Chapter 8 The Muscular System Copyright 2010, John Wiley & Sons, Inc. End of Chapter 8 Copyright 2010 John Wiley & Sons, Inc. All rights reserved. Reproduction or translation of this work beyond that permitted in section 117 of the 1976 United States Copyright Act without express permission of the copyright owner is unlawful. Request for further information should be addressed to the Permission Department, John Wiley & Sons, Inc. The purchaser may make back-up copies for his/her own use only and not for distribution or resale. The Publishers assumes no responsibility for errors, omissions, or damages caused by the use of theses programs or from the use of the information herein. Copyright 2010, John Wiley & Sons, Inc. End of Chapter 8 Copyright 2010 John Wiley & Sons, Inc. All rights reserved. Reproduction or translation of this work beyond that permitted in section 117 of the 1976 United States Copyright Act without express permission of the copyright owner is unlawful. Request for further information should be addressed to the Permission Department, John Wiley & Sons, Inc. The purchaser may make back-up copies for his/her own use only and not for distribution or resale. The Publishers assumes no responsibility for errors, omissions, or damages caused by the use of theses programs or from the use of the information herein. Copyright 2010, John Wiley & Sons, Inc. Types of Muscle and Function Skeletal - 40–50% of total body weight- voluntary Cardiac - only in heart - involuntary Mostly movement of bone & body parts Stabilizing body positions Heart only Develops pressure for arterial blood flow Smooth- grouped in walls of hollow organs Sphincters regulate flow in tubes Maintain diameter of tubes Move material in GI tract and reproductive organs Copyright 2010, John Wiley & Sons, Inc. Muscle Functions Produce body movements Stabilize body positions Store and move substances Produce heat Copyright 2010, John Wiley & Sons, Inc. Skeletal Muscle Tissue Muscle includes: muscle fibers, connective tissue, nerves & blood vessels Wrapped in epimysium Perimysium surrounds fiber bundles called fascicles Endomysium surrounds each individual fiber Copyright 2010, John Wiley & Sons, Inc. Skeletal Muscle Tissue Well-supplied with blood vessels and nerves Terminal of a neuron on each muscle fiber Copyright 2010, John Wiley & Sons, Inc. Skeletal Muscle Tissue Copyright 2010, John Wiley & Sons, Inc. Muscle Histology Elongated cylindrical cells = muscle fibers Plasma membrane = sarcolemma Transverse (T) tubules tunnel from surface to center of each fiber Multiple nuclei lie near surface of cell Cytoplasm = sarcoplasm Copyright 2010, John Wiley & Sons, Inc. Muscle Histology Copyright 2010, John Wiley & Sons, Inc. Muscle Histology Throughout sarcoplasm is sarcoplasmic reticulum Sarcoplasm contains myoglobin Stores calcium ions Red pigmented protein related to Hemoglobin that carries oxygen Along entire length are myofibrils Myofibrils made of protein filaments Come in thick and thin filaments Copyright 2010, John Wiley & Sons, Inc. Sarcomere Copyright 2010, John Wiley & Sons, Inc. Sarcomere Filaments overlap in repeating patterns Unit structure is called sarcomere Separated by Z discs Darker area = A band associated with thick filaments H zone has no thin filaments I band has thin filaments no thick filaments Copyright 2010, John Wiley & Sons, Inc. Sarcomere Copyright 2010, John Wiley & Sons, Inc. Sarcomere Copyright 2010, John Wiley & Sons, Inc. Sarcomere Copyright 2010, John Wiley & Sons, Inc. Functional Structure Thick filament (myosin) has moveable heads (like “heads” of golf clubs) Thin filaments (actin) are anchored to Z discs Contain myosin binding sites for myosin head Also contain tropomyosin & troponin Tropomyosin blocks myosin binding site when muscle is at rest