Surface Hydrophobicity Causes SO2 Tolerance in Lichens Hauck et. all Presented by: Sarah Laperriere Ari Nalbandian Purpose Does surface hydrophobicity cause sulfur dioxide resistance? Key Words Hydrophobic (water-fearing) Repels water Hydrophilic (water-loving) Attracted to water Contact Angle Markus Hauck Georg-August University of Göttingen Interests include: Lichen secondary metabolites Biodiversity Global change ecology Long walks on the beach http://www.goettingen.studip.de/extern.php?module=Persondetails&config_id=91362d34fe8a e37349a29787e7d&range_id=2b648cb9a0cf4573c5d4c3d661e6d1df&username=mhauck Sulfur Dioxide (SO2) http://volcanoes.usgs.gov/Imgs/Jpg/GasEffects/GasesChemistry.jpg Why Be Hydrophobic? Thallus saturated with water = less gas exchange = less photosynthesis Hydrophobic thallus exterior prevents thallus from becoming saturated http://www.buffelskloof.info/images/Lichen%20detail.JPG Variables Independent - Lichen SO2 tolerance Dependent - Lichen hydrophobicity Experimental Design 50 lichen species, known low to high SO2 sensitivities 500 m deionized water droplets placed on surface of air-dry thalli Contact angles measured to quantify hydrophobicity Replicated 5 times Lichens extracted with acetone to remove extracellular substances Methods of Tabulation and Evaluation Contact Angle http://www.dsm.com/en_US/html/dep/adhesive_bonding.htm Results Table 2: contact angles of water droplet placed on the thallus surface of lichen species http://aob.oxfordjournals.org/content/101/4/531/T2.ex pansion.html Water droplets placed on the surface of lichen thalli of Bacidina inundata (A), Cetraria islandica (B), Cladonia coniocraea, podetium (C), C. Hauck M et al. Ann Bot 2008;101:531-539 © The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org Data Interpretation “Increasing SO2 tolerance with increasing hydrophobicity of the thallus surface in a set of 50 lichen species suggests that SO2 tolerance in lichens primarily depends on the ability to reduce uptake of acidic precipitation containing SO2 and its derivatives formed in aqueous solution.” Contact angles of water droplets placed on the surface of lichens of different SO2 tolerance (A) or growth form (B). Hauck M et al. Ann Bot 2008;101:531-539 © The Author 2007. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org Strengths & Weaknesses Strengths Data significance, clarity Discusses buffering capacity of substratum Discusses characteristics of hydrophilic lichen Weaknesses Do not discuss role of systematic position, thallus morphology, or photobiont choice Do not discuss hydrophobic substances on thallus Sensitivities of some species still lacking