratory activity by
reading all instructions before coming to
_______
directions implicitly and
class. Follow all _________
modification
intelligently. Make note of any __________
in procedure given by the instructor.
4. Any science project or individually
planned experiment must be
approved by the teacher.
__________
5. Use only those materials and equipment
authorized by the instructor.
_________
immediately of any
6. Inform the teacher___________
equipment not working properly.
spill on
7. Clean up any nonhazardous _____
immediately
the floor or workspace __________.
Wear Your
Safety Goggles!
Which would you rather read?
FLINN Scientific, Inc "Your Safer Source for Science Supplies" - Poster
• I'm a little Chemist, short and stout,
• Here are my goggles, here are my eyes.
• When I don't wear my goggles,
• my teacher shouts,
• PUT THEM ON OR YOU GET OUT!
eye protection as
8. Wear appropriate ______________,
directed by the instructor, whenever you
are working in the laboratory. Safety
goggles must be worn during hazardous
activities involving caustic/corrosive
_________
chemicals, heating of liquids, and other
activities that may injure the eyes.
9. Splashes and fumes from hazardous
chemicals present a special danger to
contact lenses Therefore,
wearers of _____________.
students should preferably wear regular
glasses (inside splash -proof goggles,
when appropriate) during all class
activities or purchase personal splashproof goggles and wear them whenever
exposure to chemicals or chemical fumes
is possible. Students with open skin
wounds on hands must wear gloves or be
excused from the laboratory activity.
carry hot equipment or
10. Never ______
dangerous chemicals through a ______
group
of students.
labels and equipment
11. Check ______
instructions carefully. Be sure correct
used in the proper manner.
items are ______
chemicals being used
12. Be aware if the _________
are hazardous. Know where the material
MSDS is and what it
safety data sheet (______)
indicates for each of the hazardous
chemicals you are using.
taste anything or touch
13. Never ______
chemicals with the hands, unless
specifically instructed to do so.
__________
14. Test for odor of chemicals only by
waving your hand above the container
______
distance
and sniffing cautiously from a ________.
in the laboratory or
15. Eating or drinking ___
NOT
from laboratory equipment is ____
permitted.
mechanical pipette filler (never
16. Use a __________
the mouth) when measuring or transferring
small quantities of liquid with a pipette.
17. When heating material in a test tube,
look into the tube or point it in
do not _____
the direction of any person during the
process.
reagents back into bottles,
18. Never pour ________
exchange stoppers of bottles, or lay
stoppers on the table.
acids always pour
19. When diluting _____,
water never the reverse.
acids into ______,
Combine the liquids slowly while stirring
to distribute heat buildup throughout the
mixture.
20. Keep _______
hands away from face, eyes,
and clothes while using solutions,
specimens, equipment, or materials in the
laboratory. Wash hands as necessary
conclusion
and wash thoroughly at the __________
of the laboratory period.
21. To treat a burn from an acid or alkali,
wash the affected area ___________
immediately with
plenty of running water. If the eye is
involved, irrigate it at the eyewash station
without interruption for 15 minutes.
Report the incident to your instructor
immediately.
location of the emergency
22. Know the ________
shower and eyewash station, fire blanket,
fire extinguisher, fire alarm box, and exits.
23. Know the proper fire and earthquake
procedures
drill ___________.
wrist
24. Roll long sleeves above the _____.
Long, hanging necklaces, bulky jewelry,
and excessive and bulky clothing should
not be worn in the laboratory.
laboratory
25. Confine long hair during a _________
activity.
cover the toes, rather
26. Wear shoes that _____
than sandals, in the laboratory.
clean Floors and
27. Keep work areas _____.
aisles should be kept clear of equipment
and materials.
burners only as instructed
28. Light gas ________
volatile
by the teacher. Be sure no _______
materials (such as alcohol or acetone) are
being used nearby. Use a burner with
extreme caution. Keep your head and
________
clothing away from the flame and turn it off
when not in use.
29. Use a fire blanket (stop, drop, and roll)
extinguish any flame on a person.
to _________
30. Dispose of laboratory waste as
_________
instructed by the teacher. Use separate,
designated containers (not the
___________)
wastebasket for the following:
Matches, litmus paper, wooden
toothpicks and so on
splints, __________,
_______
Broken and waste glass
Rags, paper towels, or other absorbent
materials used in the cleanup of
flammable solids or liquids
__________
liquids and solids
Hazardous/toxic ______
31. Place books, purses, and such items in
the designated storage area. Take only
laboratory manuals and notebooks into
_________
working area.
the _______
32. Students are not permitted in
storage rooms or teachers'
laboratory _______
workrooms without the approval of the
teacher.
________
33. To cut small diameter glass tubing, use
file or tubing cutter to make a deep
a ____
scratch. Wrap the tubing in a paper towel
before breaking the glass away from you
with your thumbs. Fire polish all ends.
visual
34. Hot and cold glass has the same _____
appearance. Determine whether an
object is hot by bringing the back of your
hand close to the object.
35. Match hole sizes and tubing when
inserting glass tubing into a stopper. If
necessary, expand the hole first by using
an appropriate size cork borer. Lubricate
the stopper hole and glass tubing with
water or glycerin to ease insertion, using
towels to protect the hand. Carefully twist
______
(never push) glass tubing into stopper
holes.
broken glass from the
36. Remove all ________
work area or floor as soon as possible.
