บทบาท หน้าที่ ห้องปฏิบตั กิ าร
ในการสนับสนุนการส่งออกสินค้าเกษตรและอาหารของกรมปศุสตั ว์
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
1
Welcome
to
Bureau of Quality Control of
Livestock Products (BQCLP),
Department of Livestock Development
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
2
สำนักตรวจสอบคุณภำพสินค้ำปศุสต
ั ว์
สำนักตรวจสอบคุณภำพสิ นค้ ำปศุสัตว์ กรมปศุสัตว์
ในกำรสนับสนุนด้ ำนควำมปลอดภัยอำหำร
บทบำท หน้ ำที่
ห้ องปฏิบัตกิ ำรอ้ ำงอิงตรวจสอบอำหำรของอำเซียนด้ ำนสำร
ตกค้ ำงยำสั ตว์ ASEAN Food Reference Laboratory
for Veterinary Drug Residue (AFRL for VDR)
บทบำทหน้ ำที่
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
3
Bureau of Quality Control of Livestock Products (BQCLP)
BQCLP Building 1
BQCLP Building 2
91 Moo 4 Tiwanon Rd., Bangkadi sub-district, Muang district,
Pathumthani province, 12000 Thailand
Tel. +66-2-967-9700 Fax. +66-2-967-9747
E-mail address: qcontrol@dld.go.th
http://dld.go.th/qcontrol
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
4
300,000,000.00
100,000,000.00
50,000,000.00
Contracted
employee,
303
121,646,400
150,000,000.00
2011
2012
31,250,100
Permanent
employee, 4
200,000,000.00
25,463,900
1,254,800
Paraveterinary
official, 1
Scientist, 25
85,769,110
77,015,440
94,084,280
92,617,600
Director, 1
Administrator, Veterinary
official, 13
6
42,014,540
50,526,724
57,669,700
62,170,620
Expert, 3
Budget (baht)
250,000,000.00
153,247,550
128,796,964
Total = 356 positions
273,400,380
186,038,320
BQCLP: Personnel & Budget
2013
2014
0.00
49 official
(16 veterinary officials ,1 para-veterinary official and 25 scientists, 6
administrators )
4 permanent employee,
303 contracted employee
(อัตรำส่ วน ข้ ำรำชกำร : พนักงำนำชกำร 1 : 6)
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
5
2. บทบำท หน้ำที่
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
6
Vision วิสย
ั ทัศน์
“To be an internationally recognized testing
laboratory for safety and quality control of
livestock products”
เป็ นห้ องปฏิบัติกำรอ้ำงอิงของประเทศ ที่ได้ รับกำรยอมรับในระดับสำกล
ในด้ ำนกำรตรวจสอบคุณภำพสิ นค้ ำปศุสัตว์
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
7
Mission พันธกิจ
 Surveillance and monitoring livestock products for food safety control
 ตรวจติดตำมเฝ้ำระว ังด้ำนควำมปลอดภัยอำหำรของสินค้ำปศุสต
ั ว์
 Providing technical assistance and training on quality and safety of
livestock products
 ให้ควำมช่วยเหลือด้ำนวิชำกำร โดยจัดกำรฝึ กอบรมด้ำนคุณภำพและควำมปลอดภัย
ของสินค้ำปศุสต
ั ว์
 Performing research & development for food safety and quality testing
methods
 ทำงำนวิจัยและพัฒนำวิธีวเิ ครำะห์ ด้ำนคุณภำพและควำมปลอดภัย
 Strengthening laboratory network capacity with local service laboratory
and collaboration with other outbound laboratories to meet International
Standard
่ บริกำรในพืนที
้ ่ ให้ควำมร่วมมือกับ
 สร ้ำงเครือข่ำยเสริมศ ักยภำพห้องปฏิบต
ั ก
ิ ำรทีให้
้
่ ฒนำศ ักยภำพห้องปฏิบต
ห้องปฏิบต
ั ก
ิ ำรทังในและนอกประเทศ
เพือพั
ั ก
ิ ำรให้เป็ นที่
ยอมร ับตำมมำตรฐำนสำกล
