From the Box to
Packing in 30 minutes
Pack-in-a-Box
components
• Remove components and assemble
bracket as shown
• Follow the instructions to pack your
first column and learn how to use the
system
Additional Equipment &
Chemicals
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¼” wrench
5/8” wrench
7/16” wrench
Phillips head screwdriver
30ml beaker
Waste container
Methanol
Chloroform
Spatula
Balance
Computer
Ultrasonic bath
Column Packer
Assembly
• Pre assembled for supplied SSI
column hardware.
Mounting Packing Stand
to Pump
• Screw stand to side of pump
using the two #6 screws
provided.
Plumbing the Pump
Priming pumps
Use MeOH for this
column
• Open prime purge
valve press prime
button
• With prime purge
valve open use the
supplied syringe to
draw solvent
through the pump
• Prime pump
• When pump is
primed close the
prime purge valve
Packing Ring Seal
Installation
• Place ring in adapter as shown
• Make sure ring is flat in bottom
of adapter
Packer Mounted to
Stand
• Mount packer
to stand and
tighten wing
nuts
• No wrenches
are required to
seal reservoir
• Hand tighten
end nuts
Column Assembly
• Assemble lower fitting of
column and add frit to top end
fitting and set aside to put on
column after it is packed.
System Assembled
Correctly
• The use of the Quick-Set
software is not required but
gives the most flexibility and
will be used for this example
Installing Quick Set
• Insert Disk
• Run the Quick-Set “setup.exe”
program file & follow prompts.
• Due to the many versions of
Windows in use, your PC may have
newer files than the ones being
loaded. You will be prompted for a
course of action, when in doubt,
“keep” your existing files or
“ignore” other messages.
• Connect RS 232 Cable provided to
the computer and pump
• Turn on pump
Click QuickSet
• Go to Start/Programs
• Click on Quick-Set
• The screen below appears with
the information on the program
Automatic Set Up
 Initial screen is for
locating the SSI /
LA pump
 If communication
does not initialize
or if the pump is
not on this list:
Click on the “Poll
Com Port For
Device” button.
 This will scan 9
possible PC COM
ports for the
attached device.
 When the device is
found, click on
the” Continue”
button.
Getting To CP Screen
Setting Flow and
Pressure
• Set to pressure limited ramp and click
‘send’
• Set upper pressure to 9999 psi; click ‘send’
• Set lower pressure to 0 psi; click ‘send’
• Set ramp speed to 100; click ‘send’
• Set max flow and pressure from table
Adding Steps
Packing program to load
Pressure
Flow Rate
Time
9000
24
1
9000
23
5
9000
22
30
Send to pump
Saving Your Packing
Program
• Click on “Save Settings”
• Enter file name
• Save
Mounting Column
• Make sure
packing ring
seal is in
adapter
• Hand tighten
column in
adapter
• Place plug in
bottom of
column
• Remove plug
just prior to
starting pump
Tighten Column on
Packer
• Use a ¼”
wrench and
5/8” or
adjustable
wrench to snug
column to
packer
• Do not over
tighten ¼ turn
past snug is fine
• Fill column
with 3 mL
MeOH
Setting up Graphing and
Data Collection
Saving Packing Data
• Select Log data
• Give file a name
Making the Slurry
• Weigh 3 gm of silica into 30 mL
beaker
• Add 20 ml of 50:50
Chloroform:Methanol (Straight
MeOH can be used but is not as
efficient)
• Use a spatula to break up packing
• Place in ultrasonic bath until
uniformly dispersed
• Pour in packing reservoir as soon as
possible and pack column
Adding Slurry
• Use 20 mL of
silica slurry and
pour carefully
into reservoir
• Fill reservoir
with washings
of beaker and
close
Is It Closed?
• Make sure the
reservoir is full
with slurry
• Remove silica
from threads
• Hand tighten
top nut
Make Sure You Are
Ready
• Is the program loaded?
• Is the pump primed?
• Is there a waste collection
vessel below the column?
• Did you tighten the fittings?
• Is there slurry in the reservoir
and is the reservoir closed?
• Are you set to graph the process
and save your data?
• If so, proceed
Packing The Column
• To run program, click on
“Pump;” or if program is loaded
click on “Start”
Initial Packing Profile
Running a Column
Packing Profile
Removing the Column
• Stop the pump and let the
pressure drop to 0 psi
• Carefully unscrew the column,
and using a flat spatula or razor
blade, remove excess silica
• Remove silica from threads if
present
• Place end fitting and frit on
column and seal
Removing Excess Silica
• Work quickly with a spatula
edge or razor blade
Getting Ready to Pack
Again
• Pump remaining silica from
precolumn for reuse or disposal
• Empty reservoir
• Install next column and repeat
steps