Unit 3.4 Populations and environment 3.4.2: What is ATP? Keywords Adenosine Triphosphate, Adenosine Diphosphate, Mitochondria, Electrochemical Gradient, ATP Synthase, Energy Recap Write the word and symbol equations for photosynthesis and aerobic respiration. Draw and label a diagram of a mitochondrion. carbon + water dioxide light energy oxygen + glucose light energy 6CO2 + 6H2O oxygen + glucose 6O2 + C6H12O6 6O2 + C6H12O6 carbon + water + energy dioxide 6CO2 + 6H2O Mitochondria The mitochondrion is an energy-generating organelle. It is surrounded by two membranes. The inner layer folds inwards to form the cristae. The cristae project into a liquid called the matrix. outer membrane cristae matrix inner membrane The inner membrane is coated in enzymes, which catalyze the reactions of aerobic respiration to produce ATP. 5 of 37 © Boardworks Ltd 2008 Y13: What Y13 we know so far… What we know so far… 1. The structure of mitochondria 2. All living cells respire 3. Respiration is the release of energy in a series of controlled reactions. 4. One of the products of respiration is adenosine triphosphate (ATP) Y13: Learning objective All Pupils will : 1. Recall the structure and function of a mitochondria 2. Learn the structure and synthesis of adenosine triphosphate (ATP) and adenosine diphosphate (ADP) 3. Learn the role of ATP as the immediate source of energy for biological processes. Y13: Learning outcome All Pupils will be able to: 1. 2. 3. Draw and label a diagram of ATP Write a word equation for the conversion of ADP to ATP List four biological processes where ATP synthesis is required as an immediate source of energy Most Pupils will be able to: 1. Predict the word equation for the conversion of ATP to ADP Some Pupils will be able to: 1. Hypothesize the consequences of an ATP deficiency Y13 What is ATP? ATP STRUCTURE Y13 ATP stands for: Adenosine Triphosphate What is ATP? ATP STRUCTURE Y13 What is ATP? ATP stands for: Adenosine Triphosphate It is made up of: 1. Adenine (Base) 2. Ribose (Sugar) 3. Three inorganic phosphates (3Pi) ATP STRUCTURE Y13 What is ATP? ATP stands for: Adenosine Triphosphate It is made up of: 1. Adenine (Base) 2. Ribose (Sugar) 3. Three inorganic phosphates (3Pi) ATP STRUCTURE What is ATP? Y13 ATP stands for: Adenosine Triphosphate ATP STRUCTURE It is made up of: 1. Adenine (Base) 2. Ribose (Sugar) 3. Three inorganic phosphates (3Pi) ATP is therefore a phosphorylated nucleotide Y13 ATP synthesis So ATP is a nucleotide, but it also has another function as an: ENERGY ‘STORAGE’ MOLECULE. ATP is built up from the molecules adenosine diphosphate (ADP) and ______________. ATP ADP Y13 ATP synthesis So ATP is a nucleotide, but it also has another function as an: ENERGY STORAGE MOLECULE. ATP is built up from the molecule: adenosine diphosphate (ADP) and inorganic phosphate (Pi). ATP ADP What is ATP? Y13 To synthesise ATP from ADP, Pi and ______ is required. Pi ADP Pi Pi ATP Pi Pi Pi Y13 Whiteboards So, to convert ADP to ATP we need inorganic phosphate, and something else. What do you think this is? What is ATP? Y13 To synthesise ATP from ADP, Pi and energy is required. Energy Pi ADP Pi Pi ATP Pi Pi Pi Y13 What is ATP? ADP + Pi + Energy ATP (+ Water) The addition of phosphate to ADP is termed phosphorylation. There are different types of phosphorylation: photo, oxidative and substrate-level. We will learn more about these through the topic. Y13 Whiteboards Write down a word equation for the conversion of ATP to ADP. Y13 Hydrolysis of ATP ATP (+ Water) ADP + Pi + Energy The hydrolysis of ATP to ADP is catalysed by the enzyme ATP synthase, releasing 30.7kJ mol-1 of energy. Y13 Brainstorm ATP Role of ATP Y13 Muscle Contraction: Metabolic processes: ATP Activation of molecules: Active Transport: Y13 Role of ATP Metabolic processes: ATP provides energy needed to build up macromolecules from basic units. Muscle Contraction – provides energy to allow muscle filaments to slide past each other. ATP Activation of molecules: when a phosphate is transferred from ATP to another molecule it makes it more reactive, lowering its activation energy. Active Transport: provides energy to change shape of carrier proteins in plasma Membranes. To Do: Complete Worksheet (both sides) Peer Mark Final question to be completed for homework due next lesson. Y13 Fin. THE END Y13 To Finish… To reach the top of the cliff, describe 6 keywords you’ve learnt today about ATP and energy......... http://www.teachers-direct.co.uk/resources/quiz-busters/quiz-busters-game.aspx?game_id=63908 ATP synthesis Y13 ATP synthesis takes place on the mitochondrial inner membrane. ATP synthesis requires energy. But where does this energy come from? ATP SYNTHASE Y13 ATP synthase ATP synthase is a large complex of membrane bound protein. There is a difference in charge between the mitochondrial matrix and intermembrane space. There is a difference in the concentration of Hydrogen ions (H+) between the mitochondrial matrix and intermembrane space. Hydrogen ions (or protons) flow from the intermembrane space, down this electrochemical gradient, and through the ATP synthase complex, into the matrix Electrochemical Gradient: a gradient that involves both CHARGE and CONCENTRATION ATP synthesis Y13 Outer Membrane Intermembrane Space H+ H+ H+ ATP synthase H+ Matrix 2. This prompts the release of a H+ from ATP synthase in to the matrix H+ Energy ADP Inner Membrane 1. H+ enters the ATP synthase complex from the inter membrane space H+ ATP 3. This transfer of H+ down a gradient generates enough energy at ATP synthase to produce ATP from ADP and inorganic Phosphate (Pi).