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
8
Mission พันธกิจ
 As the National Reference Laboratories for quality control testing of
animal feed, veterinary medicinal products, hazardous substances
and livestock products ห้องปฏิบต
ั ก
ิ ำรอ้ำงอิงระดับชำติในด้ำน กำร
ตรวจสอบควบคุมคุณภำพอำหำรสัตว ์ ผลิตภัณฑ ์ยำสัตว ์ วัตถุอ ันตรำย และ
สินค้ำปศุสต
ั ว์
 As the ASEAN Food Reference Laboratory for Veterinary Drug
Residue ห้องปฏิบต
ั ก
ิ ำรอ้ำงอิงกำรวิเครำะห ์อำหำรของอำเซียนด้ำนสำรตกค้ำง
ยำสัตว ์
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
9
บทบำท หน้ำที่
 ให้ บริกำรกำรตรวจสอบคุณภำพสิ นค้ ำปศุสัตว์ ด้ ำนควำมปลอดภัยอำหำร
 กิจกรรมต่ ำง ๆ เช่ นตรวจ เฝ้ำระวังสำรตกค้ ำง กิจกรรมตรวจรับรองเพื่อส่ งออก เนื้ออนำมัย
ฯลฯ
 วิธีวเิ ครำะห์ (พัฒนำวิธี, พิสูจน์ ควำมใช้ ได้ , ขอกำรรับรอง ISO/IEC 17025:2005)
 ฝึ กอบรม ถ่ ำยทอดเทคโนโลยีกำรตรวจสอบ เพื่อสร้ ำงเสริมศักยภำพควำมสำมำรถห้ องปฏิบตั ิกำร
 กำรดำเนินกำรถ่ ำยโอนภำรกิจ
 ให้ บริกำร คำปรึกษำแนะนำ ห้ องปฏิบตั ิกำร ผู้ประกอบกำร เกษตรกร
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
10
BQCLP Organization Chart
4 Supporting units
1. General Administration
Section ฝ่ ายบริ หารงานทัวไป
่
2. Laboratory Management
Division ส่วนบริ หารงานจัดการห้องปฏิ บตั ิ การ
3. Laboratory Quality System
Division กลุ่มพัฒนำระบบคุณภำพห้ องปฏิบัติกำร
4 Laboratory divisions
1. Feed Quality Control Laboratory Div.
กลุ่มตรวจสอบคุณภาพอาหารสัตว์
3. Veterinary Public Health Laboratory Div.
กลุ่มตรวจสอบคุณภาพเนื้ อสัตว์และผลผลิ ตจากสัตว์
BQCLP
4. Laboratory Audit Division
กลุ่มรับรองห้องปฏิ บตั ิ การ
2. Veterinary Drug Assay Division
กลุ่มตรวจสอบคุณภาพยาสัตว์
4. Milk and Milk Products Quality Control
Laboratory Div. กลุ่มตรวจสอบคุณภาพนมและผลิ ตภัณฑ์นม
11
Samples for testing
Feed & feed additive
Veterinary medicinal products
Fat
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Livestock products
9 Julu 2014
12
BQCLP Testing Activities
Source of samples
Farm,
Manufacture of
animal feed,
port of entry
Farm,
Drug stores
Farm,
slaughterhouse,
processing plants,
port of entry
Dairy farm, Milk
collecting center
Test samples
Sample collectors
(Customer)
BQCLP
Feed additives and
Animal feed
Farmer,
DLD Inspector
Veterinary drugs,
Hazardous
Substances
DLD Regional officer
(Veterinarian)
Meat & animal
products
Authorized personnel/
veterinarian
LIMS
Farmer,
DLD Inspector
DLD
Traceability
database
Milk & Milk
products
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Test Reports
9 Julu 2014
13
1. Feed Quality Control Laboratory Division (FQCL)
กลุ่มตรวจสอบคุณภำพอำหำรสัตว ์
Quality and safety
• Quality Assurance System
• Monitoring Plan
Feed analysis
• Inspection and collection for
quality control
• Research on public health
implications of animal feed
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Monitoring and quality control of feed to fulfill
feed quality control acts BE 2525 and additional
BE 2542 (1982) and international standard (EU ,
FDA etc.)