Never handle broken glass with bare
hands; use a counter _____
brush and dustpan.
37. Report broken glassware, including
thermometers, to the instructor
immediately.
__________.
38. Operate electrical equipment only in a
dry hands.
dry area and with _____
39. When removing an electrical plug from
plug not the
its socket, pull the ______,
electrical cord.
40. Always approach laboratory
serious and courteous
experiences in a _______
manner.
clean the laboratory area
41. Always ______
before leaving.
42. Students and teacher wash hands with
soap and water before leaving the
______
laboratory area.
43. When heating volatile or flammable
materials, use a water bath; that is, heat
the materials in or over heated water,
using a hot plate to heat the water.
Extinguish all open flames.
_________
caution in using scissors,
44. Exercise _______
scalpels, dissecting needles, and other
sharp-edged instruments. Pass them with
handles extended when handing them to
other persons.
immediately of any
45. Inform the teacher ___________
equipment not working properly.
46. Use the fume hood whenever noxious,
fumes are produced
corrosive, or toxic _______
or released.
clean before
47. Be sure all glassware is ______
use. Clean glassware thoroughly ______
after
use. Residue may cause errors in new
experiments or cause a _______
violent reaction
or explosion.
The following actions will result in
being dropped from the class:
Misuse of safety equipment including, but
_______
not limited to
–eyewash stations
–emergency showers
–fire extinguishers
–fire blanket
Vandalizing school equipment or furniture.
__________
Endangering or causing harm to others.
__________
http://lansce.lanl.gov/training/FST2004/images04/chemicals1.gif
LABORATORY SAFETY
• Chemical Hazard Label
• MSDS
• Safety Quiz
Courtesy Christy Johannesson www.nisd.net/communicationsarts/pages/chem
Safety Symbols
NFPA CHEMICAL HAZARD LABEL
FLAMMABILITY
HEALTH
REACTIVITY
(STABILITY)
SPECIAL
NFPA CHEMICAL HAZARD LABEL
0
4
2
4
3
Least
Serious
4
Flammable vapor which burns
readily
0
Substance is stable
Most
Serious
NFPA CHEMICAL HAZARD LABEL
Burns readily.
Diborane
Severe
health
risk.
4
4
3
W
Avoid water.
May
detonate
with heat or
ignition.
NFPA CHEMICAL HAZARD LABEL
Complete Label for Acetone
NFPA CHEMICAL HAZARD LABEL
Complete Label for Phosphine
MSDS
• Material Safety Data Sheet
• On file for all purchased chemicals.
• Includes all information shown on a
chemical label and more.
• Different formats are used by different
chemical companies.
MSDS
Health Hazard
4
Very short exposure could cause death or serious residual injury
even though prompt medical attention was given.
3
Short exposure could cause serious temporary or residual injury
even though prompt medical attention was given.
2
Intense or continued exposure could cause temporary
incapacitation or possible residual injury unless prompt medical
attention is given.
1
Exposure could cause irritation but only minor residual injury even
if no treatment is given.
0
Exposure under fire conditions would offer no hazard beyond that
of ordinary combustible materials.
FLAMMABILITY
4
Will rapidly or completely vaporize at normal pressure and
temperature, or is readily dispersed in air and will burn readily.
3
Liquids and solids that can be ignited under almost all ambient
conditions.
2
Must be moderately heated or exposed to relatively high
temperature before ignition can occur.
1
Must be preheated before ignition can occur.
0
Materials that will not burn.
1 Prior
to 1996, this section was titled "Reactivity". The name was changed because many people did not understand the distinction
between a "reactive hazard" and the "chemical reactivity" of the material. The numeric ratings and their meanings remain unchanged.
INSTABILITY1
4
Readily capable of detonation or of explosive decomposition or
reaction at normal temperatures and pressures.
3
Capable of detonation or explosive reaction, but requires a strong
initiating source or must be heated under confinement before
initiation, or reacts explosively with water.
2
Normally unstable and readily undergo violent decomposition but
do not detonate. Also: may react violently with water or may form
potentially explosive mixtures with water.
1
Normally stable, but can become unstable at elevated
temperatures and pressures or may react with water with some
release of energy, but not violently.
0
Normally stable, even under fire exposure conditions, and are not
reactive with water.
OX
This denotes an oxidizer, a chemical which can greatly
increase the rate of combustion/fire.
Unusual reactivity with water. This indicates a potential hazard
using water to fight a fire involving this material.
This section is used to denote special hazards. There are only two NFPA 704 approved symbols
ACID
This indicates that the material is an acid, a corrosive material
that has a pH lower than 7.0
ALK
This denotes an alkaline material, also called a base. These
caustic materials have a pH greater than 7.0
This denotes a material that is corrosive (it could be either an
acid or a base).
The skull and crossbones is used to denote a poison or highly
toxic material. See also: CHIP Danger symbols.
The international symbol for radioactivity is used to denote
radioactive hazards; radioactive materials are extremely
hazardous when inhaled.
Indicates an explosive material. This symbol is somewhat
redundant because explosives are easily recognized by their
Instability Rating.