pineapple enzyme lab

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Digestive System &
Enzymes
1/29/2016
•
SWBATDiscover form(s) and function(s)
of the digestive system along with
purpose of Enzymes
*Students will begin phase one of
enzyme lab.
• In:
-DNA-Gel Electrophoresis Viewing
-Digestive Parts
-Bozeman Video
• Through:
-Notes on Digestive System
-Set Up Enzyme Lab
OUT:
-HW: Finish Digestive notes from online
Calendar
Pineapple enzymes
• BACKGROUND: The enzymes in some tropical
fruits can break down protein. For example
meat tenderizers have the enzyme, papain,
that breaks down some of the fiber. We use it
to soften up a tough piece of meat. The
papain comes from the papaya fruit.
Pineapple has a digestive enzyme called
bromelin.
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2.
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8.
PROCEDURE:
With the razor blade carefully cut equal sized pieces of fresh and canned
pineapple.
The pieces should be just small enough to slide into your cups.
Label the cups.
Pour 25 ml of liquid gelatin into each of the cups.
Place the different pieces of pineapple into the first 2 cups.
See the table below to match up the contents. Notice that cup #3
contains only gelatin.
Mix the contents of the cups by rolling them upright between the palms
of your hands.
Place cups into the refrigerator.
CUP CONTENTS:
1. Gelatin and Fresh Pineapple
2. Gelatin and Canned Pineapple
3. Gelatin (Mr. Loew’s)
Pineapple enzyme lab
(on your own paper)
Purpose• What is the question you are trying to
answer?
Hypothesis• Predict what will happen to the jello.
Observations and analysis
• Observations:
– Create a table and record 2 observations for each cup.
– Take 2 observations. One observation today, second observation
tomorrow.
• Analysis Questions:
1. Why did cup #3 (Mr. Loew’s) contain only gelatin?
2. What could account for the different results in the
cups?
3. How could the canning process change the enzyme
bromelin?
4. Why is it important for pineapple factory workers to
wear masks?
5.Design an experiment to find a temperature at which
an enzyme works the best? Identify the constants, IV,
DV, and control.
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