Setting the standard
Method development and
method validation
Research for feed quality control
and feed safety in farm
9 Julu 2014
14
1. Feed Quality Control Laboratory Division (FQCL)
กลุ่มตรวจสอบคุณภำพอำหำรสัตว ์
Analytical method & Instrummentation
Physical inspection
Screening test
Microscope
ELISA
CHARM II
Determination
HPLC-UV
Confirmation
LC-MS
LC-MS/MS
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
15
1. Feed Quality Control Laboratory Division (FQCL)
กลุ่มตรวจสอบคุณภำพอำหำรสัตว ์
Test Samples : Raw Materials, Feed, Feed Additives, Premixes, etc.
Physical :
Drugs, Vitamins and Minerals Screening
test, Ingredient and Adulteration
Chemical :
Proximate and Nutrient analysis, Vitamins,
Minerals, Urea, Urease activity and Heavy
metal
Microbiological :
Pathogenic bacteria, Total bacteria and
Mould count, Microbiological assay,
DNA&GMOs (Real-time PCR) and
Probiotics
Toxicology and Biochemistry
Mycotoxins : Aflatoxin, Ochratoxin,
Zearalenone,
Fumonisin and T-2 Toxin,
Deoxynivalenol(DON)
Hormone, Veterinary Drug and
Pesticides, PCBs, Melamine
Dioxin, etc.
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
16
2. Veterinary Drug Assay Division (VDAD)
กลุม่ ตรวจสอบคุณภาพยาสัตว์ (และวัตถุอนั ตราย)
1. Testing samples
• Post-marketing veterinary
medicinal products
• Hazardous substance
products
• Livestock drinking water
• Antimicrobial resistant
microorganisms
2. Method development & Method validation
3. Research in related fields
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
17
2. Veterinary Drug Assay Division (VDAD)
กลุ่มตรวจสอบคุณภำพยำสัตว์ (และวัตถุอนั ตรำย)
1. Veterinary medicinal
products
• Identification
• Assay of active ingredients
colistin/gentamicin/
oxytetracycline/
streptomycin/ tylosin
amoxicillin/ enrofloxacin/
doxycycline/ lincomycin/
penicillin/ sulphonamide/
trimethoprim/ tiamulin
albendazole/ ivermectin/
chlortetracycline/
clorsulon
• pH
• Water content/Loss on
drying
[Pharmacopoeia]
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
2. Hazardous
substance products
• Assay of Active
ingredients
 Aldehyde group
 Quaternary
Ammonium
compounds
 Hypochlorite as
available chlorine
 Hydrogen peroxide
and Peracetic acid
• Efficiency testing
 Phenol coefficient/
AOAC used dilution
technique
3.Prohibited
substances
• Determination of
prohibited
substances in feed
water
 Nitrofurans
 Chloramphenicol
 Nitroimidazole
 Beta – agonist
4. Antimicrobial
resistance (AMR)
• Disk diffusion
• MIC
• PCR
9 Julu 2014
18
2. Veterinary Drug Assay Division (VDAD)
กลุ่มตรวจสอบคุณภำพยำสัตว์ (และวัตถุอนั ตรำย)
Annual surveillance of veterinary medicinal products quality
Veterinary
Drug Supplier
/ Farm
DLD,
MOAC
77 Provincial
Livestock
Offices
BQCLP
AFVC
Thai FDA /
MOPH
AFVC
= Division of Animal Feed and Veterinary Products Control กองควบคุมอาหารและยาสัตว์
BQCLP = Bureau of Quality Control of Livestock Products สานักตรวจสอบคุณภาพสิ นค้าปศุสัตว์
DLD, MOAC = Department of Livestock Development, Ministry of Agriculture and Cooperative
Thai FDA, MOPH = Food and Drug Administration, Ministry of Public Health
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
19
3. Veterinary Public Health Laboratory Division (VPHL)
กลุ่มตรวจสอบคุณภำพเนื้อสัตว์และผลผลิตจำกสัตว์
1. Livestock products food safety, quality surveillance and monitoring
testing for
•National residue control plan (NRCP)
•Microorganisms (e.g. food hygienic indicators, food borne pathogens
•Veterinary drug residues, pesticide residues, contaminants, mycotoxins
•Food authenticity (e.g. species identification for Halal food)
•Others:- dyes
2. As Reference Laboratory (National / ASEAN Food Reference Laboratory)
Information center for test method, RM/CRM, experts
Training center
Proficiency Testing Provider (PTP) / Inter-Laboratory comparison (ILC)
3. As a leading laboratory:- Research and development for high efficient and
performance test methods
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
20
3. Veterinary Public Health Laboratory Division (VPHL)
กลุ่มตรวจสอบคุณภำพเนื้อสัตว์และผลผลิตจำกสัตว์
VPHL comprises 3 sections of laboratories
1. Food Chemistry,
chemical residues
and contaminants
 Pesticide residues
analyzed by
GC-ECD, HPLC-UV,
GC-MS/MS, LC-MS/MS
 Heavy metal analyzed
by AAS, ICP-MS
 Aflatoxins by HPLCFD
 Dye e.g. Canthaxantin
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
2. Veterinary Drug and
Hormones Residues
3. Hygiene and Food
Microbiology
[ASEAN Food Reference
Laboratory for Veterinary
drug residues]
 Screening test by ELISA,
Biosensor, HPLC, etc.