Copyright 2010, John Wiley & Sons, Inc. Sliding Filament Mechanism During contraction myosin heads bind actin sites Myosins pull and slide actin molecules (and Z discs) toward H zone I bands and H zones become more narrow Sliding generates force and shortens sarcomeres and thus fibers. Copyright 2010, John Wiley & Sons, Inc. Axon collateral of somatic motor neuron Axon terminal Nerve impulse Synaptic vesicle containing acetylcholine (ACh) Synaptic end bulb Sarcolemma Axon terminal Synaptic end bulb Motor end plate Neuromuscular junction (NMJ) Synaptic cleft (space) Sarcolemma Myofibril (b) Enlarged view of the neuromuscular junction (a) Neuromuscular junction 1 1ACh is released from synaptic vesicle Synaptic end bulb Synaptic cleft (space) 2 2 ACh binds to Ach receptor Motor end plate Na+ Junctional fold 3 Muscle action potential is produced Copyright 2010, John Wiley & Sons, Inc. (c) Binding of acetylcholine to ACh receptors in the motor end plate Neuromuscular Interaction Nerve signal triggers muscle action potential Delivered by motor neuron One neuron can trigger 1 or more fibers at the same time Neuron plus triggered fibers = motor unit Copyright 2010, John Wiley & Sons, Inc. Neuromuscular Junction Neuronal ending to muscle fiber = neuromuscular junction (NMJ) Synaptic end bulbs (at neuron terminal) Release neurotransmitter Muscular area = Motor end plate Between is synaptic cleft Copyright 2010, John Wiley & Sons, Inc. Neuromuscular Junction Copyright 2010, John Wiley & Sons, Inc. Action at NMJ 1. Release of acetylcholine (ACh) Diffuses across cleft 2. Activation of ACh receptors 3. Generation of Muscle Action Potential Repeats with each neuronal action potential 4. Breakdown of ACh Copyright 2010, John Wiley & Sons, Inc. Contraction Trigger Muscle action potential → Ca2+ release from Sacroplasmic Reticulum (SR) Ca2+ binds to troponin → Moves tropomyosin off actin sites → Myosin binds & starts cycle Copyright 2010, John Wiley & Sons, Inc. Neuromuscular Junctions Interactions Animations Neuromuscular Junctions You must be connected to the internet to run this animation. Copyright 2010, John Wiley & Sons, Inc. Contraction Cycle Myosin binds to actin & releases phosphate group (forming crossbridges) Crossbridge swivels releasing ADP and shortening sarcomere (power stroke) ATP binds to Myosin → release of myosin from actin ATP broken down to ADP & Pi → activates myosin head to bind and start again Repeats as long as Ca2+ concentration is high Copyright 2010, John Wiley & Sons, Inc. Contraction Cycle Copyright 2010, John Wiley & Sons, Inc. Relaxation Breakdown of ACh to stop muscle action potentials Ca2+ ions transported back into SR lowering concentration → This takes ATP Tropomyosin covers actin binding sites Copyright 2010, John Wiley & Sons, Inc. Muscle Tone Even at rest some motor neuron activity occurs = Muscle Tone If nerves are cut fiber becomes flaccid (very limp) Copyright 2010, John Wiley & Sons, Inc. Metabolism Rapid changes from very low ATP consumption to high levels of consumption Creatine phosphate (high energy store) Fast and good for ~ 15 sec Copyright 2010, John Wiley & Sons, Inc. Nerve impulse 1 Nerve impulse arrives at axon terminal of motor neuron and triggers release of acetylcholine (ACh). 2 ACh diffuses across synaptic cleft, binds to its receptors in the motor end plate, and triggers a muscle action potential (AP). ACh receptor 3 Acetylcholinesterase in Synaptic vesicle synaptic cleft destroys filled with ACh ACh so another muscle action potential does not arise unless more ACh is released from motor neuron. Muscle action potential Transverse tubule 4 Muscle AP travelling along transverse tubule opens Ca2+ release channels in the sarcoplasmic reticulum (SR) membrane, which allows calcium ions to flood into the sarcoplasm. SR Ca2+ 9 Muscle relaxes. 