 Screening and
confirmation by
LC-MS/MS,
 Multi residue screening
and confirmation by
LC-QToF
 Food hygienic
microorganism
 Food borne pathogens
 Microbiological Assay
(6-plate test)
 Porcine DNA by RTPCR
by conventional culture
method, ELISA and
molecular method
9 Julu 2014
21
4. Milk and Milk Product Quality Control Laboratory (MMPQCL)
กลุม
่ ตรวจสอบคุณภาพนมและผลิตภ ัณฑ์
Physical
• Reduction test
• pH
• Freezing point
Biological
• Standard plate count
• Lab. Pasteurization count
• Coliform
Chemical
• Milk composition (fat, protein, etc)
• Somatic cell
Screening
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
• Antibiotic screening test
• การเติ มนมผงในน้านมดิ บ -Deoxyketone
9 Julu 2014
22
4. Milk and Milk Product Quality Control Laboratory (MMPQCL)
กลุม
่ ตรวจสอบคุณภาพนมและผลิตภ ัณฑ์
Improve farm
management for
good quality and
better price
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
23
แผนและผลกำรตรวจต ัวอย่ำงต่อปี
LABORAT
ORY
VPHL
FQCL
VDAD
MQCL
2011
44,575 /
77,681
6,786 /
7,557
1,365 /
1,362
24,000 /
57,576
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Plan/Results (Samples)
2012
2013
39,755 /
53,799 /
56,671
80,358
6,902 /
8,569 /
7,111
1,654 /
1,558 / 1,199
1,496
52,000 /
52,000 /
55,193
62,997
2014
47,183 /
8,600 /
1,558 /
52,000 /
9 Julu 2014
24
กำรพัฒนำและกำรพิสูจน์ควำมใช้ได้ของวิธ ี
วิเครำะห ์
Laboratory
FQCL
Method development 2011
1. Sudan Red I analysis in animal feed by LC-MS/MS
2. Inhibitory in animal feed microbiological assay sensitivity test of 5 reference
bacteria with 10 antibiotics
3. Coccidiostat group : Nicarbazin analysis in animal feed by LC-MS/MS
Laboratory
FQCL
Method validation 2011
1. Deoxynivalenol analysis in chicken feed by LC-MS
2. Aflatoxins analysis in corn by Immunoaffinity column / Fluorometry
3. Nitroimidazole analysis in animal feed by LC-MS/MS
4. Organochlorinated pesticides and polychlorinated l biphenyls analysis in
animal feed by GC-MS/MS
5. Crude fiber analysis in chicken feed
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
25
กำรพัฒนำและกำรพิสูจน์ควำมใช้ได้ของวิธ ี
วิเครำะห ์
Laboratory
FQCL
Method validation 2011 (cont)
6. Aflatoxins analysis in chicken feed with IAC – post column
photochemical reaction by HPLC – Fluorescence detection AFB1,
AFB2, AFG1, AFG2
7. Cadmium analysis in corn by ICP-OES
8. Adulterant in corn meal by microscopic analysis
9. Adulterant in soybean meal by microscopic analysis
10. Tetracyclines analysis in animal feed by HPLC: oxytetracycline,
chlortetracycline, doxycycline
11. Total mold count by ISO21527 – 2 : 2008 for animal feed ; Total
mold count from < 10 to 10n cfu/g
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
26
Laboratory research and development
Method development 2012
Laboratory
FQCL
• Development for the detection enumeration of
Enterobacteriaceae in animal feed
• Development and validation of detection protein content in
corn, soybean and fish meal by NIRS
• Validation of aflatoxins analysis in cattle feed with IAC-post
column photochemical reaction by HPLC-fluorescence detection
• Validation of aflatoxins analysis in chicken feed by
immunoaffinity column / fluorometry
• Method validation for determination of cadmium in poultry
feed by ICP-OES
• Method validation for determination of carbadox in swine feed
by LC-MS/MS
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
27
Laboratory research and development
Method development 2013
Laboratory
FQCL
•
Application of Near Infrared Spectroscopy for determination of nutrients
content in fish meal adulterated with feather meal
•
Development of the dioxins method in animal feed using high resolution GCMS
•
Method validation for determination of nicarbazin, salinomycin and narasin in
layer chickens feed by LC-MS/MS
•
Validation of aflatoxins analysis in swine feed with IAC-post column
photochemical reaction by HPLC-fluorescence detection
•
Validation of zearalenone analysis in corn by immuno-affinity column /
fluorometry
•
Validation of aflatoxins analysis in cattle feed by immuno-affinity column /
fluorometry
•
Method validation for determination of cadmium in soy meal (wet digestion) by
ICP-OES
•
Validation of enumeration of Enterobacteriaceae in animal feed (ISO 21528-2:
2004)
•
Method validation for determination of enrofloxacin in chicken feed by LCMS/MS
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
28
กำรพัฒนำและกำรพิสูจน์ควำมใช้ได้ของวิธ ี
วิเครำะห ์
Method development & validation 2011
Laboratory
VDAD
2011
VDAD
2012
VDAD
2013
•
Method development and validation of penicillin G and streptomycin assay in veterinary medicinal
products
•
Method development and validation of coumatetralyl assay in hazardous substance products for
livestock
•
Method development and validation for trace analysis of fluoroquinolones in livestock drinking water
using LC-MS ion trap
•
Method development and validation for trace analysis of Beta-agonists in livestock drinking water
using LC-MS ion trap
•
Method development and laboratory set-up procedure for testing of hazardous substance products
•
Method validation for amitraz determination in hazardous substance products using
HPLC-DAD
•
Method development for active ingredient assay of hazardous substance products
•
Method development and laboratory set-up procedure for testing of hazardous substance
products
•
Method development for phenolic compounds determination in hazardous substances
products for livestock using HPLC-DAD
•
Method validation for amitraz determination in hazardous substance products for livestock
using HPLC-DAD
•
Method development and capacity building of hazardous substance products for livestock
Sujittra Phongvivat
BQCLP
DLD @
testing
laboratory
Maruay Hotel
9 Julu 2014
29
กำรพัฒนำและกำรพิสูจน์ควำมใช้ได้ของวิธ ี
วิเครำะห ์
Laboratory
Method development & validation 2011
VPHL
•
Determination of mercury and lead contaminant in animal tissue and liver
2011
•
Determination of Aminoglycosides in honey by LC-MS/MS
•
Study on application of real-time ultrasound for screening Beta-agonist abused in
finishing pigs
•
Development method for the determination of 16 PAHs in sausage by GC-MS/MS
•
Method development of spinosad in animal fat analysis by HPLC-UVD
•