8 Troponin–tropomyosin complex slides back into position where it blocks the myosin binding sites on actin. 5 Ca2+ binds to troponin on the thin filament, exposing the binding sites for myosin. Elevated Ca2+ Ca2+ active transport pumps 7 Ca2+ release channels in SR close and Ca2+ active transport pumps use ATP to restore low level of Ca2+ in sarcoplasm. 6 Contraction: power strokes use ATP; myosin heads bind to actin, swivel, and release; thin filaments are pulled toward center of sarcomere. Copyright 2010, John Wiley & Sons, Inc. Production of ATP for Muscle Contraction Copyright 2010, John Wiley & Sons, Inc. Glycolysis Break down glucose to 2 pyruvates getting 2 ATPs If insufficient mitochondria or oxygen, pyruvate → lactic acid Get about 30–40 seconds more activity maximally Copyright 2010, John Wiley & Sons, Inc. Production of ATP for Muscle Contraction Copyright 2010, John Wiley & Sons, Inc. Aerobic Cellular Respiration Production of ATP in mitochondria Requires oxygen and carbon substrate Produces CO2 and H2O and heat. Copyright 2010, John Wiley & Sons, Inc. Fatigue Inability to contract forcefully after prolonged activity Limiting factors can include: Ca2+ Creatine Phosphate Oxygen Build up of acid Neuronal failure Copyright 2010, John Wiley & Sons, Inc. Oxygen Use After Exercise Convert lactic acid back to glucose in liver Resynthesize creatine phosphate and ATP Replace oxygen removed from myoglobin Copyright 2010, John Wiley & Sons, Inc. Control of Muscle Contraction Single action potential(AP) → twitch Total tension of fiber depends on frequency of APs (number/second) Smaller than maximum muscle force Require wave summation Maximum = tetanus Total tension of muscle depends on number of fibers contracting in unison Increasing numbers = Motor unit recruitment Copyright 2010, John Wiley & Sons, Inc. Myogram Copyright 2010, John Wiley & Sons, Inc. Myogram Copyright 2010, John Wiley & Sons, Inc. Fiber Types Slow oxidative (SO)- small diameter and red Fast oxidative- glycolytic (FOG) Large amounts of myoglobin and mitochondria ATP production primarily oxidative Fatigue resistant Large diameter = many myofibrils Many mitochondria and high glycolytic capacity Fast glycolytic fibers (FG) White, fast & powerful and fast fatiguing For strong, short term use Copyright 2010, John Wiley & Sons, Inc. Recruitment Muscle contractions only use the fibers required for the work Recruited in order: SO → FOG → FG Copyright 2010, John Wiley & Sons, Inc. Effects of Exercise SO/FG fiber ratio genetically determined Endurance exercise gives FG → FOG High FG → sprinters High SO → marathoners Increased diameter and numbers of mitochondria Strength exercise increases size and strength of FG fibers Copyright 2010, John Wiley & Sons, Inc. Cardiac Muscle Involuntary muscle found only in heart wall Striated, branched short fibers with single, central nucleus in each fiber Fibers connected by: Intercalated discs (thickened cell membranes) Gap junctions that allow spread of action potentials ATP generated by abundant mitochondria and by lactic acid when cells lack oxygen Copyright 2010, John Wiley & Sons, Inc. Cardiac Muscle Does not require nerve stimulation nerve Has its own intrinsic pacemaker (and conduction system within cardiac muscle) that initiates cardiac contraction Known as autorhythmicity Ca2+released from S.R. and extracellular spaces Intercalated discs with gap junctions transmit action potentials from ne muscle cell to the next Copyright 2010, John Wiley & Sons, Inc. Cardiac Muscle Copyright 2010, John Wiley & Sons, Inc. Smooth Muscle Involuntary Found in internal organs such as stomach, bladder, walls of arteries Structure Tapered cells each with single nucleus Filaments not regular so tissue does not appear striated Copyright 2010, John Wiley & Sons, Inc. Smooth Muscle Types Visceral (single unit) type or Multi-unit type Form sheets and are autorhythmic Contract as a unit Each has own nerve and can contract independently Graded contractions and slow responses Often sustain long term tone Often triggered by autonomic nerves Modulated chemically, by nerves, by mechanical events (stretching) Copyright 2010, John Wiley & Sons, Inc. Smooth Muscle Copyright 2010, John Wiley & Sons, Inc. Aging As with bone there is a slow progressive loss of skeletal muscle mass Relative number of SO fibers tends to increase Copyright 2010, John Wiley & Sons, Inc. Movement Muscles move one bone relative to another around one or more joint(s) Origin → most stationary end Insertion → most mobile end Location where the tendon attaches Location where tendon inserts Action → the motion or function of the muscle Copyright 2010, John Wiley & Sons, Inc. Skeletal Muscle and Bones Copyright 2010, John Wiley & Sons, Inc. Movement Generally arranged in opposing pairs Flexors - extensors; abductors - adductors The major actor: prime mover or agonist Muscle with opposite effect: antagonist Synergists - help prime mover Fixators - stabilize origin of prime mover Role of muscle varies with motion Copyright 2010, John Wiley & Sons, Inc. Basis of Muscle Names: Table 8.2 Direction of fibers relative to body axes Size of muscle Examples: lateralis, medialis (medius), intermedius, rectus Examples: alba, brevis, longus, magnus, vastus Shape of muscle Examples: deltoid, orbicularis, serratus, trapezius Copyright 2010, John Wiley & Sons, Inc. Basis of Muscle Names: Table 8.2 Action of muscle Number of tendons (heads) of origin Examples: abductor, adductor, flexor, extensor Examples: biceps, triceps, quadriceps Location of muscle Examples: abdominus, brachialis, cleido, oculo-, uro-, Copyright 2010, John Wiley & Sons, Inc. Superficial Skeletal Muscles Copyright 2010, John Wiley & Sons, Inc. Superficial Skeletal Muscles Copyright 2010, John Wiley & Sons, Inc. Muscles of the Head Copyright 2010, John Wiley & Sons, Inc. Muscles of the Eyeball Copyright 2010, John Wiley & Sons, Inc. Muscles of the Abdomen Copyright 2010, John Wiley & Sons, Inc. Muscles of the Abdomen Copyright 2010, John Wiley & Sons, Inc. Muscles of the Thorax Copyright 2010, John Wiley & Sons, Inc. Muscles of the Thorax Copyright 2010, John Wiley & Sons, Inc. Muscles of the Thorax Copyright 2010, John Wiley & Sons, Inc. Muscles of the Thorax Copyright 2010, John Wiley & Sons, Inc. Muscles of the Thorax Copyright 2010, John Wiley & Sons, Inc. Muscles of the Arm Copyright 2010, John Wiley & Sons, Inc. Muscles of the Arm Copyright 2010, John Wiley & Sons, Inc. Muscles of the Forearm Copyright 2010, John Wiley & Sons, Inc. Muscles of the Forearm Copyright 2010, John Wiley & Sons, Inc. Muscles of the Neck and Back Copyright 2010, John Wiley & Sons, Inc. Muscles of the Gluteal Region Copyright 2010, John Wiley & Sons, Inc. Muscles of the Gluteal Region Copyright 2010, John Wiley & Sons, Inc. Muscles of the Leg: Foot and Toes Copyright 2010, John Wiley & Sons, Inc. Muscles of the Leg: Foot and Toes Copyright 2010, John Wiley & Sons, Inc. End of Chapter 8 Copyright 2010 John Wiley & Sons, Inc. All rights reserved. Reproduction or translation of this work beyond that permitted in section 117 of the 1976 United States Copyright Act without express permission of the copyright owner is unlawful. Request for further information should be addressed to the Permission Department, John Wiley & Sons, Inc. The purchaser may make back-up copies for his/her own use only and not for distribution or resale. The Publishers assumes no responsibility for errors, omissions, or damages caused by the use of theses programs or from the use of the information herein. Copyright 2010, John Wiley & Sons, Inc.