Method development and validation for confirmation of aminoglycosides in pig
muscle by LC-MS/MS
•
Method development for confirmation of tetracyclines residues in honey by LCMS/MS
•
Development and method validation for determination of meat contaminations using
Real-time PCR
•
Validation for confirmatory method for tetracyclines residues in honey by LC-MS/MS
VPHL
2012
VPHL
2013
• Method
for confirmation of amitraz residues in honey by GC-MS/MS
Sujittra Phongvivat
BQCLPdevelopment
DLD @
9 Julu 2014 30
Maruay Hotel
Laboratory quality system development division
กลุ่มพัฒนำระบบห้องปฏิบต
ั ก
ิ ำร
1. Laboratory Quality System Section
พัฒนำระบบคุณภำพห้ องปฏิบัตกิ ำรของ สตส
ให้ เป็ นไปตำมข้ อกำหนดทั่วไปว่ำด้ วยควำมสำมำรถของห้ องปฏิบัตกิ ำรทดสอบและห้ องปฏิบัตกิ ำรสอบเทียบ
(ISO/IEC 17025:2005)
2. In-house Calibration Laboratory
ให้ บริกำรกำรสอบเทียบ เครื่ องแก้ว เครื่ องชั่ง ที่ใช้ ใน สตส
(ISO/IEC 17025:2005 accredited)
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
31
Laboratory Accreditation (ISO/IEC 17025:2005)
Laboratory section
Testing scope
Food Chemistry, Residues and Contaminants
7
Veterinary Drug and Hormone Residues
6
Food Hygiene and Microbiology
15
Veterinary Drugs and Hazardous Substances
Assay
5
Feed Quality Control
15
Calibration
2
Total
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
50
9 Julu 2014
32
ISO/IEC 17025:2005 Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Laboratory Section
Food Chemistry,
Residues and
Contaminants
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Type of Sample
-
Total Arsenic
Cadmium
Lead
Total Mercury
Liver, Muscle
Honey
-
Organochlorine Pesticides
(OCPs)
Animal fat
9 Julu 2014
33
ISO/IEC 17025:2005 Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Laboratory Section
Veterinary Drug and
Hormone Residues
Type of
Sample
-
Sulfonamides
-
Protein-bound
Nitrofurans Metabolites
- Animal tissue
and processed
animal tissue
- Egg
- Honey
-
Beta-agonist
Brombuterol, Cimaterol,
Cimbuterol, Clenbuterol,
Hydroxymethylclenbuterol,
Mabuterol, Mapenterol,
Ractopamine, Salbutamol and
Zilpaterol
- Urine
- Muscle
- Liver
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Muscle
9 Julu 2014
34
ISO/IEC 17025:2005 Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Laboratory Section
Veterinary Drug and
Hormone Residues
-
Chloramphenicol
-
Fluoroquinolones;
Enrofoxacin and Ciprofloxacin
-
Tetracyclines;
Oxytetracycline,
Tetracycline,
Chlortetracycline
Doxycycline
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Type of
Sample
-
Animal tissue
Egg
Honey
Milk
Muscle
Egg
- Muscle
9 Julu 2014
35
Laboratory Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Laboratory Section
Food Hygiene and
Microbiology
Type of Sample
Anti-bacterial substances residues Meat and Meat
Clostridium perfringens
Products
Escherichia coli and Coliform
Enterococcus
Enterococcus (in water and ice)
Enterobacteriaceae
Listeria monocytogenase
Salmonella spp.
Staphylococcus aureus (BPK)
Staphylococcus aureus (RPF)
Staphylococal enterotoxin
Total bacterial count
Yeast & Mould
Presumptive Determination of
Escherichia coli (Water and Ice)
- @Determination of Salmonella spp.
Sujittra Phongvivat BQCLP DLD
Maruay Hotel
-
9 Julu 2014
36
Laboratory Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Laboratory Section
Feed quality control
-
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Aflatoxins in corn
Total Aflatoxins in corn
Adulteration feather meal in
fish meal
Adulteration in soybean by
microscope
Adulteration in corn by
microscope
Determination of Cadmium in
soybean meal
Determination of Cadmium in
corn by ICP-OES
Type of Sample
Raw material and
animal feed
9 Julu 2014
37
ISO/IEC 17025:2005 Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Laboratory Section
Feed quality control
-
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Antibiotic inhibitory
microbiological assay
Beta-agonist in complete feed
for swine
Fiber in poultry feed
Protein in poultry feed
Salmonella spp. in feed and
animal feeding stuffs
Tetracycline, Oxytetracycline
Chlotetracycline, Doxycycline
in poultry feed
Total plate count in feed and
animal feeding stuffs
Type of Sample
Raw material and
animal feed
9 Julu 2014
38
ISO/IEC 17025:2005 Accreditation
Testing Scope
(ISO/IEC 17025 Accredited)
Type of Sample
-
Quality assay of enrofloxacin
drug using HPLC
Determination of Beta-agonist
drug in livestock water using
LC-MS iontrap
Veterinary
medicinal
products
Livestock
drinking water
-
Tylosin
Injectable
Solution
-
pH
Veterinary
medicinal
-
Use dilution
Laboratory Section
Veterinary Drugs and
Hazardous Substances
Assay
-
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
Disinfectant
9 Julu 2014
39
กลุ่มรับรองห้ องปฏิบตั ิกำร
กำรดำเนินกำรถ่ ำยโอนภำรกิจ ตำมมติครม 11 พ.ค. 2553
ห้องปฏิบตั ิ การเอกชนที่ผา่ นเกณฑ์การประเมินและได้รบั มอบการถ่ายโอนภารกิจแล้ว
จานวน 47 ห้องปฏิบตั ิ การ
 ปี พ.ศ. 2554 จานวน 26 ห้องปฏิบตั ิ การ
 ปี พ.ศ. 2555 จำนวน 12 ห้ องปฏิบตั ิกำร
 ปี พ.ศ. 2556 จำนวน 9 ห้ องปฏิบตั ิกำร
Total 47 laboratories
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
40
International Activities
ASEAN Food Reference Laboratory for Veterinary Drug
Residues Since 2004
• AFRLs network was initiated at
the Workshop and Expert
Consultation on “Strategies for
Strengthening Food Testing in
ASEAN”
In Jakarta, Indonesia on 7 – 11
June 2004 under the EC- ASEAN
Economic cooperation
Programme on Standards, Quality
and Conformity Assessment.
•Veterinary Public Health Lab was
selected and assigned as AFRL for
Veterinary Drug Residues since
then.
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
43
International Activities
AFRL for VDR activities
 Provide training & technical
advice & services to ASEAN
Member Countries (AMCs)
 Become resource &
information center i.e. test
method for vet drug residues,
experts, Certified Reference
Materials/ Reference Materials
(CRM/RM)
 Coordinate inter-laboratory
comparisons or proficiency
testing scheme for veterinary
drug residues
 Strengthening laboratory
cooperation network for
consumer protection
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
44
International Activities
BQCLP :- VDAD – AHRI (Taiwan)
Building Capacity of Veterinary Drugs Testing Laboratory
between Thailand and Taiwan (R.O.C) Since 2011 (study visit)
9 - 15 May 2011
2 person
18 - 22 September 2012
3 person
22 - 26 July 2013
2 person
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
45
BQCLP staff participation in international committee
CODEX Committee of Food Contaminants
CODEX Committee of Food Hygiene (CCFH)
CODEX Committee on Residue of Veterinary
Drugs in Food (CCRVDF)
CODEX Committee on Method of Analysis
and Sampling (CCMAS)
CCFH Physical Working Group on Revision of the
Principles for the Establishment and Application of
Microbiological Criteria for Foods
ISO Technical Committee for Microbiology :
ISO/TC 34/SC9 Meeting
ISO Technical Committee for Horizontal
Meeting
Methods34/SC9
for Molecular
Biomarker Analysis :
ISO/TC
34/SC16
Meeting
Sujittra Phongvivat
BQCLP
DLD @
Maruay Hotel
9 Julu 2014
46
BQCLP as a Learning Organization
BQCLP as a learning organization (LO)
through knowledge management (KM):BQCLP encourages our staff to continually
self studying and to improve their working
performances:By - Internet accessible
- Library
- Internal broadcasting
- In house training
- Attending seminar, conference &
workshop by close collaboration/
communication through
- Coffee time open discussion
- Knowledge/best practice sharing
through
- Community of Practitioner (CoP), KM
forum by production of work
manual/SOP to be followed for highest
quality of work.
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
47
4. Laboratory plan:
2014-2018
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
48
1. Extending scope of accredited method
Plan / Year
FQCL
1. Testing method in animal feed
VDAD
1. Testing method in VD & HS & feed water
VPHL
1. Pesticide residues in animal fat
2. Nitrofuran metabolites residue (Total residues) in egg
and honey using LC-MS/MS
3. Sulfonamides residue in honey using LC-MS/MS
4. Avermectin using HPLC-FD
5. Campylobacter in food
6. Testing method for veterinary drug residues in meat
and meat product
MMPQC
1. Aerobic plate count in milk (raw and processed)
2. Coliform in milk (raw and processed)
3. Testing method in milk (raw and processed)
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
2014
2015
2016
2017
2018
3 scopes 3 scopes
2 scopes
3 scopes
2 scopes
1 scope
1 scope
1 scope
1 scope
1 scope 1 scope
1 scope
1 scope
3 scopes
9 Julu 2014
49
2. Method development and validation
Plan / Year
FQCL
1. Dioxin in meat and bone meal
2. GMOs in meat and bone meal
3. Species identification in meat and bone meal
4. Color additive in animal feed
5. Testing method for heavy metals and veterinary drugs
in animal feed
6. Molecular method development for DNA base
sequencing using MALDI-TOF MS
7. Testing method development for heavy metals and
mineral using ICP-MS
VDAD
1. Veterinary drugs & Hazardous substance products
2. Hazardous substances laboratory development
VPHL
1. Arsenic speciation analysis in food of animal origin by
ICP-MS
2. Veterinary drug residue by LC-MS/MS and
Sujittra Phongvivat BQCLP DLD @
LC-QToF
Maruay Hotel
2014
2015
2016
2017
2018
/ (MD)
/ (MD)
/ (MD)
/ (MD)
/ (MD)
/ (MD)
/ (MD)
2 (MD/MV) 2 (MD/MV) 2 (MD/MV) 2 (MD/MV) 2 (MD/MV)
4 items 4 items 4 items 4 items 4 items
1 (MD)
1 (MV)
1 (MD/MV) 1 (MD/MV)
9 Julu 2014
50
3. Laboratory facilities development
Plan / Year
2014
2015
2016
2017
2018
FQCL
1. Bio-molecular laboratory for animal feed testing
/
2. Rapid alert and monitoring system development for animal feed
safety
3. Sample preparation for inter-laboratory comparison of
contamination of veterinary drugs, mycotoxins and heavy metals
in animal feed
4. Reference animal feed quality testing laboratory (i.e. adulteration,
mycotoxins, nutrition enrichment (protein, fat, fiber, moisture,
etc.))
5. Training center for analysis of contaminant in animal feed and
corrective action for feed quality control of Thailand
VDAD
1. Monitoring and surveillance project for antimicrobial resistant
microorganism in livestock products
VPHL
1. PT scheme for veterinary drug residues (round/year)
2. Microbiological assay interlaboratory comparison (round/year)
MMPQC
1. Ring test for milk and milk products quality control laboratory
(round/year)
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
/
/
/
/
/ (pilot)
/
/
/
/
1
1
1
1
2
1
2
1
2
1
4
4
4
4
4
9 Julu 2014
51
4. Training course
Plan / Year
VDAD
1. Pharmaceutical analysis workshop
2. Antimicrobial resistant (AMR) workshop
VPHL
1. Hygiene and food microbiological training course
2. Veterinary drug residue analysis/ AFRL veterinary drug
residue workshop
MMPQC
1. Milk and milk products quality testing course
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
2014
2015
2016
2017
2018
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
9 Julu 2014
52
5. Capacity building project & Testing service
Plan / Year
VDAD
1. Capacity Development of Veterinary Drugs Testing
Laboratory between Thailand and Taiwan (R.O.C)
2. Capacity Building for Testing of Hazardous Substances in
Livestock Production Testing Laboratory under TCPF with
FAO
3. Plan to propose for twinning laboratory project (Veterinary
medicinal products) with OIE Collaborating Center
(France)
4. Project Proposal Application for Enhancing National
Capacities for Antimicrobial Resistance Risk
Management in Food Animal Production in Thailand with
FAO (ongoing)
Plan / Year
2014
2015
2016
2017
2018
2016
2017
2018
/
/
/
/
/
2014
2015
VDAD
Efficiency of disinfectant by Use-dilution
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
/
9 Julu 2014
53
Thank you
Sujittra Phongvivat BQCLP DLD @
Maruay Hotel
9 Julu 2014
54